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- W3048201120 abstract "Abstract Lipid composition of liposomal bilayer should alter the cell response for permeability, transport, and uptake in small intestine. This work was done to investigate the transport and uptake of liposomes composed of docosahexaenoic acid‐enriched phosphatidylcholine (PtdCho), phosphatidylserine (PtdSer), and sulfoquinovosyl diacylglycerol (SQDG) derived from marine products on multilamellar vesicles (MLV) in small intestinal epithelial cell models. The results showed that addition of PtdSer and SQDG as liposomal bilayer could improve the efficiency entrapment of liposomes. The liposomes containing PtdSer showed higher transport and uptake through both Caco‐2 cell and M cell monolayers as compared to PtdCho‐MLV. SQDG‐containing liposomes exhibited only higher transport through M cell monolayer, while its uptake effect was higher both in Caco‐2 cell and M cell monolayers. The results of experiments done with endocytosis inhibitors indicated that PtdCho‐MLV must be transported via macropinocytosis and uptaken by phagocytosis in M cell monolayer model. PtdCho/PtdSer‐MLV and PtdCho/SQDG‐MLV might be transported and uptaken through M cell monolayer by phagocytosis. The result also indicated that PtdCho/SQDG‐MLV could open the tight junction of small intestinal epithelial cell monolayers. Furthermore, our findings demonstrated that the surface status of cholesterol‐containing liposomes were smooth, but they did not affect their transport and uptake through Caco‐2 cell and M cell monolayers." @default.
- W3048201120 created "2020-08-13" @default.
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- W3048201120 date "2020-08-08" @default.
- W3048201120 modified "2023-10-11" @default.
- W3048201120 title "Lipid Composition of Liposomal Membrane Largely Affects Its Transport and Uptake through Small Intestinal Epithelial Cell Models" @default.
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- W3048201120 doi "https://doi.org/10.1002/lipd.12269" @default.
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