FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048247429> ?p ?o ?g. }

• W3048247429 abstract "Human microbiome studies remain focused on bacteria, as they comprise the dominant component of the microbiota. Recent advances in sequencing technology and optimization of amplicon sequencing protocols have allowed the description of other members of the microbiome, including eukaryotes (fungi) and, most recently, archaea. There are no known human-associated archaeal pathogens. Their diversity and contribution to health and chronic respiratory diseases, such as chronic rhinosinusitis (CRS), are unknown. Patients with CRS suffer from long-term sinus infections, and while the microbiota is hypothesized to play a role in its pathogenesis, the exact mechanism is poorly understood. In this cross-sectional study, we applied a recently optimized protocol to describe the prevalence, diversity and abundance of archaea in swab samples from the middle meatus of 60 individuals with and without CRS. A nested PCR approach was used to amplify the archaeal 16S rRNA gene for sequencing, and bacterial and archaeal load (also based on 16S rRNA genes) were estimated using Droplet Digital™ PCR (ddPCR). A total of 16 archaeal amplicon sequence variants (ASVs) from the phyla Euryarchaeota and Thaumarchaeota were identified. Archaeal ASVs were detected in 7/60 individuals, independent of disease state, whereas bacterial ASVs were detected in 60/60. Bacteria were also significantly more abundant than archaea. The ddPCR method was more sensitive than amplicon sequencing at detecting archaeal DNA in samples. Phylogenetic trees were constructed to visualize the evolutionary relationships between archaeal ASVs, isolates and clones. ASVs were placed into phylogenetic clades containing an apparent paucity of human-associated reference sequences, revealing how little studied the human archaeome is. This is the largest study to date to examine the human respiratory-associated archaeome, and provides the first insights into the prevalence, diversity and abundance of archaea in the human sinuses." @default.
• W3048247429 created "2020-08-13" @default.
• W3048247429 creator A5005419095 @default.
• W3048247429 creator A5005845333 @default.
• W3048247429 creator A5014704218 @default.
• W3048247429 creator A5015802646 @default.
• W3048247429 creator A5028144473 @default.
• W3048247429 creator A5059395368 @default.
• W3048247429 creator A5087530975 @default.
• W3048247429 date "2020-08-06" @default.
• W3048247429 modified "2023-09-27" @default.
• W3048247429 title "A Novel Description of the Human Sinus Archaeome During Health and Chronic Rhinosinusitis" @default.
• W3048247429 cites W1689013859 @default.
• W3048247429 cites W1834855158 @default.
• W3048247429 cites W1972260976 @default.
• W3048247429 cites W1972308191 @default.
• W3048247429 cites W1975533606 @default.
• W3048247429 cites W1981599889 @default.
• W3048247429 cites W1982652385 @default.
• W3048247429 cites W1996453654 @default.
• W3048247429 cites W2004690636 @default.
• W3048247429 cites W2025222272 @default.
• W3048247429 cites W2056279562 @default.
• W3048247429 cites W2064468442 @default.
• W3048247429 cites W2086268256 @default.
• W3048247429 cites W2094244872 @default.
• W3048247429 cites W2098774480 @default.
• W3048247429 cites W2110345840 @default.
• W3048247429 cites W2110773746 @default.
• W3048247429 cites W2111647009 @default.
• W3048247429 cites W2114392707 @default.
• W3048247429 cites W2126218947 @default.
• W3048247429 cites W2126989850 @default.
• W3048247429 cites W2140843031 @default.
• W3048247429 cites W2155806125 @default.
• W3048247429 cites W2166078786 @default.
• W3048247429 cites W2254642444 @default.
• W3048247429 cites W2296527675 @default.
• W3048247429 cites W2394897061 @default.
• W3048247429 cites W2547397706 @default.
• W3048247429 cites W2554506152 @default.
• W3048247429 cites W2557580049 @default.
• W3048247429 cites W2558524460 @default.
• W3048247429 cites W2603593159 @default.
• W3048247429 cites W2611324209 @default.
• W3048247429 cites W2612824952 @default.
• W3048247429 cites W2613603843 @default.
• W3048247429 cites W2625157366 @default.
• W3048247429 cites W2731624152 @default.
• W3048247429 cites W2747680554 @default.
• W3048247429 cites W2756958651 @default.
• W3048247429 cites W2768095751 @default.
• W3048247429 cites W2789156667 @default.
• W3048247429 cites W2800588250 @default.
• W3048247429 cites W2903906519 @default.
• W3048247429 cites W2912558194 @default.
• W3048247429 cites W2915662917 @default.
• W3048247429 cites W2922177283 @default.
• W3048247429 cites W2927961455 @default.
• W3048247429 cites W2946123871 @default.
• W3048247429 cites W2962743648 @default.
• W3048247429 cites W2972077277 @default.
• W3048247429 cites W2999925602 @default.
• W3048247429 cites W3103320796 @default.
• W3048247429 cites W4254687493 @default.
• W3048247429 doi "https://doi.org/10.3389/fcimb.2020.00398" @default.
• W3048247429 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7423975" @default.
• W3048247429 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32850496" @default.
• W3048247429 hasPublicationYear "2020" @default.
• W3048247429 type Work @default.
• W3048247429 sameAs 3048247429 @default.
• W3048247429 citedByCount "6" @default.
• W3048247429 countsByYear W30482474292022 @default.
• W3048247429 countsByYear W30482474292023 @default.
• W3048247429 crossrefType "journal-article" @default.
• W3048247429 hasAuthorship W3048247429A5005419095 @default.
• W3048247429 hasAuthorship W3048247429A5005845333 @default.
• W3048247429 hasAuthorship W3048247429A5014704218 @default.
• W3048247429 hasAuthorship W3048247429A5015802646 @default.
• W3048247429 hasAuthorship W3048247429A5028144473 @default.
• W3048247429 hasAuthorship W3048247429A5059395368 @default.
• W3048247429 hasAuthorship W3048247429A5087530975 @default.
• W3048247429 hasBestOaLocation W30482474291 @default.
• W3048247429 hasConcept C104317684 @default.
• W3048247429 hasConcept C143121216 @default.
• W3048247429 hasConcept C15151743 @default.
• W3048247429 hasConcept C174160445 @default.
• W3048247429 hasConcept C193252679 @default.
• W3048247429 hasConcept C2778660952 @default.
• W3048247429 hasConcept C2779258044 @default.
• W3048247429 hasConcept C42062724 @default.
• W3048247429 hasConcept C44465124 @default.
• W3048247429 hasConcept C49105822 @default.
• W3048247429 hasConcept C54355233 @default.
• W3048247429 hasConcept C550995028 @default.
• W3048247429 hasConcept C67905577 @default.
• W3048247429 hasConcept C78458016 @default.
• W3048247429 hasConcept C8185291 @default.
• W3048247429 hasConcept C86803240 @default.
• W3048247429 hasConcept C89423630 @default.

• next