FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048276143> ?p ?o ?g. }

• W3048276143 endingPage "7536" @default.
• W3048276143 startingPage "7525" @default.
• W3048276143 abstract "Far-field fluorescence localization nanoscopy of individual fluorophores at a temperature of 1.8 K was demonstrated using DNA origami as a one-nanometer-accurate scaffold. Red and near-infrared fluorophores were modified to the scaffold, and the fluorophores were 11 or 77 nm apart. We performed the localization nanoscopy of these two fluorophores at 1.8 K with a far-field fluorescence microscope. Under the cryogenic conditions, the fluorophores were perfectly immobilized and their photobleaching was drastically suppressed; consequently, the lateral spatial precision (a measure of reproducibility) was increased to 1 nm. However, the lateral spatial accuracy (a measure of trueness) remained tens of nanometers. We observed that the fluorophore centroids were laterally shifted as a function of the axial position. Because the orientation of the transition dipole of the fluorophores was fixed under cryogenic conditions, the anisotropic emission from the single fixed dipole had led to the lateral shift. This systematic error due to the dipole-orientation effect could be corrected by the three-dimensional localization of the individual fluorophores with spatial precisions of (lateral) 1 nm and (axial) 17 nm. In addition, the xy-error arising from the three-dimensional (3D) orientation of the scaffold with the two fluorophores 11 nm apart was estimated to be 0.3 nm. As a result, the individual fluorophores on the DNA origami were localized at the designed position, and the lateral spatial accuracy was quantified to be 4 nm in the standard error." @default.
• W3048276143 created "2020-08-13" @default.
• W3048276143 creator A5012515260 @default.
• W3048276143 creator A5018662166 @default.
• W3048276143 creator A5032043683 @default.
• W3048276143 creator A5040210943 @default.
• W3048276143 creator A5040463054 @default.
• W3048276143 creator A5059823579 @default.
• W3048276143 creator A5075597239 @default.
• W3048276143 date "2020-08-07" @default.
• W3048276143 modified "2023-10-06" @default.
• W3048276143 title "Cryogenic Far-Field Fluorescence Nanoscopy: Evaluation with DNA Origami" @default.
• W3048276143 cites W1606142774 @default.
• W3048276143 cites W1964599873 @default.
• W3048276143 cites W1964818179 @default.
• W3048276143 cites W1992858116 @default.
• W3048276143 cites W1998001177 @default.
• W3048276143 cites W2010932144 @default.
• W3048276143 cites W2013679989 @default.
• W3048276143 cites W2014572457 @default.
• W3048276143 cites W2018882783 @default.
• W3048276143 cites W2020198735 @default.
• W3048276143 cites W2027220406 @default.
• W3048276143 cites W2028120697 @default.
• W3048276143 cites W2028514924 @default.
• W3048276143 cites W2030000553 @default.
• W3048276143 cites W2037000609 @default.
• W3048276143 cites W2042848998 @default.
• W3048276143 cites W2051548239 @default.
• W3048276143 cites W2054455698 @default.
• W3048276143 cites W2062552264 @default.
• W3048276143 cites W2066223556 @default.
• W3048276143 cites W2070353646 @default.
• W3048276143 cites W2080894259 @default.
• W3048276143 cites W2089550950 @default.
• W3048276143 cites W2101336703 @default.
• W3048276143 cites W2103304946 @default.
• W3048276143 cites W2104045312 @default.
• W3048276143 cites W2126961173 @default.
• W3048276143 cites W2129585674 @default.
• W3048276143 cites W2136458662 @default.
• W3048276143 cites W2136683606 @default.
• W3048276143 cites W2138480914 @default.
• W3048276143 cites W2147081567 @default.
• W3048276143 cites W2151366879 @default.
• W3048276143 cites W2155919146 @default.
• W3048276143 cites W2164044612 @default.
• W3048276143 cites W2171820152 @default.
• W3048276143 cites W2291928603 @default.
• W3048276143 cites W2318588043 @default.
• W3048276143 cites W2398839780 @default.
• W3048276143 cites W2569011721 @default.
• W3048276143 cites W2584476676 @default.
• W3048276143 cites W2592829457 @default.
• W3048276143 cites W2624786346 @default.
• W3048276143 cites W2685588640 @default.
• W3048276143 cites W2794077419 @default.
• W3048276143 cites W2962579735 @default.
• W3048276143 cites W2969274740 @default.
• W3048276143 cites W2974046468 @default.
• W3048276143 cites W2975777000 @default.
• W3048276143 cites W2024317878 @default.
• W3048276143 doi "https://doi.org/10.1021/acs.jpcb.0c04721" @default.
• W3048276143 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32790384" @default.
• W3048276143 hasPublicationYear "2020" @default.
• W3048276143 type Work @default.
• W3048276143 sameAs 3048276143 @default.
• W3048276143 citedByCount "3" @default.
• W3048276143 countsByYear W30482761432022 @default.
• W3048276143 crossrefType "journal-article" @default.
• W3048276143 hasAuthorship W3048276143A5012515260 @default.
• W3048276143 hasAuthorship W3048276143A5018662166 @default.
• W3048276143 hasAuthorship W3048276143A5032043683 @default.
• W3048276143 hasAuthorship W3048276143A5040210943 @default.
• W3048276143 hasAuthorship W3048276143A5040463054 @default.
• W3048276143 hasAuthorship W3048276143A5059823579 @default.
• W3048276143 hasAuthorship W3048276143A5075597239 @default.
• W3048276143 hasConcept C118630614 @default.
• W3048276143 hasConcept C120665830 @default.
• W3048276143 hasConcept C121332964 @default.
• W3048276143 hasConcept C147080431 @default.
• W3048276143 hasConcept C16345878 @default.
• W3048276143 hasConcept C169274487 @default.
• W3048276143 hasConcept C171250308 @default.
• W3048276143 hasConcept C173523689 @default.
• W3048276143 hasConcept C178790620 @default.
• W3048276143 hasConcept C185592680 @default.
• W3048276143 hasConcept C186187911 @default.
• W3048276143 hasConcept C192562407 @default.
• W3048276143 hasConcept C25227671 @default.
• W3048276143 hasConcept C2524010 @default.
• W3048276143 hasConcept C2778617687 @default.
• W3048276143 hasConcept C31332276 @default.
• W3048276143 hasConcept C33923547 @default.
• W3048276143 hasConcept C67649825 @default.
• W3048276143 hasConcept C85725439 @default.
• W3048276143 hasConcept C91881484 @default.
• W3048276143 hasConceptScore W3048276143C118630614 @default.

• next