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• W3048296090 endingPage "101676" @default.
• W3048296090 startingPage "101676" @default.
• W3048296090 abstract "UCH-L1 is a deubiquitinating enzyme (DUB), highly abundant in neurons, with a sub-cellular localization dependent on its farnesylation state. Despite UCH-L1′s association with familial Parkinson's Disease (PD), the effects on mitochondrial bioenergetics and quality control remain unexplored. Here we investigated the role of UCHL-1 in mitochondrial dynamics and bioenergetics. We demonstrate that knock-down (KD) of UCH-L1 in different cell lines reduces the levels of the mitochondrial fusion protein Mitofusin-2, but not Mitofusin-1, resulting in mitochondrial enlargement and disruption of the tubular network. This was associated with lower tethering between mitochondria and the endoplasmic reticulum, consequently altering mitochondrial calcium uptake. Respiratory function was also altered, as UCH-L1 KD cells displayed higher proton leak and maximum respiratory capacity. Conversely, overexpression of UCH-L1 increased Mfn2 levels, an effect dramatically enhanced by the mutation of the farnesylation site (C220S), which drives UCH-L1 binding to membranes. These data indicate that the soluble cytosolic form of UCH-L1 regulates Mitofusin-2 levels and mitochondrial function. These effects are biologically conserved, since knock-down of the corresponding UCH-L1 ortholog in D. melanogaster reduces levels of the mitofusin ortholog Marf and also increases mitochondrial respiratory capacity. We thus show that Mfn-2 levels are directly affected by UCH-L1, demonstrating that the mitochondrial roles of DUBs go beyond controlling mitophagy rates." @default.
• W3048296090 created "2020-08-13" @default.
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• W3048296090 date "2020-10-01" @default.
• W3048296090 modified "2023-10-01" @default.
• W3048296090 title "A new target for an old DUB: UCH-L1 regulates mitofusin-2 levels, altering mitochondrial morphology, function and calcium uptake" @default.
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• W3048296090 cites W1618846043 @default.
• W3048296090 cites W1678211544 @default.
• W3048296090 cites W1840395037 @default.
• W3048296090 cites W1856174113 @default.
• W3048296090 cites W1864669235 @default.
• W3048296090 cites W1966686511 @default.
• W3048296090 cites W1967573239 @default.
• W3048296090 cites W1973926398 @default.
• W3048296090 cites W1975488633 @default.
• W3048296090 cites W1977860801 @default.
• W3048296090 cites W1979645469 @default.
• W3048296090 cites W1981762416 @default.
• W3048296090 cites W1987951773 @default.
• W3048296090 cites W1991959810 @default.
• W3048296090 cites W1993576291 @default.
• W3048296090 cites W2004442859 @default.
• W3048296090 cites W2015536770 @default.
• W3048296090 cites W2019455536 @default.
• W3048296090 cites W2031318456 @default.
• W3048296090 cites W2033455479 @default.
• W3048296090 cites W2034087770 @default.
• W3048296090 cites W2036468298 @default.
• W3048296090 cites W2041964828 @default.
• W3048296090 cites W2049612563 @default.
• W3048296090 cites W2049958342 @default.
• W3048296090 cites W2051476652 @default.
• W3048296090 cites W2052199849 @default.
• W3048296090 cites W2058065156 @default.
• W3048296090 cites W2059531568 @default.
• W3048296090 cites W2059654540 @default.
• W3048296090 cites W2065324486 @default.
• W3048296090 cites W2068621468 @default.
• W3048296090 cites W2073095545 @default.
• W3048296090 cites W2075119852 @default.
• W3048296090 cites W2095619100 @default.
• W3048296090 cites W2095964727 @default.
• W3048296090 cites W2097704104 @default.
• W3048296090 cites W2101362932 @default.
• W3048296090 cites W2102075074 @default.
• W3048296090 cites W2104412320 @default.
• W3048296090 cites W2106444069 @default.
• W3048296090 cites W2109692759 @default.
• W3048296090 cites W2117041194 @default.
• W3048296090 cites W2120511656 @default.
• W3048296090 cites W2121330845 @default.
• W3048296090 cites W2127806759 @default.
• W3048296090 cites W2137580945 @default.
• W3048296090 cites W2138311396 @default.
• W3048296090 cites W2140488719 @default.
• W3048296090 cites W2140679396 @default.
• W3048296090 cites W2143906078 @default.
• W3048296090 cites W2144171388 @default.
• W3048296090 cites W2144846851 @default.
• W3048296090 cites W2149363349 @default.
• W3048296090 cites W2150419930 @default.
• W3048296090 cites W2156791414 @default.
• W3048296090 cites W2166432730 @default.
• W3048296090 cites W2167031568 @default.
• W3048296090 cites W2289016169 @default.
• W3048296090 cites W2469871926 @default.
• W3048296090 cites W2515462226 @default.
• W3048296090 cites W2521680809 @default.
• W3048296090 cites W2558143992 @default.
• W3048296090 cites W2608366497 @default.
• W3048296090 cites W2617783382 @default.
• W3048296090 cites W2618356002 @default.
• W3048296090 cites W2785105763 @default.
• W3048296090 cites W2790393414 @default.
• W3048296090 cites W2805364541 @default.
• W3048296090 cites W2889614577 @default.
• W3048296090 cites W2908957874 @default.
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• W3048296090 cites W2921947689 @default.
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• W3048296090 doi "https://doi.org/10.1016/j.redox.2020.101676" @default.
• W3048296090 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7509235" @default.
• W3048296090 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32956978" @default.
• W3048296090 hasPublicationYear "2020" @default.
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