FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048298631> ?p ?o ?g. }

• W3048298631 endingPage "9284" @default.
• W3048298631 startingPage "9273" @default.
• W3048298631 abstract "Abstract Nucleic acid-binding proteins of the Sac10b family, also known as Alba, are widely distributed in Archaea. However, the physiological roles of these proteins have yet to be clarified. Here, we show that Sis10b, a member of the Sac10b family from the hyperthermophilic archaeon Sulfolobus islandicus, was active in RNA strand exchange, duplex RNA unwinding in vitro and RNA unfolding in a heterologous host cell. This protein exhibited temperature-dependent binding preference for ssRNA over dsRNA and was more efficient in RNA unwinding and RNA unfolding at elevated temperatures. Notably, alanine substitution of a highly conserved basic residue (K) at position 17 in Sis10b drastically reduced the ability of this protein to catalyse RNA strand exchange and RNA unwinding. Additionally, the preferential binding of Sis10b to ssRNA also depended on the presence of K17 or R17. Furthermore, normal growth was restored to a slow-growing Sis10b knockdown mutant by overproducing wild-type Sis10b but not by overproducing K17A in this mutant strain. Our results indicate that Sis10b is an RNA chaperone that likely functions most efficiently at temperatures optimal for the growth of S. islandicus, and K17 is essential for the chaperone activity of the protein." @default.
• W3048298631 created "2020-08-13" @default.
• W3048298631 creator A5017712497 @default.
• W3048298631 creator A5055301255 @default.
• W3048298631 creator A5086019813 @default.
• W3048298631 date "2020-08-06" @default.
• W3048298631 modified "2023-10-16" @default.
• W3048298631 title "The Sac10b homolog from Sulfolobus islandicus is an RNA chaperone" @default.
• W3048298631 cites W146609594 @default.
• W3048298631 cites W1775749144 @default.
• W3048298631 cites W1936728507 @default.
• W3048298631 cites W1966361243 @default.
• W3048298631 cites W1966913029 @default.
• W3048298631 cites W1969724537 @default.
• W3048298631 cites W1974935438 @default.
• W3048298631 cites W1987674601 @default.
• W3048298631 cites W1996446827 @default.
• W3048298631 cites W2002412136 @default.
• W3048298631 cites W2004351713 @default.
• W3048298631 cites W2005067443 @default.
• W3048298631 cites W2005085563 @default.
• W3048298631 cites W2008875170 @default.
• W3048298631 cites W2009271441 @default.
• W3048298631 cites W2013381027 @default.
• W3048298631 cites W2015591844 @default.
• W3048298631 cites W2024831748 @default.
• W3048298631 cites W2027143159 @default.
• W3048298631 cites W2030855533 @default.
• W3048298631 cites W2036116609 @default.
• W3048298631 cites W2038144055 @default.
• W3048298631 cites W2046728319 @default.
• W3048298631 cites W2046857771 @default.
• W3048298631 cites W2048571274 @default.
• W3048298631 cites W2048842540 @default.
• W3048298631 cites W2055580155 @default.
• W3048298631 cites W2058868440 @default.
• W3048298631 cites W2064596229 @default.
• W3048298631 cites W2065002182 @default.
• W3048298631 cites W2067257071 @default.
• W3048298631 cites W2068022845 @default.
• W3048298631 cites W2076998823 @default.
• W3048298631 cites W2083902847 @default.
• W3048298631 cites W2089680188 @default.
• W3048298631 cites W2092802254 @default.
• W3048298631 cites W2095745609 @default.
• W3048298631 cites W2098779579 @default.
• W3048298631 cites W2102945860 @default.
• W3048298631 cites W2112780703 @default.
• W3048298631 cites W2120101644 @default.
• W3048298631 cites W2121627025 @default.
• W3048298631 cites W2125057177 @default.
• W3048298631 cites W2139904717 @default.
• W3048298631 cites W2150535479 @default.
• W3048298631 cites W2151653586 @default.
• W3048298631 cites W2156755370 @default.
• W3048298631 cites W2159843086 @default.
• W3048298631 cites W2162356231 @default.
• W3048298631 cites W2163909810 @default.
• W3048298631 cites W2167790255 @default.
• W3048298631 cites W2171022076 @default.
• W3048298631 cites W2277292710 @default.
• W3048298631 cites W2336084778 @default.
• W3048298631 cites W2582446439 @default.
• W3048298631 cites W2746394295 @default.
• W3048298631 cites W2802389438 @default.
• W3048298631 doi "https://doi.org/10.1093/nar/gkaa656" @default.
• W3048298631 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7498313" @default.
• W3048298631 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32761152" @default.
• W3048298631 hasPublicationYear "2020" @default.
• W3048298631 type Work @default.
• W3048298631 sameAs 3048298631 @default.
• W3048298631 citedByCount "4" @default.
• W3048298631 countsByYear W30482986312021 @default.
• W3048298631 countsByYear W30482986312022 @default.
• W3048298631 countsByYear W30482986312023 @default.
• W3048298631 crossrefType "journal-article" @default.
• W3048298631 hasAuthorship W3048298631A5017712497 @default.
• W3048298631 hasAuthorship W3048298631A5055301255 @default.
• W3048298631 hasAuthorship W3048298631A5086019813 @default.
• W3048298631 hasBestOaLocation W30482986311 @default.
• W3048298631 hasConcept C104317684 @default.
• W3048298631 hasConcept C142724271 @default.
• W3048298631 hasConcept C143065580 @default.
• W3048298631 hasConcept C153911025 @default.
• W3048298631 hasConcept C161223559 @default.
• W3048298631 hasConcept C2775962898 @default.
• W3048298631 hasConcept C2778644216 @default.
• W3048298631 hasConcept C2780631447 @default.
• W3048298631 hasConcept C550995028 @default.
• W3048298631 hasConcept C55493867 @default.
• W3048298631 hasConcept C67705224 @default.
• W3048298631 hasConcept C71924100 @default.
• W3048298631 hasConcept C86803240 @default.
• W3048298631 hasConceptScore W3048298631C104317684 @default.
• W3048298631 hasConceptScore W3048298631C142724271 @default.
• W3048298631 hasConceptScore W3048298631C143065580 @default.
• W3048298631 hasConceptScore W3048298631C153911025 @default.
• W3048298631 hasConceptScore W3048298631C161223559 @default.

• next