Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048306512> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W3048306512 abstract "Expression of Ysc-Yop type III secretion system requires a tight regulation, which is conferred by two RNA-binding proteins YopD and CsrA. The underlying study demonstrated that expression of the transcriptional master regulator lcrF of the Ysc-Yop T3SS is controlled in intertwined feedback loops by these RNA-binding proteins.CsrA is a global acting post-transcriptional regulator that coordinates metabolic and virulence gene expression. This study demonstrated that expression of csrA is precisely controlled in an autoregulatory feedback loop, indirectly on the transcriptional and directly on the post-transcriptional level. CsrA was found to directly bind to two sites in the 5'-UTR of its transcript, from which one site is located within the ribosomal binding site. By this mechanism, CsrA was shown to block translational initiation and restrict CsrA synthesis when a certain CsrA amount is reached. Under non-secretion conditions, the RNA-binding protein YopD was shown to bind to the csrA transcript thereby masking a CsrA binding site and preventing the direct autoinhibition of csrA.Analysis of the role of CsrA and YopD in the lcrF regulation demonstrated a direct interaction of both RNA-binding proteins with the yscW-lcrF transcript. The 5'-UTR of lcrF contains an RNA-thermometer structure, which prevents ribosomal access through a base-pairing mechanism. Furthermore, this study demonstrated, CsrA interacts with the ribosomal binding site of lcrF and modulates the opening state of the RNA thermometer.Both regulators modulate the stability of the lcrF transcript. CsrA stabilizes and YopD destabilizes the lcrF mRNA. This is caused by the YopD- and CsrA-dependent control of the RNA degradosome.Furthermore, new putative CsrA targets could be identified by an interactome analysis with FLAG-tagged CsrA protein. At body host temperature, CsrA specifically interacts with tRNAs and transcripts of tRNA processing proteins.In summary, this study revealed the complex interplay of YopD and CsrA in controlling the expression of the T3SS master regulator lcrF. Both regulators are in involved in the post-transcriptional control of lcrF expression under non-secretion conditions and confer an antagonistic regulation." @default.
- W3048306512 created "2020-08-13" @default.
- W3048306512 creator A5001122072 @default.
- W3048306512 date "2018-01-09" @default.
- W3048306512 modified "2023-09-26" @default.
- W3048306512 title "RNA-based control of the expression of the type III secretion system of Yersinia pseudotuberculosis" @default.
- W3048306512 cites W1581054924 @default.
- W3048306512 cites W1601213308 @default.
- W3048306512 cites W1627203296 @default.
- W3048306512 cites W1680412016 @default.
- W3048306512 cites W1739566110 @default.
- W3048306512 cites W1965249456 @default.
- W3048306512 cites W1981901926 @default.
- W3048306512 cites W1986708244 @default.
- W3048306512 cites W2053489115 @default.
- W3048306512 cites W2117838035 @default.
- W3048306512 cites W2130908230 @default.
- W3048306512 cites W2141157874 @default.
- W3048306512 cites W2163857732 @default.
- W3048306512 cites W2289107156 @default.
- W3048306512 doi "https://doi.org/10.24355/dbbs.084-201801090930" @default.
- W3048306512 hasPublicationYear "2018" @default.
- W3048306512 type Work @default.
- W3048306512 sameAs 3048306512 @default.
- W3048306512 citedByCount "0" @default.
- W3048306512 crossrefType "dissertation" @default.
- W3048306512 hasAuthorship W3048306512A5001122072 @default.
- W3048306512 hasConcept C104317684 @default.
- W3048306512 hasConcept C105580179 @default.
- W3048306512 hasConcept C149364088 @default.
- W3048306512 hasConcept C150194340 @default.
- W3048306512 hasConcept C153911025 @default.
- W3048306512 hasConcept C165864922 @default.
- W3048306512 hasConcept C178809742 @default.
- W3048306512 hasConcept C27153228 @default.
- W3048306512 hasConcept C39685955 @default.
- W3048306512 hasConcept C41282012 @default.
- W3048306512 hasConcept C54355233 @default.
- W3048306512 hasConcept C67705224 @default.
- W3048306512 hasConcept C6929976 @default.
- W3048306512 hasConcept C86803240 @default.
- W3048306512 hasConcept C89604277 @default.
- W3048306512 hasConcept C95444343 @default.
- W3048306512 hasConceptScore W3048306512C104317684 @default.
- W3048306512 hasConceptScore W3048306512C105580179 @default.
- W3048306512 hasConceptScore W3048306512C149364088 @default.
- W3048306512 hasConceptScore W3048306512C150194340 @default.
- W3048306512 hasConceptScore W3048306512C153911025 @default.
- W3048306512 hasConceptScore W3048306512C165864922 @default.
- W3048306512 hasConceptScore W3048306512C178809742 @default.
- W3048306512 hasConceptScore W3048306512C27153228 @default.
- W3048306512 hasConceptScore W3048306512C39685955 @default.
- W3048306512 hasConceptScore W3048306512C41282012 @default.
- W3048306512 hasConceptScore W3048306512C54355233 @default.
- W3048306512 hasConceptScore W3048306512C67705224 @default.
- W3048306512 hasConceptScore W3048306512C6929976 @default.
- W3048306512 hasConceptScore W3048306512C86803240 @default.
- W3048306512 hasConceptScore W3048306512C89604277 @default.
- W3048306512 hasConceptScore W3048306512C95444343 @default.
- W3048306512 hasLocation W30483065121 @default.
- W3048306512 hasOpenAccess W3048306512 @default.
- W3048306512 hasPrimaryLocation W30483065121 @default.
- W3048306512 hasRelatedWork W1601213308 @default.
- W3048306512 hasRelatedWork W1680412016 @default.
- W3048306512 hasRelatedWork W1895859646 @default.
- W3048306512 hasRelatedWork W2001175146 @default.
- W3048306512 hasRelatedWork W2064855802 @default.
- W3048306512 hasRelatedWork W2068225536 @default.
- W3048306512 hasRelatedWork W2072240395 @default.
- W3048306512 hasRelatedWork W2086076421 @default.
- W3048306512 hasRelatedWork W2095113069 @default.
- W3048306512 hasRelatedWork W2116830935 @default.
- W3048306512 hasRelatedWork W2137483855 @default.
- W3048306512 hasRelatedWork W2147294805 @default.
- W3048306512 hasRelatedWork W2405583188 @default.
- W3048306512 hasRelatedWork W2516827557 @default.
- W3048306512 hasRelatedWork W2753358590 @default.
- W3048306512 hasRelatedWork W2756088843 @default.
- W3048306512 hasRelatedWork W3085822854 @default.
- W3048306512 hasRelatedWork W3120396345 @default.
- W3048306512 hasRelatedWork W3171313916 @default.
- W3048306512 hasRelatedWork W3178916555 @default.
- W3048306512 isParatext "false" @default.
- W3048306512 isRetracted "false" @default.
- W3048306512 magId "3048306512" @default.
- W3048306512 workType "dissertation" @default.