FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048337244> ?p ?o ?g. }

• W3048337244 abstract "Abstract The use of colistin as a last resort antimicrobial is compromised by the emergence of resistant enterobacteria with acquired determinants like mcr genes, mutations that activate the PmrAB two-component system and also by some other(s) still unknown mechanism(s). This work analyzed 74 E. coli isolates from healthy swine, turkey or bovine animals, characterizing their colistin resistance determinants. The mcr-1 gene, detected in 69 isolates, was the main determinant found among which 45% were carried by highly mobile plasmids, followed by four strains lacking previously known resistance determinants or two with mcr-4 (one in addition to mcr-1 ), whose phenotypes were not transferred by conjugation. Although a fraction of isolates carrying mcr-1 or mcr-4 genes also presented missense polymorphisms in pmrA or pmrB , constitutive activation of PmrAB was not detected, in contrast to control strains carrying mutations that confer colistin resistance. The expression of mcr genes negatively controls arnBCADTEF expression, a down-regulation that was also observed in the four isolates lacking known resistance determinants, three of them sharing the same macrorestriction and plasmid profiles. Genomic sequencing of one of these strains, isolated from a bovine in 2015, revealed a IncFII plasmid of 60 Kb encoding an arnBCADTEF operon closely related to Kluyvera ascorbata homologs. This element, named pArnT1, was cured by ethidum bromide and lost in parallel to colistin resistance. This work reveals that, besides mcr genes and chromosomal mutations, mobilization of arnBCADTEF operon represents a colistin resistance mechanism whose spread and relevance for public health should be carefully surveyed. Abstract Importance Colistin is an old antibiotic that has returned to first-line fighting against (Gram negative) microorganisms after pandemic rising of antimicrobial resistance. However, low susceptibility to colistin is also becoming spread, mainly by plasmid mobilization of one of the enzymes (encoded by mcr genes) that modify covalently the external layer (the lipid A component of the lipopolysaccharide) of bacterial envelope, interfering antibiotic effectiveness. The second enzymatic system that performs envelope modification and confers colistin resistance when overexpressed is encoded by arnBCADTEF operon, a set of seven genes with location restricted (up to now) to the chromosome of Gram negative bacteria. This work describes plasmid mobilization of this operon between enterobacteria, from Kluyvera to Escherichia coli , where a Kluyvera -like arnBCADTEF operon carried by pArnT1 might represent, besides mcr genes, a potential risk for antimicrobial therapy and might require careful surveillance." @default.
• W3048337244 created "2020-08-13" @default.
• W3048337244 creator A5000714060 @default.
• W3048337244 creator A5003822591 @default.
• W3048337244 creator A5005057154 @default.
• W3048337244 creator A5007178548 @default.
• W3048337244 creator A5008774036 @default.
• W3048337244 creator A5017216034 @default.
• W3048337244 creator A5033151543 @default.
• W3048337244 creator A5075586741 @default.
• W3048337244 creator A5081930508 @default.
• W3048337244 date "2020-08-08" @default.
• W3048337244 modified "2023-10-18" @default.
• W3048337244 title "Mobilization of a Kluyvera-like arnBCADTEF operon, besides mcr genes, confers colistin resistance to Escherichia coli isolated from healthy animals" @default.
• W3048337244 cites W1967620589 @default.
• W3048337244 cites W2018202875 @default.
• W3048337244 cites W2094720560 @default.
• W3048337244 cites W2116245384 @default.
• W3048337244 cites W2128908920 @default.
• W3048337244 cites W2130224428 @default.
• W3048337244 cites W2143325926 @default.
• W3048337244 cites W2171780940 @default.
• W3048337244 cites W2179036997 @default.
• W3048337244 cites W2265240579 @default.
• W3048337244 cites W2504872050 @default.
• W3048337244 cites W2526127963 @default.
• W3048337244 cites W2732368836 @default.
• W3048337244 cites W2742292956 @default.
• W3048337244 cites W2742755567 @default.
• W3048337244 cites W2768686043 @default.
• W3048337244 cites W2795433436 @default.
• W3048337244 cites W2887214667 @default.
• W3048337244 cites W2922256653 @default.
• W3048337244 cites W2949907272 @default.
• W3048337244 cites W2953427954 @default.
• W3048337244 cites W2987081802 @default.
• W3048337244 cites W3006688339 @default.
• W3048337244 cites W3014967890 @default.
• W3048337244 doi "https://doi.org/10.1101/2020.08.06.240812" @default.
• W3048337244 hasPublicationYear "2020" @default.
• W3048337244 type Work @default.
• W3048337244 sameAs 3048337244 @default.
• W3048337244 citedByCount "0" @default.
• W3048337244 crossrefType "posted-content" @default.
• W3048337244 hasAuthorship W3048337244A5000714060 @default.
• W3048337244 hasAuthorship W3048337244A5003822591 @default.
• W3048337244 hasAuthorship W3048337244A5005057154 @default.
• W3048337244 hasAuthorship W3048337244A5007178548 @default.
• W3048337244 hasAuthorship W3048337244A5008774036 @default.
• W3048337244 hasAuthorship W3048337244A5017216034 @default.
• W3048337244 hasAuthorship W3048337244A5033151543 @default.
• W3048337244 hasAuthorship W3048337244A5075586741 @default.
• W3048337244 hasAuthorship W3048337244A5081930508 @default.
• W3048337244 hasBestOaLocation W30483372441 @default.
• W3048337244 hasConcept C104317684 @default.
• W3048337244 hasConcept C203075996 @default.
• W3048337244 hasConcept C22744801 @default.
• W3048337244 hasConcept C2777620836 @default.
• W3048337244 hasConcept C2778910516 @default.
• W3048337244 hasConcept C2779259085 @default.
• W3048337244 hasConcept C501593827 @default.
• W3048337244 hasConcept C54355233 @default.
• W3048337244 hasConcept C547475151 @default.
• W3048337244 hasConcept C86803240 @default.
• W3048337244 hasConcept C89423630 @default.
• W3048337244 hasConceptScore W3048337244C104317684 @default.
• W3048337244 hasConceptScore W3048337244C203075996 @default.
• W3048337244 hasConceptScore W3048337244C22744801 @default.
• W3048337244 hasConceptScore W3048337244C2777620836 @default.
• W3048337244 hasConceptScore W3048337244C2778910516 @default.
• W3048337244 hasConceptScore W3048337244C2779259085 @default.
• W3048337244 hasConceptScore W3048337244C501593827 @default.
• W3048337244 hasConceptScore W3048337244C54355233 @default.
• W3048337244 hasConceptScore W3048337244C547475151 @default.
• W3048337244 hasConceptScore W3048337244C86803240 @default.
• W3048337244 hasConceptScore W3048337244C89423630 @default.
• W3048337244 hasLocation W30483372441 @default.
• W3048337244 hasOpenAccess W3048337244 @default.
• W3048337244 hasPrimaryLocation W30483372441 @default.
• W3048337244 hasRelatedWork W2515138802 @default.
• W3048337244 hasRelatedWork W2516404684 @default.
• W3048337244 hasRelatedWork W2616295139 @default.
• W3048337244 hasRelatedWork W2747942801 @default.
• W3048337244 hasRelatedWork W2765419621 @default.
• W3048337244 hasRelatedWork W2765781787 @default.
• W3048337244 hasRelatedWork W2770095722 @default.
• W3048337244 hasRelatedWork W2899178062 @default.
• W3048337244 hasRelatedWork W3007921898 @default.
• W3048337244 hasRelatedWork W4310134810 @default.
• W3048337244 isParatext "false" @default.
• W3048337244 isRetracted "false" @default.
• W3048337244 magId "3048337244" @default.
• W3048337244 workType "article" @default.