FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048421488> ?p ?o ?g. }

• W3048421488 abstract "In adult cardiomyocytes (CMs), the type 2 ryanodine receptor (RYR2) is an indispensable Ca2+ release channel that ensures the integrity of excitation-contraction coupling, which is fundamental for every heartbeat. However, the role and importance of RYR2 during human embryonic cardiac development are still poorly understood. Here, we generated two human induced pluripotent stem cell (iPSC)-based RYR2 knockout (RYR2-/-) lines using the CRISPR/Cas9 gene editing technology. We found that RYR2-/--iPSCs could differentiate into CMs with the efficiency similar to control-iPSCs (Ctrl-iPSCs); however, the survival of iPSC-CMs was markedly affected by the lack of functional RYR2. While Ctrl-iPSC-CMs exhibited regular Ca2+ handling, we observed significantly reduced frequency and intense abnormalities of Ca2+ transients in RYR2-/--iPSC-CMs. Ctrl-iPSC-CMs displayed sensitivity to extracellular Ca2+ ([Ca2+ ]o) and caffeine in a concentration-dependent manner, while RYR2-/--iPSC-CMs showed inconsistent reactions to [Ca2+ ]o and were insensitive to caffeine, indicating there is no RYR2-mediated Ca2+ release from the sarcoplasmic reticulum (SR). Instead, compensatory mechanism for calcium handling in RYR2-/--iPSC-CMs is partially mediated by the inositol 1,4,5-trisphosphate receptor (IP3R). Similar to Ctrl-iPSC-CMs, SR Ca2+ refilling in RYR2-/--iPSC-CMs is mediated by SERCA. Additionally, RYR2-/--iPSC-CMs showed a decreased beating rate and a reduced peak amplitude of L-type Ca2+ current. These findings demonstrate that RYR2 is not required for CM lineage commitment but is important for CM survival and contractile function. IP3R-mediated Ca2+ release is one of the major compensatory mechanisms for Ca2+ cycling in human CMs with the RYR2 deficiency." @default.
• W3048421488 created "2020-08-18" @default.
• W3048421488 creator A5004061115 @default.
• W3048421488 creator A5014677921 @default.
• W3048421488 creator A5014858706 @default.
• W3048421488 creator A5032380309 @default.
• W3048421488 creator A5032695729 @default.
• W3048421488 creator A5061580702 @default.
• W3048421488 creator A5072967440 @default.
• W3048421488 creator A5076376167 @default.
• W3048421488 creator A5084993007 @default.
• W3048421488 date "2020-08-12" @default.
• W3048421488 modified "2023-10-17" @default.
• W3048421488 title "IP3R-Mediated Compensatory Mechanism for Calcium Handling in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes With Cardiac Ryanodine Receptor Deficiency" @default.
• W3048421488 cites W1509858114 @default.
• W3048421488 cites W1564814502 @default.
• W3048421488 cites W1575466408 @default.
• W3048421488 cites W1963576928 @default.
• W3048421488 cites W1972265168 @default.
• W3048421488 cites W1973364701 @default.
• W3048421488 cites W1974197507 @default.
• W3048421488 cites W1998299402 @default.
• W3048421488 cites W2003142024 @default.
• W3048421488 cites W2015302374 @default.
• W3048421488 cites W2021412160 @default.
• W3048421488 cites W2022585930 @default.
• W3048421488 cites W2023295888 @default.
• W3048421488 cites W2028268498 @default.
• W3048421488 cites W2034000908 @default.
• W3048421488 cites W2050824263 @default.
• W3048421488 cites W2083599207 @default.
• W3048421488 cites W2085620992 @default.
• W3048421488 cites W2105276369 @default.
• W3048421488 cites W2109139329 @default.
• W3048421488 cites W2111867044 @default.
• W3048421488 cites W2112810806 @default.
• W3048421488 cites W2114282609 @default.
• W3048421488 cites W2117226055 @default.
• W3048421488 cites W2125987139 @default.
• W3048421488 cites W2138053038 @default.
• W3048421488 cites W2138977668 @default.
• W3048421488 cites W2141283808 @default.
• W3048421488 cites W2142046859 @default.
• W3048421488 cites W2166893144 @default.
• W3048421488 cites W2519104456 @default.
• W3048421488 cites W2549436143 @default.
• W3048421488 cites W2599411053 @default.
• W3048421488 cites W2638423511 @default.
• W3048421488 cites W2728447508 @default.
• W3048421488 cites W2793378737 @default.
• W3048421488 cites W2794616878 @default.
• W3048421488 cites W2809013388 @default.
• W3048421488 cites W2882967527 @default.
• W3048421488 cites W99404869 @default.
• W3048421488 doi "https://doi.org/10.3389/fcell.2020.00772" @default.
• W3048421488 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7434870" @default.
• W3048421488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32903370" @default.
• W3048421488 hasPublicationYear "2020" @default.
• W3048421488 type Work @default.
• W3048421488 sameAs 3048421488 @default.
• W3048421488 citedByCount "15" @default.
• W3048421488 countsByYear W30484214882020 @default.
• W3048421488 countsByYear W30484214882021 @default.
• W3048421488 countsByYear W30484214882022 @default.
• W3048421488 countsByYear W30484214882023 @default.
• W3048421488 crossrefType "journal-article" @default.
• W3048421488 hasAuthorship W3048421488A5004061115 @default.
• W3048421488 hasAuthorship W3048421488A5014677921 @default.
• W3048421488 hasAuthorship W3048421488A5014858706 @default.
• W3048421488 hasAuthorship W3048421488A5032380309 @default.
• W3048421488 hasAuthorship W3048421488A5032695729 @default.
• W3048421488 hasAuthorship W3048421488A5061580702 @default.
• W3048421488 hasAuthorship W3048421488A5072967440 @default.
• W3048421488 hasAuthorship W3048421488A5076376167 @default.
• W3048421488 hasAuthorship W3048421488A5084993007 @default.
• W3048421488 hasBestOaLocation W30484214881 @default.
• W3048421488 hasConcept C104317684 @default.
• W3048421488 hasConcept C107459253 @default.
• W3048421488 hasConcept C113217602 @default.
• W3048421488 hasConcept C126322002 @default.
• W3048421488 hasConcept C134018914 @default.
• W3048421488 hasConcept C145103041 @default.
• W3048421488 hasConcept C158617107 @default.
• W3048421488 hasConcept C185592680 @default.
• W3048421488 hasConcept C190712762 @default.
• W3048421488 hasConcept C2778500429 @default.
• W3048421488 hasConcept C519063684 @default.
• W3048421488 hasConcept C55493867 @default.
• W3048421488 hasConcept C71924100 @default.
• W3048421488 hasConcept C86803240 @default.
• W3048421488 hasConcept C95444343 @default.
• W3048421488 hasConceptScore W3048421488C104317684 @default.
• W3048421488 hasConceptScore W3048421488C107459253 @default.
• W3048421488 hasConceptScore W3048421488C113217602 @default.
• W3048421488 hasConceptScore W3048421488C126322002 @default.
• W3048421488 hasConceptScore W3048421488C134018914 @default.
• W3048421488 hasConceptScore W3048421488C145103041 @default.
• W3048421488 hasConceptScore W3048421488C158617107 @default.
• W3048421488 hasConceptScore W3048421488C185592680 @default.
• W3048421488 hasConceptScore W3048421488C190712762 @default.

• next