Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048468863> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W3048468863 endingPage "18936" @default.
- W3048468863 startingPage "18934" @default.
- W3048468863 abstract "With great interest, we read the paper by Zhou et al. (1) describing a methodology that enables extracellular RNA sequencing (exRNA-seq) from extremely low input (Small Input Liquid Volume Extracellular RNA Sequencing [SILVER-seq]). We were intrigued by the high number of detected genes compared to our previous studies (2, 3) and noticed low reproducibility. We hypothesized that these observations could originate from substantial DNA contamination. Therefore, we reanalyzed the SILVER-seq data (4) to determine the extent of DNA signal in the sequencing reads.First, we analyzed the fraction of reads mapping to the different genomic regions. We noticed that these fractions closely resembled the distributions in the genome (Fig. 1 A ). Specifically, fewer than 5% of the reads mapped to exonic regions, while our own exRNA-seq data (3) showed an average of 35% exonic reads. Secondly, we analyzed reads mapping to spliced sequences, expecting them to be … [↵][1]1To whom correspondence may be addressed. Email: Jasper.verwilt{at}ugent.be. [1]: #xref-corresp-1-1" @default.
- W3048468863 created "2020-08-18" @default.
- W3048468863 creator A5000257053 @default.
- W3048468863 creator A5008147896 @default.
- W3048468863 creator A5037781040 @default.
- W3048468863 creator A5057225515 @default.
- W3048468863 creator A5058438834 @default.
- W3048468863 creator A5082603120 @default.
- W3048468863 creator A5086580140 @default.
- W3048468863 date "2020-08-11" @default.
- W3048468863 modified "2023-10-16" @default.
- W3048468863 title "When DNA gets in the way: A cautionary note for DNA contamination in extracellular RNA-seq studies" @default.
- W3048468863 cites W2972037070 @default.
- W3048468863 cites W2990419298 @default.
- W3048468863 cites W3048468863 @default.
- W3048468863 doi "https://doi.org/10.1073/pnas.2001675117" @default.
- W3048468863 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7431080" @default.
- W3048468863 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32788394" @default.
- W3048468863 hasPublicationYear "2020" @default.
- W3048468863 type Work @default.
- W3048468863 sameAs 3048468863 @default.
- W3048468863 citedByCount "14" @default.
- W3048468863 countsByYear W30484688632020 @default.
- W3048468863 countsByYear W30484688632021 @default.
- W3048468863 countsByYear W30484688632022 @default.
- W3048468863 countsByYear W30484688632023 @default.
- W3048468863 crossrefType "journal-article" @default.
- W3048468863 hasAuthorship W3048468863A5000257053 @default.
- W3048468863 hasAuthorship W3048468863A5008147896 @default.
- W3048468863 hasAuthorship W3048468863A5037781040 @default.
- W3048468863 hasAuthorship W3048468863A5057225515 @default.
- W3048468863 hasAuthorship W3048468863A5058438834 @default.
- W3048468863 hasAuthorship W3048468863A5082603120 @default.
- W3048468863 hasAuthorship W3048468863A5086580140 @default.
- W3048468863 hasBestOaLocation W30484688631 @default.
- W3048468863 hasConcept C104317684 @default.
- W3048468863 hasConcept C107397762 @default.
- W3048468863 hasConcept C112570922 @default.
- W3048468863 hasConcept C150194340 @default.
- W3048468863 hasConcept C162317418 @default.
- W3048468863 hasConcept C18903297 @default.
- W3048468863 hasConcept C28406088 @default.
- W3048468863 hasConcept C54355233 @default.
- W3048468863 hasConcept C552990157 @default.
- W3048468863 hasConcept C67705224 @default.
- W3048468863 hasConcept C70721500 @default.
- W3048468863 hasConcept C86803240 @default.
- W3048468863 hasConceptScore W3048468863C104317684 @default.
- W3048468863 hasConceptScore W3048468863C107397762 @default.
- W3048468863 hasConceptScore W3048468863C112570922 @default.
- W3048468863 hasConceptScore W3048468863C150194340 @default.
- W3048468863 hasConceptScore W3048468863C162317418 @default.
- W3048468863 hasConceptScore W3048468863C18903297 @default.
- W3048468863 hasConceptScore W3048468863C28406088 @default.
- W3048468863 hasConceptScore W3048468863C54355233 @default.
- W3048468863 hasConceptScore W3048468863C552990157 @default.
- W3048468863 hasConceptScore W3048468863C67705224 @default.
- W3048468863 hasConceptScore W3048468863C70721500 @default.
- W3048468863 hasConceptScore W3048468863C86803240 @default.
- W3048468863 hasIssue "32" @default.
- W3048468863 hasLocation W30484688631 @default.
- W3048468863 hasLocation W30484688632 @default.
- W3048468863 hasLocation W30484688633 @default.
- W3048468863 hasOpenAccess W3048468863 @default.
- W3048468863 hasPrimaryLocation W30484688631 @default.
- W3048468863 hasRelatedWork W1986936937 @default.
- W3048468863 hasRelatedWork W2034409080 @default.
- W3048468863 hasRelatedWork W2050711297 @default.
- W3048468863 hasRelatedWork W2125434199 @default.
- W3048468863 hasRelatedWork W2327028314 @default.
- W3048468863 hasRelatedWork W2355749553 @default.
- W3048468863 hasRelatedWork W281735054 @default.
- W3048468863 hasRelatedWork W2952941127 @default.
- W3048468863 hasRelatedWork W4246525302 @default.
- W3048468863 hasRelatedWork W190756422 @default.
- W3048468863 hasVolume "117" @default.
- W3048468863 isParatext "false" @default.
- W3048468863 isRetracted "false" @default.
- W3048468863 magId "3048468863" @default.
- W3048468863 workType "article" @default.