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• W3048484676 abstract "Abstract Background Whey is a major pollutant generated by the dairy industry. To decrease environmental pollution caused by the industrial release of whey, new prospects for its utilization need to be urgently explored. Here, we investigated the possibility of using whey powder to produce 2,3-butanediol (BDO), an important platform chemical. Results Klebsiella oxytoca strain PDL-0 was selected because of its ability to efficiently produce BDO from lactose, the major fermentable sugar in whey. After deleting genes pox , pta , frdA , ldhD , and pflB responding for the production of by-products acetate, succinate, lactate, and formate, a recombinant strain K. oxytoca PDL-K5 was constructed. Fed-batch fermentation using K. oxytoca PDL-K5 produced 74.9 g/L BDO with a productivity of 2.27 g/L/h and a yield of 0.43 g/g from lactose. In addition, when whey powder was used as the substrate, 65.5 g/L BDO was produced within 24 h with a productivity of 2.73 g/L/h and a yield of 0.44 g/g. Conclusion This study demonstrated the efficiency of K. oxytoca PDL-0 for BDO production from whey. Due to its non-pathogenicity and efficient lactose utilization, K. oxytoca PDL-0 might also be used in the production of other important chemicals using whey as the substrate." @default.
• W3048484676 created "2020-08-18" @default.
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• W3048484676 date "2020-08-10" @default.
• W3048484676 modified "2023-10-13" @default.
• W3048484676 title "Efficient 2,3-butanediol production from whey powder using metabolically engineered Klebsiella oxytoca" @default.
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• W3048484676 doi "https://doi.org/10.1186/s12934-020-01420-2" @default.
• W3048484676 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7419187" @default.
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