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- W3048503492 abstract "This chapter discusses the window in passing traffic, monitoring hematopoietic cell maturation and differentiation at different levels along the stem and progenitor cell hierarchy. Limiting-dilution analysis showed that long-term culture-ICML are present in normal murine bone marrow at a frequency which is in the order of previous estimates of primitive hematopoietic stem cells by in vivo competitive repopulation assays. One month following transplantation, the femoral bone marrow of the transplanted mice was harvested, and various surrogate assays of hematopoietic stem cell activity were performed. However, analysis of transplantation models has shown that a large spectrum of hematopoietic cells with widely divergent proliferative histories and potentialities are capable of engrafting and restoring normal blood cell production in myeloablated animals. High-proliferative potential colony-forming cells are among the most primitive hematopoietic cells yet identified in vitro. Recognition of the requirement for stroma to sustain long-term–reconstituting cells in liquid culture has led to the development of long-term bone marrow culture-based assays." @default.
- W3048503492 created "2020-08-18" @default.
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- W3048503492 date "2020-08-26" @default.
- W3048503492 modified "2023-09-25" @default.
- W3048503492 title "Surrogate Assays for Hematopoietic Stem Cell Activity" @default.
- W3048503492 cites W1568449911 @default.
- W3048503492 doi "https://doi.org/10.1201/9781003067405-2" @default.
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