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- W3048550960 abstract "Intestinal ischemia reperfusion injury (iIRI) is a severe clinical condition presenting high morbidity and mortality worldwide. Some of the systemic consequences of IRI can be prevented by applying ischemic preconditioning (IPC), a series of short ischemia/reperfusion events preceding the major ischemia. Although neutrophils are key players in the pathophysiology of ischemic injuries, neither the dysregulation presented by these cells in iIRI nor the protective effect of iIPC have their regulation mechanisms fully understood. Protein phosphorylation, as well as the regulation of the respective phosphatases and kinases are responsible for regulating a large number of cellular functions in the inflammatory response. Moreover, in previous work we found hydrolases and transferases to be modulated in iIR and iIPC, suggesting the possible involvement of phosphatases and kinases in the process. Therefore, in the present study, we analyzed the phosphoproteome of neutrophils from rats submitted to mesenteric ischemia and reperfusion, either submitted or not to IPC, compared to quiescent controls and sham laparotomy. Proteomic analysis was performed by multi-step enrichment of phosphopeptides, isobaric labeling, and LC-MS/MS analysis. Bioinformatics was used to determine phosphosite and phosphopeptide abundance and clustering, as well as kinases and phosphatases sites and domains. We found that most of the phosphorylation-regulated proteins are involved in apoptosis and migration, and most of the regulatory kinases belong to CAMK and CMGC families. An interesting finding revealed groups of proteins that are modulated by iIR, but such modulation can be prevented by iIPC. Among the regulated proteins related to the iIPC protective effect, Vamp8 and Inpp5d/Ship are discussed as possible candidates for control of the iIR damage." @default.
- W3048550960 created "2020-08-18" @default.
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- W3048550960 date "2020-08-13" @default.
- W3048550960 modified "2023-10-16" @default.
- W3048550960 title "Phosphoproteomic Analysis of Rat Neutrophils Shows the Effect of Intestinal Ischemia/Reperfusion and Preconditioning on Kinases and Phosphatases" @default.
- W3048550960 cites W136427227 @default.
- W3048550960 cites W1496551055 @default.
- W3048550960 cites W1525018880 @default.
- W3048550960 cites W1538925875 @default.
- W3048550960 cites W1540273720 @default.
- W3048550960 cites W1582842326 @default.
- W3048550960 cites W1593972670 @default.
- W3048550960 cites W1634711419 @default.
- W3048550960 cites W1640591666 @default.
- W3048550960 cites W1885471961 @default.
- W3048550960 cites W1944348537 @default.
- W3048550960 cites W1964504843 @default.
- W3048550960 cites W1966496217 @default.
- W3048550960 cites W1966930760 @default.
- W3048550960 cites W1969543675 @default.
- W3048550960 cites W1970553359 @default.
- W3048550960 cites W1971900596 @default.
- W3048550960 cites W1984450835 @default.
- W3048550960 cites W1987252087 @default.
- W3048550960 cites W1989144072 @default.
- W3048550960 cites W1989707466 @default.
- W3048550960 cites W1996151753 @default.
- W3048550960 cites W1997835441 @default.
- W3048550960 cites W2003809857 @default.
- W3048550960 cites W2005339053 @default.
- W3048550960 cites W2006100483 @default.
- W3048550960 cites W2007097872 @default.
- W3048550960 cites W2007273883 @default.
- W3048550960 cites W2008356007 @default.
- W3048550960 cites W2009271645 @default.
- W3048550960 cites W2011473183 @default.
- W3048550960 cites W2012223636 @default.
- W3048550960 cites W2012698880 @default.
- W3048550960 cites W2014752437 @default.
- W3048550960 cites W2020547252 @default.
- W3048550960 cites W2023392069 @default.
- W3048550960 cites W2023538808 @default.
- W3048550960 cites W2024398910 @default.
- W3048550960 cites W2024765629 @default.
- W3048550960 cites W2026738139 @default.
- W3048550960 cites W2028125482 @default.
- W3048550960 cites W2030205108 @default.
- W3048550960 cites W2031121711 @default.
- W3048550960 cites W2035421116 @default.
- W3048550960 cites W2037199116 @default.
- W3048550960 cites W2038106901 @default.
- W3048550960 cites W2039577125 @default.
- W3048550960 cites W2039577556 @default.
- W3048550960 cites W2042020095 @default.
- W3048550960 cites W2042044881 @default.
- W3048550960 cites W2042940417 @default.
- W3048550960 cites W2044074327 @default.
- W3048550960 cites W2044732680 @default.
- W3048550960 cites W2046153742 @default.
- W3048550960 cites W2047321895 @default.
- W3048550960 cites W2049097275 @default.
- W3048550960 cites W2050090295 @default.
- W3048550960 cites W2052394407 @default.
- W3048550960 cites W2052971617 @default.
- W3048550960 cites W2053197710 @default.
- W3048550960 cites W2053625020 @default.
- W3048550960 cites W2054352267 @default.
- W3048550960 cites W2056012261 @default.
- W3048550960 cites W2057193859 @default.
- W3048550960 cites W2057861297 @default.
- W3048550960 cites W2058019844 @default.
- W3048550960 cites W2064432631 @default.
- W3048550960 cites W2066732411 @default.
- W3048550960 cites W2069312178 @default.
- W3048550960 cites W2073022274 @default.
- W3048550960 cites W2073395431 @default.
- W3048550960 cites W2073765055 @default.
- W3048550960 cites W2075352286 @default.
- W3048550960 cites W2076035297 @default.
- W3048550960 cites W2077261875 @default.
- W3048550960 cites W2079997671 @default.
- W3048550960 cites W2080944448 @default.
- W3048550960 cites W2081135891 @default.
- W3048550960 cites W2082738022 @default.
- W3048550960 cites W2083995600 @default.
- W3048550960 cites W2087548571 @default.
- W3048550960 cites W2090522535 @default.
- W3048550960 cites W2090530769 @default.
- W3048550960 cites W2091242855 @default.