FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048551323> ?p ?o ?g. }

• W3048551323 endingPage "107600" @default.
• W3048551323 startingPage "107600" @default.
• W3048551323 abstract "Compared with conventional two-dimensional transmission electron microscopy (TEM), focused ion beam scanning electron microscopy (FIB-SEM) can provide more comprehensive 3D information on cell substructures at the nanometer scale. Biological samples prepared by cryofixation using high-pressure freezing demonstrate optimal preservation of the morphology of cellular structures, as these are arrested instantly in their near-native states. However, samples from cryofixation often show a weak back-scatter electron signal and bad image contrast in FIB-SEM imaging. In addition, it is impossible to do large amounts of heavy metal staining. This is commonly achieved via established osmium impregnation (OTO) en bloc staining protocols. Here, we compared the FIB-SEM image quality of brain tissues prepared using several common freeze-substitution media, and we developed an approach that overcomes these limitations through a combination of osmium tetroxide, uranyl acetate, tannic acid, and potassium permanganate at proper concentrations, respectively. Using this optimized sample preparation protocol for high-pressure freezing and freeze-substitution, perfect smooth membrane morphology, even of the lipid bilayers of the cell membrane, was readily obtained using FIB-SEM. In addition, our protocol is broadly applicable and we demonstrated successful application to brain tissues, plant tissues, Caenorhabditis elegans, Candida albicans, and chlorella. This approach combines the potential of cryofixation for 3D large volume analysis of subcellular structures with the high-resolution capabilities of FIB-SEM." @default.
• W3048551323 created "2020-08-18" @default.
• W3048551323 creator A5004558484 @default.
• W3048551323 creator A5004693417 @default.
• W3048551323 creator A5033972281 @default.
• W3048551323 creator A5047060456 @default.
• W3048551323 creator A5051653210 @default.
• W3048551323 creator A5066084811 @default.
• W3048551323 creator A5074597310 @default.
• W3048551323 date "2020-10-01" @default.
• W3048551323 modified "2023-09-27" @default.
• W3048551323 title "An optimized approach using cryofixation for high-resolution 3D analysis by FIB-SEM" @default.
• W3048551323 cites W1513082520 @default.
• W3048551323 cites W1532431243 @default.
• W3048551323 cites W1581443166 @default.
• W3048551323 cites W1587124315 @default.
• W3048551323 cites W1926373965 @default.
• W3048551323 cites W1965637518 @default.
• W3048551323 cites W1966445035 @default.
• W3048551323 cites W1989621138 @default.
• W3048551323 cites W1990232524 @default.
• W3048551323 cites W1992898843 @default.
• W3048551323 cites W1996414800 @default.
• W3048551323 cites W1999762081 @default.
• W3048551323 cites W2003394211 @default.
• W3048551323 cites W2009939144 @default.
• W3048551323 cites W2016653518 @default.
• W3048551323 cites W2026204622 @default.
• W3048551323 cites W2029980283 @default.
• W3048551323 cites W2032612647 @default.
• W3048551323 cites W2036100004 @default.
• W3048551323 cites W2038665424 @default.
• W3048551323 cites W2053487271 @default.
• W3048551323 cites W2053982911 @default.
• W3048551323 cites W2056673093 @default.
• W3048551323 cites W2069702026 @default.
• W3048551323 cites W2072560959 @default.
• W3048551323 cites W2081259067 @default.
• W3048551323 cites W2081406232 @default.
• W3048551323 cites W2083895131 @default.
• W3048551323 cites W2093450191 @default.
• W3048551323 cites W2094827098 @default.
• W3048551323 cites W2113097520 @default.
• W3048551323 cites W2127548720 @default.
• W3048551323 cites W2155385419 @default.
• W3048551323 cites W2155833850 @default.
• W3048551323 cites W2169489226 @default.
• W3048551323 cites W2418573148 @default.
• W3048551323 cites W2480732292 @default.
• W3048551323 cites W2618988158 @default.
• W3048551323 cites W2789207897 @default.
• W3048551323 cites W2800184149 @default.
• W3048551323 cites W2898660562 @default.
• W3048551323 cites W4211114670 @default.
• W3048551323 doi "https://doi.org/10.1016/j.jsb.2020.107600" @default.
• W3048551323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32798655" @default.
• W3048551323 hasPublicationYear "2020" @default.
• W3048551323 type Work @default.
• W3048551323 sameAs 3048551323 @default.
• W3048551323 citedByCount "6" @default.
• W3048551323 countsByYear W30485513232021 @default.
• W3048551323 countsByYear W30485513232022 @default.
• W3048551323 countsByYear W30485513232023 @default.
• W3048551323 crossrefType "journal-article" @default.
• W3048551323 hasAuthorship W3048551323A5004558484 @default.
• W3048551323 hasAuthorship W3048551323A5004693417 @default.
• W3048551323 hasAuthorship W3048551323A5033972281 @default.
• W3048551323 hasAuthorship W3048551323A5047060456 @default.
• W3048551323 hasAuthorship W3048551323A5051653210 @default.
• W3048551323 hasAuthorship W3048551323A5066084811 @default.
• W3048551323 hasAuthorship W3048551323A5074597310 @default.
• W3048551323 hasConcept C120665830 @default.
• W3048551323 hasConcept C121332964 @default.
• W3048551323 hasConcept C12554922 @default.
• W3048551323 hasConcept C145148216 @default.
• W3048551323 hasConcept C146088050 @default.
• W3048551323 hasConcept C159985019 @default.
• W3048551323 hasConcept C161866238 @default.
• W3048551323 hasConcept C171250308 @default.
• W3048551323 hasConcept C178790620 @default.
• W3048551323 hasConcept C185592680 @default.
• W3048551323 hasConcept C192562407 @default.
• W3048551323 hasConcept C26771246 @default.
• W3048551323 hasConcept C2778576202 @default.
• W3048551323 hasConcept C2779424567 @default.
• W3048551323 hasConcept C2781060271 @default.
• W3048551323 hasConcept C43617362 @default.
• W3048551323 hasConcept C7869135 @default.
• W3048551323 hasConcept C86803240 @default.
• W3048551323 hasConcept C93877712 @default.
• W3048551323 hasConceptScore W3048551323C120665830 @default.
• W3048551323 hasConceptScore W3048551323C121332964 @default.
• W3048551323 hasConceptScore W3048551323C12554922 @default.
• W3048551323 hasConceptScore W3048551323C145148216 @default.
• W3048551323 hasConceptScore W3048551323C146088050 @default.
• W3048551323 hasConceptScore W3048551323C159985019 @default.
• W3048551323 hasConceptScore W3048551323C161866238 @default.
• W3048551323 hasConceptScore W3048551323C171250308 @default.

• next