FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048554174> ?p ?o ?g. }

• W3048554174 endingPage "2665" @default.
• W3048554174 startingPage "2658" @default.
• W3048554174 abstract "Prolines in signaling proteins are of particular interest because they have a range of unique properties that may be critical for function. Here we show that many proline residues in the extracellular domain (ECD) of the glycine receptor are involved in the correct functioning of this ligand-gated ion channel. We explore their role by creating mutant receptors, expressing them in cells, and using fluorescent membrane potential sensitive dye to monitor receptor activity. We then interpret the changes in receptor parameters using structural information from the open and closed states of the receptor. The data reveal that substitution with alanine of ten of the 13 Pro residues in the ECD alters the function of the receptor: one substitution ablates function, six cause a decrease in the EC50, and three cause an increase. Only three of these mutants result in EC50 values similar to WT. The nonfunctional mutant, Pro30Ala, was further probed in oocytes, and the data suggest a role in both expression and function. Examination of the locations of sensitive Pro residues in the receptor and identification of potential interactions with nearby residues reveal how these residues could contribute to the correct functioning of this typical pentameric ligand-gated ion channel." @default.
• W3048554174 created "2020-08-18" @default.
• W3048554174 creator A5019015682 @default.
• W3048554174 creator A5041256217 @default.
• W3048554174 creator A5047202581 @default.
• W3048554174 creator A5058773225 @default.
• W3048554174 date "2020-08-10" @default.
• W3048554174 modified "2023-09-23" @default.
• W3048554174 title "Many Proline Residues in the Extracellular Domain Contribute to Glycine Receptor Function" @default.
• W3048554174 cites W1550469113 @default.
• W3048554174 cites W1963766570 @default.
• W3048554174 cites W1967163463 @default.
• W3048554174 cites W1979001025 @default.
• W3048554174 cites W1983731920 @default.
• W3048554174 cites W1990397785 @default.
• W3048554174 cites W1994397958 @default.
• W3048554174 cites W1998079894 @default.
• W3048554174 cites W2003468829 @default.
• W3048554174 cites W2003538992 @default.
• W3048554174 cites W2006801299 @default.
• W3048554174 cites W2021842829 @default.
• W3048554174 cites W2027784769 @default.
• W3048554174 cites W2029076044 @default.
• W3048554174 cites W2055195337 @default.
• W3048554174 cites W2082621853 @default.
• W3048554174 cites W2083685628 @default.
• W3048554174 cites W2083959641 @default.
• W3048554174 cites W2085913208 @default.
• W3048554174 cites W2090231692 @default.
• W3048554174 cites W2098742258 @default.
• W3048554174 cites W2133675069 @default.
• W3048554174 cites W2148598721 @default.
• W3048554174 cites W2163312887 @default.
• W3048554174 cites W2171191165 @default.
• W3048554174 cites W2201997235 @default.
• W3048554174 cites W2206047037 @default.
• W3048554174 cites W2262373862 @default.
• W3048554174 cites W2300457014 @default.
• W3048554174 cites W2402185838 @default.
• W3048554174 cites W2473367284 @default.
• W3048554174 cites W2564407574 @default.
• W3048554174 cites W2808911211 @default.
• W3048554174 cites W2809961345 @default.
• W3048554174 cites W2890788691 @default.
• W3048554174 cites W2946148356 @default.
• W3048554174 doi "https://doi.org/10.1021/acschemneuro.0c00320" @default.
• W3048554174 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32786326" @default.
• W3048554174 hasPublicationYear "2020" @default.
• W3048554174 type Work @default.
• W3048554174 sameAs 3048554174 @default.
• W3048554174 citedByCount "1" @default.
• W3048554174 countsByYear W30485541742022 @default.
• W3048554174 crossrefType "journal-article" @default.
• W3048554174 hasAuthorship W3048554174A5019015682 @default.
• W3048554174 hasAuthorship W3048554174A5041256217 @default.
• W3048554174 hasAuthorship W3048554174A5047202581 @default.
• W3048554174 hasAuthorship W3048554174A5058773225 @default.
• W3048554174 hasConcept C104317684 @default.
• W3048554174 hasConcept C116569031 @default.
• W3048554174 hasConcept C12554922 @default.
• W3048554174 hasConcept C14036430 @default.
• W3048554174 hasConcept C143065580 @default.
• W3048554174 hasConcept C170493617 @default.
• W3048554174 hasConcept C185592680 @default.
• W3048554174 hasConcept C2779856020 @default.
• W3048554174 hasConcept C28406088 @default.
• W3048554174 hasConcept C50254741 @default.
• W3048554174 hasConcept C515207424 @default.
• W3048554174 hasConcept C55493867 @default.
• W3048554174 hasConcept C82617044 @default.
• W3048554174 hasConcept C86803240 @default.
• W3048554174 hasConcept C95444343 @default.
• W3048554174 hasConceptScore W3048554174C104317684 @default.
• W3048554174 hasConceptScore W3048554174C116569031 @default.
• W3048554174 hasConceptScore W3048554174C12554922 @default.
• W3048554174 hasConceptScore W3048554174C14036430 @default.
• W3048554174 hasConceptScore W3048554174C143065580 @default.
• W3048554174 hasConceptScore W3048554174C170493617 @default.
• W3048554174 hasConceptScore W3048554174C185592680 @default.
• W3048554174 hasConceptScore W3048554174C2779856020 @default.
• W3048554174 hasConceptScore W3048554174C28406088 @default.
• W3048554174 hasConceptScore W3048554174C50254741 @default.
• W3048554174 hasConceptScore W3048554174C515207424 @default.
• W3048554174 hasConceptScore W3048554174C55493867 @default.
• W3048554174 hasConceptScore W3048554174C82617044 @default.
• W3048554174 hasConceptScore W3048554174C86803240 @default.
• W3048554174 hasConceptScore W3048554174C95444343 @default.
• W3048554174 hasFunder F4320307770 @default.
• W3048554174 hasIssue "17" @default.
• W3048554174 hasLocation W30485541741 @default.
• W3048554174 hasOpenAccess W3048554174 @default.
• W3048554174 hasPrimaryLocation W30485541741 @default.
• W3048554174 hasRelatedWork W1816636001 @default.
• W3048554174 hasRelatedWork W1990758898 @default.
• W3048554174 hasRelatedWork W2032038036 @default.
• W3048554174 hasRelatedWork W2044257351 @default.
• W3048554174 hasRelatedWork W2058685556 @default.
• W3048554174 hasRelatedWork W2100769672 @default.

• next