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- W3048558122 abstract "A novel actinomycete, designated strain TRM 66233 T , was isolated from Apocynum venetum L. collected from the Xinjiang Uygur Autonomous Region of China and characterized using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain TRM 66233 T with the genus Streptomyces . Strain TRM 66233 T showed a high similarity value to Streptomyces bikiniensis NRRL B-1049 T (98.07 %) based on the 16S rRNA gene phylogenetic tree. The whole-cell sugar pattern of TRM 66233 T consisted of glucose, galactose, mannose and ribose. The predominant menaquinones were MK-9(H 2 ), MK-9(H 6 ), MK-9(H 8 ) and MK-9(H 10 ). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and four unidentified lipids. The major fatty acids were iso -C 15 : 0 , anteiso -C 15 : 0 , iso -C 16 : 0 , C 16 : 0 and iso -C 17 : 0 . The G+C content of the DNA was 70.35 mol%. The DNA–DNA relatedness and average nucleotide identity values as well as evolutionary distances based on multilocus ( atp D, gyr B, rec A, rpo B and trp B) sequences between strain TRM 66233 T and closely related type strains were significantly lower than the recommended threshold values. The whole-genome average nucleotide identity and digital DNA–DNA hybridization values between strain TRM 66233 T and S. bikiniensis NRRL B-1049 T were 78.86 and 23.2 %, respectively. On the basis of evidence from this polyphasic study, strain TRM 66233 T should represent a novel species of the genus Streptomyces , for which the name Streptomyces apocyni sp. nov. is proposed. The type strain is TRM 66233 T (=CCTCC AA 2019056 T =LMG 31559 T )." @default.
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- W3048558122 date "2020-09-01" @default.
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- W3048558122 title "Streptomyces apocyni sp. nov., an endogenous actinomycete isolated from Apocynum venetum" @default.
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- W3048558122 doi "https://doi.org/10.1099/ijsem.0.004357" @default.
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