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- W3048560057 abstract "Deoxyribozymes (DNAzymes) are small, synthetic, single-stranded DNAs capable of catalyzing chemical reactions, including RNA ligation. Herein, we report a novel class of RNA ligase deoxyribozymes that utilize 5′-adenylated RNA (5′-AppRNA) as the donor substrate, mimicking the activated intermediates of protein-catalyzed RNA ligation. Four new DNAzymes were identified by in vitro selection from an N40 random DNA library and were shown to catalyze the intermolecular linear RNA-RNA ligation via the formation of a native 3′-5′-phosphodiester linkage. The catalytic activity is distinct from previously described RNA-ligating deoxyribozymes. Kinetic analyses revealed the optimal incubation conditions for high ligation yields and demonstrated a broad RNA substrate scope. Together with the smooth synthetic accessibility of 5′-adenylated RNAs, the new DNA enzymes are promising tools for the protein-free synthesis of long RNAs, for example containing precious modified nucleotides or fluorescent labels for biochemical and biophysical investigations." @default.
- W3048560057 created "2020-08-18" @default.
- W3048560057 creator A5021290270 @default.
- W3048560057 creator A5043160931 @default.
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- W3048560057 date "2020-08-11" @default.
- W3048560057 modified "2023-10-18" @default.
- W3048560057 title "New Deoxyribozymes for the Native Ligation of RNA" @default.
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- W3048560057 doi "https://doi.org/10.3390/molecules25163650" @default.
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