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- W3048585446 endingPage "108905" @default.
- W3048585446 startingPage "108905" @default.
- W3048585446 abstract "The vertebrate brain comprises a plethora of cell types connected by intertwined pathways. Optogenetics enriches the neuroscientific tool set for disentangling these neuronal circuits in a manner which exceeds the spatio-temporal precision of previously existing techniques. Technically, optogenetics can be divided in three types of optical and genetic combinations: (1) it is primarily understood as the manipulation of the activity of genetically modified cells (typically neurons) with light, i.e. optical actuators. (2) A second combination refers to visualizing the activity of genetically modified cells (again typically neurons), i.e. optical sensors. (3) A completely different interpretation of optogenetics refers to the light activated expression of a genetically induced construct. Here, we focus on the first two types of optogenetics, i.e. the optical actuators and sensors in an attempt to give an overview into the topic. We first cover methods to express opsins into neurons and introduce strategies of targeting specific neuronal populations in different animal species. We then summarize combinations of optogenetics with behavioral read out and neuronal imaging. Finally, we give an overview of the current state-of-the-art and an outlook on future perspectives." @default.
- W3048585446 created "2020-08-18" @default.
- W3048585446 creator A5017967974 @default.
- W3048585446 creator A5065741892 @default.
- W3048585446 creator A5089185725 @default.
- W3048585446 date "2020-11-01" @default.
- W3048585446 modified "2023-10-04" @default.
- W3048585446 title "Functional interrogation of neural circuits with virally transmitted optogenetic tools" @default.
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