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• W3048589596 abstract "Aspergillus fumigatus is one of the most common airborne molds capable of causing mycoses and allergies in humans. During infection, fungal surface proteins mediate the first contact with the human immune system to evade immune responses or to induce hypersensitivity. Several methods have been established for surface proteomics (surfomics). Biotinylation coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification of peptides is a particularly efficient method to identify the surface-exposed regions of proteins that potentially mediate interaction with the host. After biotinylation of surface proteins during spore germination, we detected 231 different biotinylated surface proteins (including several well-known proteins such as RodA, CcpA, and DppV; allergens; and heat shock proteins [HSPs]), as well as some previously undescribed surface proteins. The dynamic change of the surface proteome was illustrated by detection of a relatively high number of proteins exclusively at one developmental stage. Using immunofluorescence microscopy, we confirmed the surface localization of several HSPs of the HSP70 family, which may have moonlighting functions. Collectively, by comparing our data with data representative of previously published A. fumigatus surface proteomes, our study generated a comprehensive data set corresponding to the A. fumigatus surfome and uncovered the surface-exposed regions of many proteins on the surface of conidia or hyphae. These surface-exposed regions are candidates for direct interaction with host cells and may represent antigenic epitopes that either induce protective immune responses or mediate immune evasion. Thus, our data sets provided and compiled here represent reasonable immunotherapy and diagnostic targets for future investigations.IMPORTANCEAspergillus fumigatus is the most important airborne human-pathogenic mold, capable of causing both life-threatening invasive pulmonary aspergillosis in immunocompromised patients and allergy-inducing infections in individuals with atopic allergy. Despite its obvious medical relevance, timely diagnosis and efficient antifungal treatment of A. fumigatus infection remain major challenges. Proteins on the surface of conidia (asexually produced spores) and mycelium directly mediate host-pathogen interaction and also may serve as targets for diagnosis and immunotherapy. However, the similarity of protein sequences between A. fumigatus and other organisms, sometimes even including the human host, makes selection of targets for immunological-based studies difficult. Here, using surface protein biotinylation coupled with LC-MS/MS analysis, we identified hundreds of A. fumigatus surface proteins with exposed regions, further defining putative targets for possible diagnostic and immunotherapeutic design." @default.
• W3048589596 created "2020-08-18" @default.
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• W3048589596 date "2020-08-26" @default.
• W3048589596 modified "2023-09-26" @default.
• W3048589596 title "Biotinylated Surfome Profiling Identifies Potential Biomarkers for Diagnosis and Therapy of Aspergillus fumigatus Infection" @default.
• W3048589596 cites W1527548373 @default.
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• W3048589596 cites W1842514681 @default.
• W3048589596 cites W1968000268 @default.
• W3048589596 cites W1984118541 @default.
• W3048589596 cites W2006986579 @default.
• W3048589596 cites W2013292266 @default.
• W3048589596 cites W2027656040 @default.
• W3048589596 cites W2028074506 @default.
• W3048589596 cites W2036903343 @default.
• W3048589596 cites W2042775525 @default.
• W3048589596 cites W2049518174 @default.
• W3048589596 cites W2050252824 @default.
• W3048589596 cites W2054537975 @default.
• W3048589596 cites W2063951356 @default.
• W3048589596 cites W2069204924 @default.
• W3048589596 cites W2069655320 @default.
• W3048589596 cites W2075736183 @default.
• W3048589596 cites W2077155024 @default.
• W3048589596 cites W2079928619 @default.
• W3048589596 cites W2090227081 @default.
• W3048589596 cites W2095779036 @default.
• W3048589596 cites W2095902922 @default.
• W3048589596 cites W2096209592 @default.
• W3048589596 cites W2099176294 @default.
• W3048589596 cites W2099224272 @default.
• W3048589596 cites W2099317703 @default.
• W3048589596 cites W2102419220 @default.
• W3048589596 cites W2102731598 @default.
• W3048589596 cites W2109847239 @default.
• W3048589596 cites W2114162558 @default.
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• W3048589596 cites W2137975942 @default.
• W3048589596 cites W2139627695 @default.
• W3048589596 cites W2143691385 @default.
• W3048589596 cites W2147406619 @default.
• W3048589596 cites W2148896980 @default.
• W3048589596 cites W2149051930 @default.
• W3048589596 cites W2151417485 @default.
• W3048589596 cites W2171120381 @default.
• W3048589596 cites W2171777893 @default.
• W3048589596 cites W2225487457 @default.
• W3048589596 cites W2233541949 @default.
• W3048589596 cites W2324262342 @default.
• W3048589596 cites W2338771957 @default.
• W3048589596 cites W2437250834 @default.
• W3048589596 cites W2520706740 @default.
• W3048589596 cites W2521634197 @default.
• W3048589596 cites W2551648587 @default.
• W3048589596 cites W2569440837 @default.
• W3048589596 cites W2775203471 @default.
• W3048589596 cites W2789652031 @default.
• W3048589596 cites W2790546589 @default.
• W3048589596 cites W2807119034 @default.
• W3048589596 cites W2818429755 @default.
• W3048589596 cites W2894964423 @default.
• W3048589596 cites W2899760200 @default.
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• W3048589596 doi "https://doi.org/10.1128/msphere.00535-20" @default.
• W3048589596 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7426169" @default.
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• W3048589596 hasPublicationYear "2020" @default.
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