Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048606661> ?p ?o ?g. }
- W3048606661 endingPage "1036" @default.
- W3048606661 startingPage "1036" @default.
- W3048606661 abstract "Leishmania infantum causes visceral leishmaniasis (kala-azar), the most severe form of leishmaniasis, which is lethal if untreated. A few years ago, the re-sequencing and de novo assembling of the L. infantum (JPCM5 strain) genome was accomplished, and now we aimed to describe and characterize the experimental proteome of this species. In this work, we performed a proteomic analysis from axenic cultured promastigotes and carried out a detailed comparison with other Leishmania experimental proteomes published to date. We identified 2352 proteins based on a search of mass spectrometry data against a database built from the six-frame translated genome sequence of L. infantum. We detected many proteins belonging to organelles such as glycosomes, mitochondria, or flagellum, as well as many metabolic enzymes and many putative RNA binding proteins and molecular chaperones. Moreover, we listed some proteins presenting post-translational modifications, such as phosphorylations, acetylations, and methylations. On the other hand, the identification of peptides mapping to genomic regions previously annotated as non-coding allowed for the correction of annotations, leading to the N-terminal extension of protein sequences and the uncovering of eight novel protein-coding genes. The alliance of proteomics, genomics, and transcriptomics has resulted in a powerful combination for improving the annotation of the L. infantum reference genome." @default.
- W3048606661 created "2020-08-18" @default.
- W3048606661 creator A5006113461 @default.
- W3048606661 creator A5025257753 @default.
- W3048606661 creator A5032052287 @default.
- W3048606661 creator A5041009516 @default.
- W3048606661 creator A5048071213 @default.
- W3048606661 creator A5056195591 @default.
- W3048606661 creator A5072600261 @default.
- W3048606661 creator A5086790393 @default.
- W3048606661 date "2020-09-02" @default.
- W3048606661 modified "2023-10-05" @default.
- W3048606661 title "The Experimental Proteome of Leishmania infantum Promastigote and Its Usefulness for Improving Gene Annotations" @default.
- W3048606661 cites W1513777552 @default.
- W3048606661 cites W1943769738 @default.
- W3048606661 cites W1958773130 @default.
- W3048606661 cites W1967609695 @default.
- W3048606661 cites W1967790997 @default.
- W3048606661 cites W1969686592 @default.
- W3048606661 cites W1970022594 @default.
- W3048606661 cites W1971315932 @default.
- W3048606661 cites W1973500293 @default.
- W3048606661 cites W1979920328 @default.
- W3048606661 cites W1980727585 @default.
- W3048606661 cites W1981764865 @default.
- W3048606661 cites W1988510768 @default.
- W3048606661 cites W1991923259 @default.
- W3048606661 cites W1994226299 @default.
- W3048606661 cites W1996146053 @default.
- W3048606661 cites W2001336779 @default.
- W3048606661 cites W2003783312 @default.
- W3048606661 cites W2004182243 @default.
- W3048606661 cites W2007905920 @default.
- W3048606661 cites W2010211659 @default.
- W3048606661 cites W2014472487 @default.
- W3048606661 cites W2019355435 @default.
- W3048606661 cites W2030272562 @default.
- W3048606661 cites W2037284663 @default.
- W3048606661 cites W2044731117 @default.
- W3048606661 cites W2059878803 @default.
- W3048606661 cites W2072857237 @default.
- W3048606661 cites W2078594804 @default.
- W3048606661 cites W2085352255 @default.
- W3048606661 cites W2106909352 @default.
- W3048606661 cites W2119845886 @default.
- W3048606661 cites W2120987638 @default.
- W3048606661 cites W2129223498 @default.
- W3048606661 cites W2135078307 @default.
- W3048606661 cites W2135790409 @default.
- W3048606661 cites W2144120140 @default.
- W3048606661 cites W2145859719 @default.
- W3048606661 cites W2146188580 @default.
- W3048606661 cites W2150328121 @default.
- W3048606661 cites W2166459128 @default.
- W3048606661 cites W2168799765 @default.
- W3048606661 cites W2169230579 @default.
- W3048606661 cites W2170302951 @default.
- W3048606661 cites W2186123618 @default.
- W3048606661 cites W2258656762 @default.
- W3048606661 cites W2283978559 @default.
- W3048606661 cites W2311753124 @default.
- W3048606661 cites W2315617380 @default.
- W3048606661 cites W2317321814 @default.
- W3048606661 cites W2326414500 @default.
- W3048606661 cites W2337282573 @default.
- W3048606661 cites W2378703660 @default.
- W3048606661 cites W2473711714 @default.
- W3048606661 cites W2478575915 @default.
- W3048606661 cites W2582865571 @default.
- W3048606661 cites W2592523451 @default.
- W3048606661 cites W2607462893 @default.
- W3048606661 cites W2778943499 @default.
- W3048606661 cites W2782830751 @default.
- W3048606661 cites W2797219803 @default.
- W3048606661 cites W2810225090 @default.
- W3048606661 cites W2891617613 @default.
- W3048606661 cites W2899604651 @default.
- W3048606661 cites W2905526301 @default.
- W3048606661 cites W2911081160 @default.
- W3048606661 cites W2945821925 @default.
- W3048606661 cites W2954523347 @default.
- W3048606661 cites W2966622402 @default.
- W3048606661 cites W2976530593 @default.
- W3048606661 cites W3014667265 @default.
- W3048606661 cites W305040560 @default.
- W3048606661 cites W320946909 @default.
- W3048606661 doi "https://doi.org/10.3390/genes11091036" @default.
- W3048606661 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7563732" @default.
- W3048606661 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32887454" @default.
- W3048606661 hasPublicationYear "2020" @default.
- W3048606661 type Work @default.
- W3048606661 sameAs 3048606661 @default.
- W3048606661 citedByCount "10" @default.
- W3048606661 countsByYear W30486066612020 @default.
- W3048606661 countsByYear W30486066612021 @default.
- W3048606661 countsByYear W30486066612022 @default.
- W3048606661 countsByYear W30486066612023 @default.
- W3048606661 crossrefType "journal-article" @default.