FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048609634> ?p ?o ?g. }

• W3048609634 endingPage "15370" @default.
• W3048609634 startingPage "15362" @default.
• W3048609634 abstract "EPR and Electron Nuclear Double Resonance spectroscopies here characterize CO binding to the active-site A cluster of wild-type (WT) Acetyl-CoA Synthase (ACS) and two variants, F229W and F229A. The A-cluster binds CO to a proximal Ni (Nip) that bridges a [4Fe-4S] cluster and a distal Nid. An alcove seen in the ACS crystal structure near the A-cluster, defined by hydrophobic residues including F229, forms a cage surrounding a Xe mimic of CO. Previously, we only knew WT ACS bound a single CO to form the Ared-CO intermediate, containing Nip(I)-CO with CO located on the axis of the dz2 odd-electron orbital (g⊥ > g|| ∼ 2). Here, the two-dimensional field-frequency pattern of 2K-35 GHz 13C-ENDOR spectra collected across the Ared-CO EPR envelope reveals a second CO bound in the dz2 orbital’s equatorial plane. This WT A-cluster conformer dominates the nearly conservative F229W variant, but 13C-ENDOR reveals a minority “A” conformation with (g|| > g⊥ ∼ 2) characteristic of a “cloverleaf” (e.g., dx2-y2) odd-electron orbital, with Nip binding two, apparently “in-plane” CO. Disruption of the alcove through introduction of the smaller alanine residue in the F229A variant diminishes conversion to Ni(I) ∼ 10-fold and introduces extensive cluster flexibility. 13C-ENDOR shows the F229A cluster is mostly (60%) in the “A” conformation but with ∼20% each of the WT conformer and an “O” state in which dz2 Nip(I) (g⊥ > g|| ∼ 2) surprisingly lacks CO. This paper thus demonstrates the importance of an intact alcove in forming and stabilizing the Ni(I)-CO intermediate in the Wood-Ljungdahl pathway of anaerobic CO and CO2 fixation." @default.
• W3048609634 created "2020-08-18" @default.
• W3048609634 creator A5034474295 @default.
• W3048609634 creator A5056583971 @default.
• W3048609634 creator A5080730574 @default.
• W3048609634 creator A5089695755 @default.
• W3048609634 date "2020-08-12" @default.
• W3048609634 modified "2023-09-26" @default.
• W3048609634 title "¹³C Electron Nuclear Double Resonance Spectroscopy Shows Acetyl-CoA Synthase Binds Two Substrate CO in Multiple Binding Modes and Reveals the Importance of a CO-Binding “Alcove”" @default.
• W3048609634 cites W1146692588 @default.
• W3048609634 cites W1490730015 @default.
• W3048609634 cites W1540990666 @default.
• W3048609634 cites W1586734181 @default.
• W3048609634 cites W1819850013 @default.
• W3048609634 cites W1964046434 @default.
• W3048609634 cites W1965187023 @default.
• W3048609634 cites W1972381640 @default.
• W3048609634 cites W1974520711 @default.
• W3048609634 cites W1977319410 @default.
• W3048609634 cites W1981887431 @default.
• W3048609634 cites W1984777731 @default.
• W3048609634 cites W1991948671 @default.
• W3048609634 cites W1993929532 @default.
• W3048609634 cites W2010044705 @default.
• W3048609634 cites W2011034421 @default.
• W3048609634 cites W2015672631 @default.
• W3048609634 cites W2019528254 @default.
• W3048609634 cites W2028371684 @default.
• W3048609634 cites W2040665477 @default.
• W3048609634 cites W2048516944 @default.
• W3048609634 cites W2059761417 @default.
• W3048609634 cites W2060998824 @default.
• W3048609634 cites W2061939602 @default.
• W3048609634 cites W2069214890 @default.
• W3048609634 cites W2070563230 @default.
• W3048609634 cites W2079448948 @default.
• W3048609634 cites W2088440437 @default.
• W3048609634 cites W2141066579 @default.
• W3048609634 cites W2146051607 @default.
• W3048609634 cites W2161674646 @default.
• W3048609634 cites W2273329620 @default.
• W3048609634 cites W2317044479 @default.
• W3048609634 cites W2587604825 @default.
• W3048609634 cites W2729425227 @default.
• W3048609634 cites W2900489966 @default.
• W3048609634 cites W2915492815 @default.
• W3048609634 cites W4235583278 @default.
• W3048609634 doi "https://doi.org/10.1021/jacs.0c05950" @default.
• W3048609634 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7781152" @default.
• W3048609634 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32786751" @default.
• W3048609634 hasPublicationYear "2020" @default.
• W3048609634 type Work @default.
• W3048609634 sameAs 3048609634 @default.
• W3048609634 citedByCount "9" @default.
• W3048609634 countsByYear W30486096342021 @default.
• W3048609634 countsByYear W30486096342022 @default.
• W3048609634 countsByYear W30486096342023 @default.
• W3048609634 crossrefType "journal-article" @default.
• W3048609634 hasAuthorship W3048609634A5034474295 @default.
• W3048609634 hasAuthorship W3048609634A5056583971 @default.
• W3048609634 hasAuthorship W3048609634A5080730574 @default.
• W3048609634 hasAuthorship W3048609634A5089695755 @default.
• W3048609634 hasBestOaLocation W30486096342 @default.
• W3048609634 hasConcept C121332964 @default.
• W3048609634 hasConcept C139210041 @default.
• W3048609634 hasConcept C164866538 @default.
• W3048609634 hasConcept C178790620 @default.
• W3048609634 hasConcept C184779094 @default.
• W3048609634 hasConcept C185592680 @default.
• W3048609634 hasConcept C18705241 @default.
• W3048609634 hasConcept C187961010 @default.
• W3048609634 hasConcept C199360897 @default.
• W3048609634 hasConcept C32909587 @default.
• W3048609634 hasConcept C41008148 @default.
• W3048609634 hasConcept C46141821 @default.
• W3048609634 hasConcept C65154329 @default.
• W3048609634 hasConcept C71240020 @default.
• W3048609634 hasConcept C8010536 @default.
• W3048609634 hasConceptScore W3048609634C121332964 @default.
• W3048609634 hasConceptScore W3048609634C139210041 @default.
• W3048609634 hasConceptScore W3048609634C164866538 @default.
• W3048609634 hasConceptScore W3048609634C178790620 @default.
• W3048609634 hasConceptScore W3048609634C184779094 @default.
• W3048609634 hasConceptScore W3048609634C185592680 @default.
• W3048609634 hasConceptScore W3048609634C18705241 @default.
• W3048609634 hasConceptScore W3048609634C187961010 @default.
• W3048609634 hasConceptScore W3048609634C199360897 @default.
• W3048609634 hasConceptScore W3048609634C32909587 @default.
• W3048609634 hasConceptScore W3048609634C41008148 @default.
• W3048609634 hasConceptScore W3048609634C46141821 @default.
• W3048609634 hasConceptScore W3048609634C65154329 @default.
• W3048609634 hasConceptScore W3048609634C71240020 @default.
• W3048609634 hasConceptScore W3048609634C8010536 @default.
• W3048609634 hasFunder F4320306076 @default.
• W3048609634 hasFunder F4320332161 @default.
• W3048609634 hasIssue "36" @default.
• W3048609634 hasLocation W30486096341 @default.
• W3048609634 hasLocation W30486096342 @default.

• next