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- W3048627982 abstract "The fission yeast Schizosaccharomyces pombe is an excellent model organism for the study of eukaryotic cellular physiology. The organism is genetically tractable and several tools to study the functions of individual genes are available. One such tool is regulatable gene expression and overproduction of proteins. Limitations of currently available overexpression systems include delay in expression after induction, narrow dynamic range, and system-wide changes due to induction conditions. Here I describe a new long noncoding RNA (lncRNA)-regulated, thiamine-inducible expression system that integrates lncRNA-based transcriptional interference at the fission yeast tgp1 promoter with the fast repression kinetics of the thiamine-repressible nmt1 promoter. This hybrid system has rapid induction kinetics, broad dynamic range, and tunable expression via thiamine concentration. The lncRNA-regulated thiamine-inducible system will be advantageous for the study of individual genes and for potential applications in the production of heterologous proteins in fission yeast." @default.
- W3048627982 created "2020-08-18" @default.
- W3048627982 creator A5080148743 @default.
- W3048627982 date "2020-08-11" @default.
- W3048627982 modified "2023-10-17" @default.
- W3048627982 title "A lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeastSchizosaccharomyces pombe" @default.
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- W3048627982 doi "https://doi.org/10.1261/rna.076000.120" @default.
- W3048627982 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7566572" @default.
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