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• W3048682419 abstract "Purpose. To screen and diagnose common mushroom (Agaricus bisporus (J. Lge) Imbach) for infection with mycoplasmas to obtain mushroom spawn free of infection. Methods. Visual, microbiological (obtaining pure fungus culture, studying cultural properties of colonies, determining sensitivity to antibiotics), electron microscopy, statistical. The studies were conducted during 2015–2019 at the Department of Ecobiotechnology and Biodiversity of the National University of Life and Environmental Sciences of Ukraine. There were studied spawn (strain Sylvan A-15, included in the State Register of Ukraine), casing material, substrate, and fruit bodies of common mushroom (40 samples) selected in mushroom farms in Vasylkiv and Kyiv-Sviatoshyn districts of Kiev region. Results. In 17 samples out of 40, symptoms of mycoplasma diseases were found. The symptoms inherent to them were diagnosed: acquisition of gray coloring by young fruit bodies, premature cap opening. Occurrence of exudates in the samples which was observed in 36% of the studied samples also indicates mycoplasmal nature. In the case of infection of casing material, the symptoms coincided with the data on the isolation and identification of the disease. The electron diffraction pattern confirms the presence of mycoplasma conglomerate in the deep spawn of common mushroom. Mycogone and mummification samples appeared to be highly sensitive to antibiotics (the sterile area around indicator disc with the antibiotic was 29 mm), Сoprinus and bacterial rust pathogen were the sensitive ones (19 mm), and bacterial lesion of substrate was the sensitive one (12 mm). The method for diagnosing mycoplasmas on fungi of the species A. bisporus has been improved for specific symptoms: necrosis and exudate. Conclusions. Primary screening of common mushroom samples in greenhouse conditions was performed using visual method and electron microscopy to diagnose mycoplasma infection of fruit bodies and spawn. Microbiological methods for the detection of mycoplasma infections in mushroom culture have been optimized." @default.
• W3048682419 created "2020-08-18" @default.
• W3048682419 creator A5088732769 @default.
• W3048682419 date "2019-06-15" @default.
• W3048682419 modified "2023-09-25" @default.
• W3048682419 title "Diagnosis of mycoplasmal infections on common mushroom in artificial agroecosystems" @default.
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• W3048682419 doi "https://doi.org/10.31073/mvis201908-05" @default.
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