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- W3048691978 abstract "Abstract This work presents the first use of yeast-displayed protein targets for screening mRNA display libraries of cyclic and linear peptides. The WW domains of Yes-Associated Protein 1 (WW-YAP) and mitochondrial import receptor subunit TOM22 were adopted as protein targets. Yeast cells displaying WW-YAP or TOM22 were magnetized with iron oxide nanoparticles to enable the isolation of target-binding mRNA-peptide fusions. Equilibrium adsorption studies were conducted to estimate the binding affinity (K D ) of selected WW-YAP-binding peptides: K D values of 37 μM and 4 μM were obtained for cyclo[M-AFRLC-K] and its linear cognate, and 40 μM and 3 μM for cyclo[M-LDFVNHRSRG-K] and its linear cognate, respectively. TOM22-binding peptide cyclo[M-PELNRAI-K] was conjugated to magnetic beads and incubated with yeast cells expressing TOM22 and luciferase. A luciferase-based assay showed a 4.5-fold higher binding of TOM22 + yeast compared to control cells. This work demonstrates that integrating mRNA and yeast display accelerates the discovery of biospecific peptides." @default.
- W3048691978 created "2020-08-18" @default.
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- W3048691978 date "2020-08-14" @default.
- W3048691978 modified "2023-10-16" @default.
- W3048691978 title "Use of target-displaying magnetized yeast in screening mRNA display peptide libraries to identify ligands" @default.
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- W3048691978 doi "https://doi.org/10.1101/2020.08.13.249722" @default.
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