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- W3048707640 abstract "ABSTRACT The basal transcription factors of cellular RNA polymerases (RNAPs) stimulate the initial RNA synthesis via poorly understood mechanisms. Here, we explored the mechanism employed by the bacterial factor σ in promoter-independent initial transcription. We found that the RNAP holoenzyme lacking the promoter-binding domain σ4 is ineffective in de novo transcription initiation and displays high propensity to pausing upon extension of RNAs 3 to 7 nucleotides in length. The σ4 domain stabilizes short RNA:DNA hybrids and suppresses pausing by stimulating RNAP active-center translocation. The anti-pausing activity of σ4 is modulated by its interaction with the β subunit flap domain and by the σ remodeling factors AsiA and RbpA. Our results suggest that the presence of σ4 within the RNA exit channel compensates for the intrinsic instability of short RNA:DNA hybrids by increasing RNAP processivity, thus favoring productive transcription initiation. This “RNAP boosting” activity of the initiation factor is shaped by the the thermodynamics of RNA:DNA interactions and thus, should be relevant for any factor-dependent RNAP." @default.
- W3048707640 created "2020-08-18" @default.
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- W3048707640 date "2020-08-11" @default.
- W3048707640 modified "2023-09-25" @default.
- W3048707640 title "Anti-pausing activity of region 4 of the RNA polymerase σ subunit and its regulation by σ-remodeling factors" @default.
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- W3048707640 doi "https://doi.org/10.1101/2020.08.11.244855" @default.
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