FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048771804> ?p ?o ?g. }

• W3048771804 endingPage "103" @default.
• W3048771804 startingPage "87" @default.
• W3048771804 abstract "The blood-brain barrier (BBB) constituted by endothelial cells of brain microvessels is a dynamic interface, which controls and regulates the transport of various substances including peptides, proteins, ions, vitamins, hormones, and immune cells from the circulation into the brain parenchyma. Certain diseases/disorders such as Alzheimer's disease, sepsis, and hypertension can lead to varying degrees of BBB disruption. Moreover, impairment of BBB integrity has been implicated in the pathogenesis of various neurodegenerative diseases like epilepsy. In attempts to explore the wide spectrum of pathophysiologic mechanisms of these diseases/disorders, a variety of experimental insults targeted to the BBB integrity in vitro in cell culture models and in vivo in laboratory animals have been shown to alter BBB permeability causing enhanced transport of certain tracers such as sodium fluorescein, cadaverine-Alexa fluor, horseradish peroxidase, FITC-dextran, albumin-Alexa fluor conjugates, and Evans blue dye across the barrier. The permeability changes in barrier-type endothelial cells can be assessed by intravascular infusion of exogenous tracers and subsequent detection of the extravasated tracer in the brain tissue, which enable functional and structural analysis of BBB integrity. In this chapter, we aimed to highlight the current knowledge on the use of four most commonly performed tracers, namely, Evans blue, sodium fluorescein, albumin-Alexa fluor conjugates, and horseradish peroxidase. The experimental methodologies that we use in our laboratory for the detection of these tracers by macroscopy, spectrophotometry, spectrophotofluorometry, confocal laser scanning microscopy, and electron microscopy are also discussed. Tracing studies at the morphological level are mainly aimed at the identification of the tracers both in the barrier-related cells and brain parenchyma. In addition, BBB permeability to the tracers can be quantified using spectrophotometric and spectrophotofluorometric assays and image analysis by confocal laser scanning microscopy and electron microscopy. The results of our studies conducted under various experimental settings using the mentioned tracers indicate that barrier-type endothelial cells in brain microvessels orchestrate the paracellular and/or transcellular trafficking of substances across BBB. These efforts may not only contribute to designing approaches for the management of diseases/disorders associated with BBB breakdown but may also provide new insights for developing novel brain drug delivery strategies." @default.
• W3048771804 created "2020-08-18" @default.
• W3048771804 creator A5039614451 @default.
• W3048771804 creator A5055710898 @default.
• W3048771804 date "2020-01-01" @default.
• W3048771804 modified "2023-10-06" @default.
• W3048771804 title "Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase" @default.
• W3048771804 cites W1571481062 @default.
• W3048771804 cites W1852676216 @default.
• W3048771804 cites W1966629642 @default.
• W3048771804 cites W1972908695 @default.
• W3048771804 cites W1977940746 @default.
• W3048771804 cites W1987727899 @default.
• W3048771804 cites W1993191156 @default.
• W3048771804 cites W1994125288 @default.
• W3048771804 cites W1997575011 @default.
• W3048771804 cites W1998620774 @default.
• W3048771804 cites W2000441131 @default.
• W3048771804 cites W2003578465 @default.
• W3048771804 cites W2004613789 @default.
• W3048771804 cites W2008272454 @default.
• W3048771804 cites W2008625318 @default.
• W3048771804 cites W2015541518 @default.
• W3048771804 cites W2016471165 @default.
• W3048771804 cites W2020530488 @default.
• W3048771804 cites W2021314664 @default.
• W3048771804 cites W2022779476 @default.
• W3048771804 cites W2026078285 @default.
• W3048771804 cites W2028336428 @default.
• W3048771804 cites W2035833975 @default.
• W3048771804 cites W2035950831 @default.
• W3048771804 cites W2038613957 @default.
• W3048771804 cites W2041548571 @default.
• W3048771804 cites W2056856515 @default.
• W3048771804 cites W2069317492 @default.
• W3048771804 cites W2069604698 @default.
• W3048771804 cites W2070725864 @default.
• W3048771804 cites W2074782193 @default.
• W3048771804 cites W2084200097 @default.
• W3048771804 cites W2087478674 @default.
• W3048771804 cites W2087666006 @default.
• W3048771804 cites W2094827231 @default.
• W3048771804 cites W2098208110 @default.
• W3048771804 cites W2100951990 @default.
• W3048771804 cites W2101476558 @default.
• W3048771804 cites W2135426306 @default.
• W3048771804 cites W2148464749 @default.
• W3048771804 cites W2149295320 @default.
• W3048771804 cites W2152043216 @default.
• W3048771804 cites W2152355136 @default.
• W3048771804 cites W2159821105 @default.
• W3048771804 cites W2182660811 @default.
• W3048771804 cites W2278834524 @default.
• W3048771804 cites W2292758320 @default.
• W3048771804 cites W2314985889 @default.
• W3048771804 cites W2464443837 @default.
• W3048771804 cites W2560127228 @default.
• W3048771804 cites W2572176628 @default.
• W3048771804 cites W2619078508 @default.
• W3048771804 cites W2768931384 @default.
• W3048771804 cites W2884127226 @default.
• W3048771804 cites W2886121074 @default.
• W3048771804 cites W2890932781 @default.
• W3048771804 cites W2906400102 @default.
• W3048771804 cites W2913742117 @default.
• W3048771804 cites W2927523753 @default.
• W3048771804 cites W2938330154 @default.
• W3048771804 cites W2980690642 @default.
• W3048771804 cites W2984313823 @default.
• W3048771804 cites W2989756568 @default.
• W3048771804 cites W3005995871 @default.
• W3048771804 cites W3012458365 @default.
• W3048771804 doi "https://doi.org/10.1007/7651_2020_316" @default.
• W3048771804 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32785841" @default.
• W3048771804 hasPublicationYear "2020" @default.
• W3048771804 type Work @default.
• W3048771804 sameAs 3048771804 @default.
• W3048771804 citedByCount "25" @default.
• W3048771804 countsByYear W30487718042021 @default.
• W3048771804 countsByYear W30487718042022 @default.
• W3048771804 countsByYear W30487718042023 @default.
• W3048771804 crossrefType "book-chapter" @default.
• W3048771804 hasAuthorship W3048771804A5039614451 @default.
• W3048771804 hasAuthorship W3048771804A5055710898 @default.
• W3048771804 hasConcept C113045295 @default.
• W3048771804 hasConcept C121332964 @default.
• W3048771804 hasConcept C12554922 @default.
• W3048771804 hasConcept C134018914 @default.
• W3048771804 hasConcept C142724271 @default.
• W3048771804 hasConcept C169760540 @default.
• W3048771804 hasConcept C181199279 @default.
• W3048771804 hasConcept C185592680 @default.
• W3048771804 hasConcept C2776125364 @default.
• W3048771804 hasConcept C2776759294 @default.
• W3048771804 hasConcept C2777649898 @default.
• W3048771804 hasConcept C2777676563 @default.
• W3048771804 hasConcept C2778015030 @default.
• W3048771804 hasConcept C2778402981 @default.

• next