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• W3048817966 abstract "We sought to apply ribosome engineering (RE) to probiotic lactic acid bacteria and to verify RE’s impact. Here, we showed that one mutant of RE Lacticaseibacillus rhamnosus GG (LGG-MT K56N ) bore a GAPDH on the cell surface; the GAPDH was exported via an ABC transporter. Compared to the wild-type parent, LGG-MT K56N adhered more strongly to human colonic mucin and exhibited a distinct cell size and shape. These findings demonstrate that RE in LGG-MT K56N yielded dramatic changes in protein synthesis, protein transport, and cell morphology and affected adherence to human colonic mucin." @default.
• W3048817966 created "2020-08-18" @default.
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• W3048817966 date "2020-10-01" @default.
• W3048817966 modified "2023-10-17" @default.
• W3048817966 title "Ribosome-Engineered <i>Lacticaseibacillus rhamnosus</i> Strain GG Exhibits Cell Surface Glyceraldehyde-3-Phosphate Dehydrogenase Accumulation and Enhanced Adhesion to Human Colonic Mucin" @default.
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• W3048817966 doi "https://doi.org/10.1128/aem.01448-20" @default.
• W3048817966 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7531950" @default.
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