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W3048875538 abstract "Enzyme-linked immunosorbent assay (ELISA) provides a convenient means for detection of furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ). However, conventional ELISA bears drawback of unsatisfactory sensitivity. Hence, development of high-performance ELISA is of great significance for food safety. We report a signal amplified ELISA strategy for sensitive detection of AMOZ by incorporating gold nanoparticles (AuNPs) into conventional ELISA. In this system, AMOZ competes with bovine serum albumin-AMOZ (BSA-AMOZ) to bind to limited amount of monoclonal antibody; and a large amount of reporter antibody and horseradish peroxidase molecules are loaded onto AuNPs to amplify colorimetric signals. Under the optimized conditions, the signal amplified ELISA achieves a limit of detection of 10−2 ng/mL, which is 500-fold improvement than traditional ELISA in sensitivity. Moreover, pork liver samples spiked with different amount of AMOZ are successfully measured, suggesting this signal amplified ELISA can be used as a general signal enhancement strategy for determination of low-molecular weight contaminants in various foods." @default.
W3048875538 created "2020-08-18" @default.
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W3048875538 date "2020-12-01" @default.
W3048875538 modified "2023-10-16" @default.
W3048875538 title "Signal amplified enzyme-linked immunosorbent assay with gold nanoparticles for sensitive detection of trace furaltadone metabolite" @default.
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W3048875538 doi "https://doi.org/10.1016/j.microc.2020.105414" @default.
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