FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048886586> ?p ?o ?g. }

• W3048886586 endingPage "100" @default.
• W3048886586 startingPage "89" @default.
• W3048886586 abstract "Explore the effect of GATA5 expression on Paclitaxel inhibiting growth of hepatocellular carcinoma (HCC) cells.In the experimental study, HCC cell lines (HLE, Bel7402 and PLC/PRF/5) were treated with different concentrations of Paclitaxel (5-20 mg/ml) for 24 hours. HLE cells were transfected with GATA5-siRNA vector, while Bel7402 and PLC/PRF/5 cells were transfected with overexpressed GATA5 vector for 24 hours, followed by treatment of the cells with Paclitaxel (10 mg/ml) for 24 hours and subsequently 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay to detect growth of HCC cells. Soft agar cultured was used to analyze formation of colony. Apoptosis of HCC cells were detected by Flow cytometer. Migration of HCC cells was observed by trawell assays. Western blotting and laser confocal microscopy were utilized to detect expression and location of the proteins.Inhibiting expression of GATA5 reduced sensitivity of HLE cells to Paclitaxel, while overexpression of GATA5 increased sensitivity of Bel7402 cells and PLC/PRF/5 cells to Paclitaxel. Overexpression of GATA5 played a role in stimulating Paclitaxel to inhibit growth, colony formation and migration, as well as enhance apoptosis in HCC cells. Overexpression of GATA5 also promoted Paclitaxel to inhibit expression of reprogramming genes, such as Nanog, EpCAM, c-Myc and Sox2 in Bel7402 and PLC/PRF/5 cells. Inhibited expression of GATA5 led to enhancement of the expression of CD44 and CD133, in HLE cells. Overexpression of GATA5 was not only alone but also synergized with Paclitaxel to inhibit expression of CD44 and CD133 in Bel7402 or PLC/PRF/5 cells.Overexpression of GATA5 played a role in enhancing Paclitaxel to inhibit the malignant behaviors of HCC cells. It was involved in suppressing expression of the reprogramming genes and stemness markers. Targeting GATA5 is an available strategy for applying paclitaxel to therapy of patients with HCC." @default.
• W3048886586 created "2020-08-18" @default.
• W3048886586 creator A5005741943 @default.
• W3048886586 creator A5009508147 @default.
• W3048886586 creator A5018939784 @default.
• W3048886586 creator A5028702097 @default.
• W3048886586 creator A5041024027 @default.
• W3048886586 creator A5049393970 @default.
• W3048886586 creator A5060552570 @default.
• W3048886586 creator A5067593763 @default.
• W3048886586 date "2020-07-01" @default.
• W3048886586 modified "2023-09-23" @default.
• W3048886586 title "Overexpression of GATA5 Stimulates Paclitaxel to Inhibit Malignant Behaviors of Hepatocellular Carcinoma Cells." @default.
• W3048886586 cites W1600881103 @default.
• W3048886586 cites W1861546069 @default.
• W3048886586 cites W1977934858 @default.
• W3048886586 cites W1999184204 @default.
• W3048886586 cites W2003938487 @default.
• W3048886586 cites W2011124160 @default.
• W3048886586 cites W2028103767 @default.
• W3048886586 cites W2033785278 @default.
• W3048886586 cites W2060219833 @default.
• W3048886586 cites W2069224110 @default.
• W3048886586 cites W2071246356 @default.
• W3048886586 cites W2077087490 @default.
• W3048886586 cites W2078193006 @default.
• W3048886586 cites W2092460191 @default.
• W3048886586 cites W2092613163 @default.
• W3048886586 cites W2094751839 @default.
• W3048886586 cites W2114996432 @default.
• W3048886586 cites W2123125812 @default.
• W3048886586 cites W2126697949 @default.
• W3048886586 cites W2130976070 @default.
• W3048886586 cites W2134456945 @default.
• W3048886586 cites W2173684590 @default.
• W3048886586 cites W2235252850 @default.
• W3048886586 cites W2414070433 @default.
• W3048886586 cites W2560186031 @default.
• W3048886586 cites W2590276694 @default.
• W3048886586 cites W2790053839 @default.
• W3048886586 cites W2895813892 @default.
• W3048886586 cites W2912249711 @default.
• W3048886586 cites W2917837889 @default.
• W3048886586 doi "https://doi.org/10.22074/cellj.2020.6894" @default.
• W3048886586 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7481888" @default.
• W3048886586 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32779438" @default.
• W3048886586 hasPublicationYear "2020" @default.
• W3048886586 type Work @default.
• W3048886586 sameAs 3048886586 @default.
• W3048886586 citedByCount "1" @default.
• W3048886586 countsByYear W30488865862023 @default.
• W3048886586 crossrefType "journal-article" @default.
• W3048886586 hasAuthorship W3048886586A5005741943 @default.
• W3048886586 hasAuthorship W3048886586A5009508147 @default.
• W3048886586 hasAuthorship W3048886586A5018939784 @default.
• W3048886586 hasAuthorship W3048886586A5028702097 @default.
• W3048886586 hasAuthorship W3048886586A5041024027 @default.
• W3048886586 hasAuthorship W3048886586A5049393970 @default.
• W3048886586 hasAuthorship W3048886586A5060552570 @default.
• W3048886586 hasAuthorship W3048886586A5067593763 @default.
• W3048886586 hasConcept C121608353 @default.
• W3048886586 hasConcept C1491633281 @default.
• W3048886586 hasConcept C153911025 @default.
• W3048886586 hasConcept C2777292972 @default.
• W3048886586 hasConcept C2780932548 @default.
• W3048886586 hasConcept C502942594 @default.
• W3048886586 hasConcept C54009773 @default.
• W3048886586 hasConcept C54355233 @default.
• W3048886586 hasConcept C55493867 @default.
• W3048886586 hasConcept C62112901 @default.
• W3048886586 hasConcept C81885089 @default.
• W3048886586 hasConcept C86803240 @default.
• W3048886586 hasConceptScore W3048886586C121608353 @default.
• W3048886586 hasConceptScore W3048886586C1491633281 @default.
• W3048886586 hasConceptScore W3048886586C153911025 @default.
• W3048886586 hasConceptScore W3048886586C2777292972 @default.
• W3048886586 hasConceptScore W3048886586C2780932548 @default.
• W3048886586 hasConceptScore W3048886586C502942594 @default.
• W3048886586 hasConceptScore W3048886586C54009773 @default.
• W3048886586 hasConceptScore W3048886586C54355233 @default.
• W3048886586 hasConceptScore W3048886586C55493867 @default.
• W3048886586 hasConceptScore W3048886586C62112901 @default.
• W3048886586 hasConceptScore W3048886586C81885089 @default.
• W3048886586 hasConceptScore W3048886586C86803240 @default.
• W3048886586 hasIssue "Suppl 1" @default.
• W3048886586 hasLocation W30488865861 @default.
• W3048886586 hasLocation W30488865862 @default.
• W3048886586 hasOpenAccess W3048886586 @default.
• W3048886586 hasPrimaryLocation W30488865861 @default.
• W3048886586 hasRelatedWork W2010930267 @default.
• W3048886586 hasRelatedWork W2057925004 @default.
• W3048886586 hasRelatedWork W2160379231 @default.
• W3048886586 hasRelatedWork W2351812146 @default.
• W3048886586 hasRelatedWork W2357914589 @default.
• W3048886586 hasRelatedWork W2358285435 @default.
• W3048886586 hasRelatedWork W2371441531 @default.
• W3048886586 hasRelatedWork W2377726572 @default.
• W3048886586 hasRelatedWork W2379763872 @default.

• next