FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3048941398> ?p ?o ?g. }

• W3048941398 abstract "Renal ischemia-reperfusion injury (IRI) is a major cause of acute kidney injury (AKI). As a transcription factor, the Transcript induced in spermiogenesis 40 (Tisp40) has been found to be involved in renal IRI. However, the role of Tisp40 in tubular epithelial cell (TEC) pyroptosis of renal IRI remains unknown. In this study, we investigated effects of Tisp40 on Gasdermin D (GSDMD)-mediated TEC pyroptosis in renal IRI and underlying molecular mechanisms in I/R-induced kidney by hematoxylin and eosin (HE) staining, Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay,immunohistochemistry (IHC), reverse transcription-quantitative PCR (RT-qPCR) and western blot analysis in vivo and oxygen-glucose deprivation/reoxygenation (OGD/R)-stimulated TCMK-1 cells by lactate dehydrogenase (LDH) release assay, CCK-8 assay,enzyme-linked immunosorbent assay (ELISA), flow cytometric analysis, immunofluorescence staining,RT-qPCRand western blot analysis in vitro. We found that the levels of Tisp40 and GSDMD-N expression increased gradually, and peaked at 30 min ischemia/24 h reperfusion in vivo and 24 h OGD/R/6 h reoxygenation in vitro, simultaneously, the levels of TEC pyroptosis and renal injury were correspondingly increased. The data of Pearson's correlation analysis showed that the expression of Tisp40 and GSDMD-N was positively correlated. Furthermore, Tisp40 overexpression aggravated TEC pyroptosis rate and increased the expressions of related proteins, including GSDMD-N, NLRP3, caspase-1, IL-1β, and IL-18 in the OGD/R-stimulated TCMK-1 cell line, whereas the opposite occurred in cells treated with small interfeing RNA (siRNA) targeting Tisp40. Tisp40-deficient mice showed attenuated renal IRI and pyroptosis compared with wild-type mice. In addition, Tisp40 knockout remarkably decreased the levels of GSDMD-N, IL-1β, IL-18, NLRP3, and caspase-1 expression, and alleviated renal pyroptosis induced by I/R. Importantly, Tisp40 overexpression significantly increased TECs pyroptosis via p-p65 activation, however, the effects of Tisp40 overexpression were partially blocked by parthenolide (PTL). Collectively, our findings provide insight into the mechanism of how Tisp40 regulated GSDMD-mediated pyroptosis in renal IRI." @default.
• W3048941398 created "2020-08-18" @default.
• W3048941398 creator A5001655311 @default.
• W3048941398 creator A5042570050 @default.
• W3048941398 creator A5043688152 @default.
• W3048941398 creator A5051322135 @default.
• W3048941398 creator A5056273491 @default.
• W3048941398 creator A5071730303 @default.
• W3048941398 creator A5078521996 @default.
• W3048941398 date "2020-08-13" @default.
• W3048941398 modified "2023-10-14" @default.
• W3048941398 title "Tisp40 Induces Tubular Epithelial Cell GSDMD-Mediated Pyroptosis in Renal Ischemia-Reperfusion Injury via NF-κB Signaling" @default.
• W3048941398 cites W1488316114 @default.
• W3048941398 cites W1561544256 @default.
• W3048941398 cites W1864325817 @default.
• W3048941398 cites W1917792242 @default.
• W3048941398 cites W2024651527 @default.
• W3048941398 cites W2054898931 @default.
• W3048941398 cites W2073189296 @default.
• W3048941398 cites W2077967941 @default.
• W3048941398 cites W2097283404 @default.
• W3048941398 cites W2097714951 @default.
• W3048941398 cites W2101800432 @default.
• W3048941398 cites W2107277218 @default.
• W3048941398 cites W2115148169 @default.
• W3048941398 cites W2154500525 @default.
• W3048941398 cites W2155088804 @default.
• W3048941398 cites W2163511724 @default.
• W3048941398 cites W2164249826 @default.
• W3048941398 cites W2166825401 @default.
• W3048941398 cites W2192080449 @default.
• W3048941398 cites W2220606307 @default.
• W3048941398 cites W2227575066 @default.
• W3048941398 cites W2304527713 @default.
• W3048941398 cites W2340429612 @default.
• W3048941398 cites W2462773201 @default.
• W3048941398 cites W2560541542 @default.
• W3048941398 cites W2582602949 @default.
• W3048941398 cites W2626443942 @default.
• W3048941398 cites W2627048017 @default.
• W3048941398 cites W2739535833 @default.
• W3048941398 cites W2885824188 @default.
• W3048941398 cites W2918568966 @default.
• W3048941398 cites W2969750300 @default.
• W3048941398 cites W2989676031 @default.
• W3048941398 cites W2989852004 @default.
• W3048941398 cites W654486216 @default.
• W3048941398 doi "https://doi.org/10.3389/fphys.2020.00906" @default.
• W3048941398 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7438479" @default.
• W3048941398 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32903383" @default.
• W3048941398 hasPublicationYear "2020" @default.
• W3048941398 type Work @default.
• W3048941398 sameAs 3048941398 @default.
• W3048941398 citedByCount "27" @default.
• W3048941398 countsByYear W30489413982021 @default.
• W3048941398 countsByYear W30489413982022 @default.
• W3048941398 countsByYear W30489413982023 @default.
• W3048941398 crossrefType "journal-article" @default.
• W3048941398 hasAuthorship W3048941398A5001655311 @default.
• W3048941398 hasAuthorship W3048941398A5042570050 @default.
• W3048941398 hasAuthorship W3048941398A5043688152 @default.
• W3048941398 hasAuthorship W3048941398A5051322135 @default.
• W3048941398 hasAuthorship W3048941398A5056273491 @default.
• W3048941398 hasAuthorship W3048941398A5071730303 @default.
• W3048941398 hasAuthorship W3048941398A5078521996 @default.
• W3048941398 hasBestOaLocation W30489413981 @default.
• W3048941398 hasConcept C104317684 @default.
• W3048941398 hasConcept C126322002 @default.
• W3048941398 hasConcept C134018914 @default.
• W3048941398 hasConcept C150903083 @default.
• W3048941398 hasConcept C153911025 @default.
• W3048941398 hasConcept C154113507 @default.
• W3048941398 hasConcept C16685009 @default.
• W3048941398 hasConcept C17172800 @default.
• W3048941398 hasConcept C181199279 @default.
• W3048941398 hasConcept C185592680 @default.
• W3048941398 hasConcept C190283241 @default.
• W3048941398 hasConcept C196795494 @default.
• W3048941398 hasConcept C207001950 @default.
• W3048941398 hasConcept C2776415932 @default.
• W3048941398 hasConcept C2777318727 @default.
• W3048941398 hasConcept C2780091579 @default.
• W3048941398 hasConcept C2780114680 @default.
• W3048941398 hasConcept C2780721119 @default.
• W3048941398 hasConcept C31573885 @default.
• W3048941398 hasConcept C541997718 @default.
• W3048941398 hasConcept C55493867 @default.
• W3048941398 hasConcept C71924100 @default.
• W3048941398 hasConcept C86803240 @default.
• W3048941398 hasConceptScore W3048941398C104317684 @default.
• W3048941398 hasConceptScore W3048941398C126322002 @default.
• W3048941398 hasConceptScore W3048941398C134018914 @default.
• W3048941398 hasConceptScore W3048941398C150903083 @default.
• W3048941398 hasConceptScore W3048941398C153911025 @default.
• W3048941398 hasConceptScore W3048941398C154113507 @default.
• W3048941398 hasConceptScore W3048941398C16685009 @default.
• W3048941398 hasConceptScore W3048941398C17172800 @default.
• W3048941398 hasConceptScore W3048941398C181199279 @default.
• W3048941398 hasConceptScore W3048941398C185592680 @default.
• W3048941398 hasConceptScore W3048941398C190283241 @default.

• next