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• W3048953400 endingPage "e0232806" @default.
• W3048953400 startingPage "e0232806" @default.
• W3048953400 abstract "There is an increasing consumer demand for minimally processed, preservative free and microbiologically safe food. These factors, combined with risks of antibiotic resistance, have led to interest in bacteriocins produced by lactic acid bacteria (LAB) as natural food preservatives and as potential protein therapeutics. We previously reported the discovery of plantacyclin B21AG, a circular bacteriocin produced by Lactobacillus plantarum B21. Here, we describe the cloning and functional expression of the bacteriocin gene cluster in the probiotic Lactobacillus plantarum WCFS1. Genome sequencing demonstrated that the bacteriocin is encoded on a 20 kb native plasmid, designated as pB21AG01. Seven open reading frames (ORFs) putatively involved in bacteriocin production, secretion and immunity were cloned into an E. coli/Lactobacillus shuttle vector, pTRKH2. The resulting plasmid, pCycB21, was transformed into L. plantarum WCFS1. The cell free supernatants (CFS) of both B21 and WCFS1 (pCycB21) showed an antimicrobial activity of 800 AU/mL when tested against WCFS1 (pTRKH2) as the indicator strain, showing that functional expression of plantacyclin B21AG had been achieved. Real-time PCR analysis revealed that the relative copy number of pB21AG01 was 7.60 ± 0.79 in L. plantarum B21 whilst pCycB21 and pTRKH2 was 0.51 ± 0.05 and 25.19 ± 2.68 copies respectively in WCFS1. This indicates that the bacteriocin gene cluster is located on a highly stable low copy number plasmid pB21AG01 in L. plantarum B21. Inclusion of the native promoter for the bacteriocin operon from pB21AG01 results in similar killing activity being observed in both the wild type and recombinant hosts despite the lower copy number of pCycB21." @default.
• W3048953400 created "2020-08-18" @default.
• W3048953400 creator A5011834580 @default.
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• W3048953400 creator A5080888431 @default.
• W3048953400 date "2020-08-12" @default.
• W3048953400 modified "2023-10-17" @default.
• W3048953400 title "Cloning and functional expression of a food-grade circular bacteriocin, plantacyclin B21AG, in probiotic Lactobacillus plantarum WCFS1" @default.
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• W3048953400 cites W1544835748 @default.
• W3048953400 cites W1580302244 @default.
• W3048953400 cites W1878073525 @default.
• W3048953400 cites W1949136023 @default.
• W3048953400 cites W1968232055 @default.
• W3048953400 cites W1970079859 @default.
• W3048953400 cites W1975665089 @default.
• W3048953400 cites W1978548095 @default.
• W3048953400 cites W1979963523 @default.
• W3048953400 cites W1981680575 @default.
• W3048953400 cites W1982508713 @default.
• W3048953400 cites W1983654993 @default.
• W3048953400 cites W1988876705 @default.
• W3048953400 cites W1998835867 @default.
• W3048953400 cites W1999652679 @default.
• W3048953400 cites W2003660859 @default.
• W3048953400 cites W2004548026 @default.
• W3048953400 cites W2007287243 @default.
• W3048953400 cites W2009221323 @default.
• W3048953400 cites W2012173993 @default.
• W3048953400 cites W2016052312 @default.
• W3048953400 cites W2023245980 @default.
• W3048953400 cites W2037701905 @default.
• W3048953400 cites W2042354429 @default.
• W3048953400 cites W2051612190 @default.
• W3048953400 cites W2057315622 @default.
• W3048953400 cites W2057632176 @default.
• W3048953400 cites W2071422363 @default.
• W3048953400 cites W2075929383 @default.
• W3048953400 cites W2081842978 @default.
• W3048953400 cites W2084492158 @default.
• W3048953400 cites W2087786264 @default.
• W3048953400 cites W2089705909 @default.
• W3048953400 cites W2090216269 @default.
• W3048953400 cites W2096299972 @default.
• W3048953400 cites W2097255982 @default.
• W3048953400 cites W2099487182 @default.
• W3048953400 cites W2102184190 @default.
• W3048953400 cites W2102298254 @default.
• W3048953400 cites W2102672175 @default.
• W3048953400 cites W2106418680 @default.
• W3048953400 cites W2106488688 @default.
• W3048953400 cites W2108346683 @default.
• W3048953400 cites W2110479072 @default.
• W3048953400 cites W2113796404 @default.
• W3048953400 cites W2114645323 @default.
• W3048953400 cites W2117316721 @default.
• W3048953400 cites W2117629729 @default.
• W3048953400 cites W2120167586 @default.
• W3048953400 cites W2126634586 @default.
• W3048953400 cites W2129933858 @default.
• W3048953400 cites W2131149574 @default.
• W3048953400 cites W2135337280 @default.
• W3048953400 cites W2139021858 @default.
• W3048953400 cites W2144610292 @default.
• W3048953400 cites W2145526497 @default.
• W3048953400 cites W2152770371 @default.
• W3048953400 cites W2153544371 @default.
• W3048953400 cites W2156319674 @default.
• W3048953400 cites W2156984042 @default.
• W3048953400 cites W2158714788 @default.
• W3048953400 cites W2159024313 @default.
• W3048953400 cites W2462148310 @default.
• W3048953400 cites W2591694823 @default.
• W3048953400 cites W2621018081 @default.
• W3048953400 cites W2763070324 @default.
• W3048953400 cites W2783981376 @default.
• W3048953400 cites W2785332302 @default.
• W3048953400 cites W2886540103 @default.
• W3048953400 cites W2891989846 @default.
• W3048953400 cites W3195808090 @default.
• W3048953400 cites W4233060568 @default.
• W3048953400 cites W4233243661 @default.
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• W3048953400 doi "https://doi.org/10.1371/journal.pone.0232806" @default.
• W3048953400 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7423119" @default.
• W3048953400 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32785265" @default.
• W3048953400 hasPublicationYear "2020" @default.
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