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• W3048963208 abstract "T cell activation depends from mitochondrial derived reactive oxygen species (mtROS). However, a thorough mechanistic knowledge of how and where mtROS acts in the signaling network of activated T cells is missing. This thesis aimed (i) to evaluate the mtROS signaling network in activated primary human T cells and (ii) to reveal the impact of mtROS signaling on immune phenotypes and effector functions. First, an mtROS inducible model based on primary human CD4+ T cells was established and probed with proteomics. By utilizing an isobaric thiol switching strategy, I performed a redox proteomic study quantifying cysteine residues susceptible to oxidation. 4784 cysteine-containing peptides could be assigned and quantified in this thesis. Of those, I identified 76 cysteine residues that were differentially oxidized upon T cell activation. Components of mitochondrial metabolism, cytoskeleton dynamics and zinc binding were enriched. As zinc finger proteins are known to mediate transcription/translation and can be modulated via the exclusion of the coordinated zinc ion which is known to dissociate following an increase in oxidative milieu I hypothesized an impact on protein turnover. To investigate mtROS-dependent translation, I employed the non-canonical amino acid (NCAAs), Azidohomoalanine (AHA), which incorporates into newly synthesized proteins and can be used to enrich translated proteins to explore changes in mtROS dependent protein transcription. 376 newly synthesized proteins from all conditions were identified, which allowed me to characterize the impact of mtROS on downstream transcription following activation. I identified two groups of proteins, which were antioxidants and proteins that upregulated NF-κB which is known to upregulate antioxidant processes and T cell activators which are known to be upregulated during T cell activation to modulate transcription factors, such as NFAT binding proteins. This work has highlighted the impact of mtROS on T cell expansion through NFAT and effector function through NF-κB. Taken together, this thesis has characterized the mechanistic impact of mtROS in T cell activation as well as the downstream regulatory processes, which constitute an mtROS dependent signaling network to achieve full T cell activation." @default.
• W3048963208 created "2020-08-18" @default.
• W3048963208 creator A5050930433 @default.
• W3048963208 date "2020-08-12" @default.
• W3048963208 modified "2023-09-26" @default.
• W3048963208 title "Respirasome-mediated Activation of T-Lymphocytes" @default.
• W3048963208 cites W1431954835 @default.
• W3048963208 cites W1495595397 @default.
• W3048963208 cites W1504160775 @default.
• W3048963208 cites W1508685549 @default.
• W3048963208 cites W1542980948 @default.
• W3048963208 cites W1543069842 @default.
• W3048963208 cites W1546966466 @default.
• W3048963208 cites W1556857434 @default.
• W3048963208 cites W1564060069 @default.
• W3048963208 cites W1565329973 @default.
• W3048963208 cites W1576799475 @default.
• W3048963208 cites W1580734844 @default.
• W3048963208 cites W1585296580 @default.
• W3048963208 cites W1598659248 @default.
• W3048963208 cites W1600928734 @default.
• W3048963208 cites W1601981272 @default.
• W3048963208 cites W1610775872 @default.
• W3048963208 cites W1685813921 @default.
• W3048963208 cites W1761054167 @default.
• W3048963208 cites W1767073733 @default.
• W3048963208 cites W1780927013 @default.
• W3048963208 cites W1837391132 @default.
• W3048963208 cites W1851279030 @default.
• W3048963208 cites W1912092092 @default.
• W3048963208 cites W1917974963 @default.
• W3048963208 cites W1918238463 @default.
• W3048963208 cites W1921915021 @default.
• W3048963208 cites W1948683976 @default.
• W3048963208 cites W1961431597 @default.
• W3048963208 cites W1965285497 @default.
• W3048963208 cites W1965879808 @default.
• W3048963208 cites W1966160892 @default.
• W3048963208 cites W1967867591 @default.
• W3048963208 cites W1969665448 @default.
• W3048963208 cites W1970479761 @default.
• W3048963208 cites W1972194912 @default.
• W3048963208 cites W1973563968 @default.
• W3048963208 cites W1977678718 @default.
• W3048963208 cites W1981311979 @default.
• W3048963208 cites W1981593008 @default.
• W3048963208 cites W1981852365 @default.
• W3048963208 cites W1982753455 @default.
• W3048963208 cites W1983016535 @default.
• W3048963208 cites W1984379998 @default.
• W3048963208 cites W1989266322 @default.
• W3048963208 cites W1989899886 @default.
• W3048963208 cites W1990863238 @default.
• W3048963208 cites W1991105829 @default.
• W3048963208 cites W1991933429 @default.
• W3048963208 cites W1992734523 @default.
• W3048963208 cites W1993699540 @default.
• W3048963208 cites W1994246321 @default.
• W3048963208 cites W2000523097 @default.
• W3048963208 cites W2005571932 @default.
• W3048963208 cites W2010643291 @default.
• W3048963208 cites W2014389088 @default.
• W3048963208 cites W2015650234 @default.
• W3048963208 cites W2018367629 @default.
• W3048963208 cites W2024812783 @default.
• W3048963208 cites W2025236053 @default.
• W3048963208 cites W2025343433 @default.
• W3048963208 cites W2026831884 @default.
• W3048963208 cites W2028402847 @default.
• W3048963208 cites W2028447166 @default.
• W3048963208 cites W2030290200 @default.
• W3048963208 cites W2031869158 @default.
• W3048963208 cites W2032419047 @default.
• W3048963208 cites W2037169576 @default.
• W3048963208 cites W2037279230 @default.
• W3048963208 cites W2037525195 @default.
• W3048963208 cites W2037627101 @default.
• W3048963208 cites W2041723553 @default.
• W3048963208 cites W2042662175 @default.
• W3048963208 cites W2043096460 @default.
• W3048963208 cites W2043881813 @default.
• W3048963208 cites W2045484875 @default.
• W3048963208 cites W2046034086 @default.
• W3048963208 cites W2046442704 @default.
• W3048963208 cites W2046722633 @default.
• W3048963208 cites W2048505422 @default.
• W3048963208 cites W2048671791 @default.
• W3048963208 cites W2049973825 @default.
• W3048963208 cites W2050897300 @default.
• W3048963208 cites W2050994990 @default.
• W3048963208 cites W2052154747 @default.
• W3048963208 cites W2053375934 @default.
• W3048963208 cites W2053377061 @default.
• W3048963208 cites W2054633904 @default.
• W3048963208 cites W2056357865 @default.
• W3048963208 cites W2058791890 @default.
• W3048963208 cites W2062063518 @default.
• W3048963208 cites W2064070262 @default.
• W3048963208 cites W2064815984 @default.
• W3048963208 cites W2065306454 @default.

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