Matches in SemOpenAlex for { <https://semopenalex.org/work/W3049006918> ?p ?o ?g. }
- W3049006918 endingPage "106032" @default.
- W3049006918 startingPage "106032" @default.
- W3049006918 abstract "Tolypocladium ophioglossoides is a rare and valuable fungus extensively used in Chinese medicine for relieving postmenopausal syndrome in women yet its bioactive molecules are unknown. To explore its molecular mechanisms, we have developed a reliable Agrobacterium-mediated transformation system using the selective marker: the chlorimuron ethyl-resistance gene sur. For this purpose, we firstly constructed a T-DNA binary vector system and then improved the transformation efficiency by optimizing conditional parameters including the Agrobacterium tumefaciens concentration, the conidia number of T. ophioglossoides, the co-culture time and the concentration of acetosyringone. Furthermore, we have knocked-out the ku70 gene，which is a key gene in non-homologous end joining (NHEJ) DNA repair pathway，and the effect of the length of the homologous arms (HA) on the genetic transformation efficacy was also examined, which increased by 60% when HA was about 3 kb in length. Our results suggest that the genetic transformation system is efficient and feasible for the truffle-parasite fungus T. ophioglossoides, which can further be used in large-scale experiments for characterization of genes of interest in future work." @default.
- W3049006918 created "2020-08-21" @default.
- W3049006918 creator A5024363857 @default.
- W3049006918 creator A5039868182 @default.
- W3049006918 creator A5058439661 @default.
- W3049006918 creator A5064029973 @default.
- W3049006918 creator A5079817035 @default.
- W3049006918 date "2020-09-01" @default.
- W3049006918 modified "2023-10-18" @default.
- W3049006918 title "An efficient genetic transformation system for Chinese medicine fungus Tolypocladium ophioglossoides" @default.
- W3049006918 cites W1495126185 @default.
- W3049006918 cites W1581774010 @default.
- W3049006918 cites W1590757915 @default.
- W3049006918 cites W1592427016 @default.
- W3049006918 cites W1883003611 @default.
- W3049006918 cites W1914977630 @default.
- W3049006918 cites W1927834828 @default.
- W3049006918 cites W1968036272 @default.
- W3049006918 cites W1974618342 @default.
- W3049006918 cites W1975558044 @default.
- W3049006918 cites W1982049047 @default.
- W3049006918 cites W1992716708 @default.
- W3049006918 cites W1994663284 @default.
- W3049006918 cites W2027741551 @default.
- W3049006918 cites W2029979826 @default.
- W3049006918 cites W2030485649 @default.
- W3049006918 cites W2032463086 @default.
- W3049006918 cites W2034173921 @default.
- W3049006918 cites W2040845548 @default.
- W3049006918 cites W2042875709 @default.
- W3049006918 cites W2045439285 @default.
- W3049006918 cites W2049448315 @default.
- W3049006918 cites W2049453842 @default.
- W3049006918 cites W2051415114 @default.
- W3049006918 cites W2055620439 @default.
- W3049006918 cites W2055916106 @default.
- W3049006918 cites W2062363141 @default.
- W3049006918 cites W2075161757 @default.
- W3049006918 cites W2076364014 @default.
- W3049006918 cites W2077196478 @default.
- W3049006918 cites W2101962513 @default.
- W3049006918 cites W2105373127 @default.
- W3049006918 cites W2116008187 @default.
- W3049006918 cites W2118532777 @default.
- W3049006918 cites W2141740840 @default.
- W3049006918 cites W2142172464 @default.
- W3049006918 cites W2177353623 @default.
- W3049006918 cites W2590430027 @default.
- W3049006918 cites W2790704082 @default.
- W3049006918 cites W2793744420 @default.
- W3049006918 cites W2894683328 @default.
- W3049006918 cites W2905340019 @default.
- W3049006918 cites W2911349294 @default.
- W3049006918 cites W30265360 @default.
- W3049006918 cites W70646997 @default.
- W3049006918 cites W2061839242 @default.
- W3049006918 doi "https://doi.org/10.1016/j.mimet.2020.106032" @default.
- W3049006918 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32805368" @default.
- W3049006918 hasPublicationYear "2020" @default.
- W3049006918 type Work @default.
- W3049006918 sameAs 3049006918 @default.
- W3049006918 citedByCount "5" @default.
- W3049006918 countsByYear W30490069182022 @default.
- W3049006918 countsByYear W30490069182023 @default.
- W3049006918 crossrefType "journal-article" @default.
- W3049006918 hasAuthorship W3049006918A5024363857 @default.
- W3049006918 hasAuthorship W3049006918A5039868182 @default.
- W3049006918 hasAuthorship W3049006918A5058439661 @default.
- W3049006918 hasAuthorship W3049006918A5064029973 @default.
- W3049006918 hasAuthorship W3049006918A5079817035 @default.
- W3049006918 hasConcept C102744134 @default.
- W3049006918 hasConcept C104317684 @default.
- W3049006918 hasConcept C204241405 @default.
- W3049006918 hasConcept C2776162087 @default.
- W3049006918 hasConcept C2779656422 @default.
- W3049006918 hasConcept C2780874738 @default.
- W3049006918 hasConcept C54355233 @default.
- W3049006918 hasConcept C552990157 @default.
- W3049006918 hasConcept C70721500 @default.
- W3049006918 hasConcept C86803240 @default.
- W3049006918 hasConcept C89423630 @default.
- W3049006918 hasConceptScore W3049006918C102744134 @default.
- W3049006918 hasConceptScore W3049006918C104317684 @default.
- W3049006918 hasConceptScore W3049006918C204241405 @default.
- W3049006918 hasConceptScore W3049006918C2776162087 @default.
- W3049006918 hasConceptScore W3049006918C2779656422 @default.
- W3049006918 hasConceptScore W3049006918C2780874738 @default.
- W3049006918 hasConceptScore W3049006918C54355233 @default.
- W3049006918 hasConceptScore W3049006918C552990157 @default.
- W3049006918 hasConceptScore W3049006918C70721500 @default.
- W3049006918 hasConceptScore W3049006918C86803240 @default.
- W3049006918 hasConceptScore W3049006918C89423630 @default.
- W3049006918 hasFunder F4320321001 @default.
- W3049006918 hasFunder F4320322927 @default.
- W3049006918 hasFunder F4320338469 @default.
- W3049006918 hasLocation W30490069181 @default.
- W3049006918 hasOpenAccess W3049006918 @default.
- W3049006918 hasPrimaryLocation W30490069181 @default.