FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3049017880> ?p ?o ?g. }

• W3049017880 endingPage "1576" @default.
• W3049017880 startingPage "1570" @default.
• W3049017880 abstract "Because active cells present higher abundance of ribosomal RNA (rRNA) than rDNA (rRNA genes), data obtained with rDNA-based quantitative polymerase chain reaction (qPCR) and rRNA-based qPCR (RT-qPCR) were correlated to search for active bacteria after chemomechanical procedures (CMP). In addition, the ability of both assays to detect bacteria in endodontic samples was evaluated.Samples were taken from 40 teeth with primary endodontic infections before (S1) and after CMP (S2). DNA and cDNA (synthetized from RNA) were used as templates for qPCR using universal primers for bacteria and species-specific primers for Bacteroidaceae sp. HOT-272, Cutibacterium acnes, Selenomonas spp., and Enterococcus faecalis.After CMP, there was a drastic reduction in the number of total bacteria, Selenomonas spp., and E. faecalis, whereas no significant difference was observed for the levels of Bacteroidaceae sp. HOT-272 and C. acnes. The concentration of rRNA copies in S2 samples was significantly higher than the corresponding levels of rDNA for assays targeting total bacteria, Bacteroidaceae sp. HOT-272, and C. acnes (P < .05), indicating persistence of active bacteria. The rDNA-based qPCR presented low sensitivity and high specificity when compared with RT-qPCR. For most assays, samples positive for rDNA were also positive for rRNA (positive predictive value = 100%).CMP was effective in reducing levels but not the metabolic activity of total bacteria. Bacteroidaceae sp. HOT-272 and C. acnes were active members of the persistent community. Although less sensitive than RT-qPCR, most rDNA-based qPCR assays had a low risk of providing false-positive results in postinstrumentation samples." @default.
• W3049017880 created "2020-08-21" @default.
• W3049017880 creator A5003830717 @default.
• W3049017880 creator A5012965651 @default.
• W3049017880 creator A5017113370 @default.
• W3049017880 creator A5021310361 @default.
• W3049017880 creator A5024700275 @default.
• W3049017880 creator A5037429824 @default.
• W3049017880 creator A5037768049 @default.
• W3049017880 creator A5057834674 @default.
• W3049017880 creator A5071108322 @default.
• W3049017880 creator A5073524761 @default.
• W3049017880 creator A5075677245 @default.
• W3049017880 creator A5091419070 @default.
• W3049017880 date "2020-11-01" @default.
• W3049017880 modified "2023-09-27" @default.
• W3049017880 title "Analysis of Active Bacteria Persisting after Chemomechanical Procedures: An RNA- and DNA-based Molecular Study" @default.
• W3049017880 cites W1575221654 @default.
• W3049017880 cites W1916466194 @default.
• W3049017880 cites W1986100214 @default.
• W3049017880 cites W1993678032 @default.
• W3049017880 cites W2001082967 @default.
• W3049017880 cites W2004022112 @default.
• W3049017880 cites W2009307819 @default.
• W3049017880 cites W2021295459 @default.
• W3049017880 cites W2022172620 @default.
• W3049017880 cites W2034929800 @default.
• W3049017880 cites W2038277732 @default.
• W3049017880 cites W2108786601 @default.
• W3049017880 cites W2118459813 @default.
• W3049017880 cites W2119077563 @default.
• W3049017880 cites W2120125996 @default.
• W3049017880 cites W2137591193 @default.
• W3049017880 cites W2142849284 @default.
• W3049017880 cites W2143809897 @default.
• W3049017880 cites W2152796130 @default.
• W3049017880 cites W2157291255 @default.
• W3049017880 cites W2196174506 @default.
• W3049017880 cites W2324913614 @default.
• W3049017880 cites W2410652893 @default.
• W3049017880 cites W2560819093 @default.
• W3049017880 cites W2608021981 @default.
• W3049017880 cites W2965481254 @default.
• W3049017880 cites W3009649174 @default.
• W3049017880 cites W3011991063 @default.
• W3049017880 cites W3033502246 @default.
• W3049017880 cites W4213059165 @default.
• W3049017880 doi "https://doi.org/10.1016/j.joen.2020.08.004" @default.
• W3049017880 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32805336" @default.
• W3049017880 hasPublicationYear "2020" @default.
• W3049017880 type Work @default.
• W3049017880 sameAs 3049017880 @default.
• W3049017880 citedByCount "8" @default.
• W3049017880 countsByYear W30490178802021 @default.
• W3049017880 countsByYear W30490178802022 @default.
• W3049017880 countsByYear W30490178802023 @default.
• W3049017880 crossrefType "journal-article" @default.
• W3049017880 hasAuthorship W3049017880A5003830717 @default.
• W3049017880 hasAuthorship W3049017880A5012965651 @default.
• W3049017880 hasAuthorship W3049017880A5017113370 @default.
• W3049017880 hasAuthorship W3049017880A5021310361 @default.
• W3049017880 hasAuthorship W3049017880A5024700275 @default.
• W3049017880 hasAuthorship W3049017880A5037429824 @default.
• W3049017880 hasAuthorship W3049017880A5037768049 @default.
• W3049017880 hasAuthorship W3049017880A5057834674 @default.
• W3049017880 hasAuthorship W3049017880A5071108322 @default.
• W3049017880 hasAuthorship W3049017880A5073524761 @default.
• W3049017880 hasAuthorship W3049017880A5075677245 @default.
• W3049017880 hasAuthorship W3049017880A5091419070 @default.
• W3049017880 hasConcept C104317684 @default.
• W3049017880 hasConcept C153911025 @default.
• W3049017880 hasConcept C2776357993 @default.
• W3049017880 hasConcept C2779489039 @default.
• W3049017880 hasConcept C2779895986 @default.
• W3049017880 hasConcept C42062724 @default.
• W3049017880 hasConcept C48023723 @default.
• W3049017880 hasConcept C49105822 @default.
• W3049017880 hasConcept C523546767 @default.
• W3049017880 hasConcept C54355233 @default.
• W3049017880 hasConcept C55493867 @default.
• W3049017880 hasConcept C67905577 @default.
• W3049017880 hasConcept C86803240 @default.
• W3049017880 hasConcept C89423630 @default.
• W3049017880 hasConceptScore W3049017880C104317684 @default.
• W3049017880 hasConceptScore W3049017880C153911025 @default.
• W3049017880 hasConceptScore W3049017880C2776357993 @default.
• W3049017880 hasConceptScore W3049017880C2779489039 @default.
• W3049017880 hasConceptScore W3049017880C2779895986 @default.
• W3049017880 hasConceptScore W3049017880C42062724 @default.
• W3049017880 hasConceptScore W3049017880C48023723 @default.
• W3049017880 hasConceptScore W3049017880C49105822 @default.
• W3049017880 hasConceptScore W3049017880C523546767 @default.
• W3049017880 hasConceptScore W3049017880C54355233 @default.
• W3049017880 hasConceptScore W3049017880C55493867 @default.
• W3049017880 hasConceptScore W3049017880C67905577 @default.
• W3049017880 hasConceptScore W3049017880C86803240 @default.
• W3049017880 hasConceptScore W3049017880C89423630 @default.
• W3049017880 hasFunder F4320320997 @default.

• next