FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3049095145> ?p ?o ?g. }

• W3049095145 endingPage "127" @default.
• W3049095145 startingPage "115" @default.
• W3049095145 abstract "Background: The type 2 deiodinase (DIO2) converts thyroxine to 3,3',5-triiodothyronine (T3), modulating intracellular T3. An increase in DIO2 within muscle stem cells during skeletal muscle regeneration leads to T3-dependent potentiation of differentiation. The muscle stem cell niche comprises numerous cell types, which coordinate the regeneration process. For example, muscle stem cells provide secretory signals stimulating endothelial cell-mediated vascular repair, and, in turn, endothelial cells promote muscle stem differentiation. We hypothesized that Dio2 loss in muscle stem cells directly impairs muscle stem cell-endothelial cell communication, leading to downstream disruption of endothelial cell function. Methods: We assessed the production of proangiogenic factors in differentiated C2C12 cells and in a C2C12 cell line without Dio2 (D2KO C2C12) by real-time quantitative-polymerase chain reaction and enzyme-linked immunosorbent assay. Conditioned medium (CM) was collected daily in parallel to evaluate its effects on human umbilical vein endothelial cell (HUVEC) proliferation, migration and chemotaxis, and vascular network formation. The effects of T3-treatment on vascular endothelial growth factor (Vegfa) mRNA expression in C2C12 cells and mouse muscle were assessed. Chromatin immunoprecipitation (ChIP) identified thyroid hormone receptor (TR) binding to the Vegfa gene. Using mice with a targeted disruption of Dio2 (D2KO mice), we determined endothelial cell number by immunohistochemistry/flow cytometry and evaluated related gene expression in both uninjured and injured skeletal muscle. Results: In differentiated D2KO C2C12 cells, Vegfa expression was 46% of wildtype (WT) C2C12 cells, while secreted VEGF was 45%. D2KO C2C12 CM exhibited significantly less proangiogenic effects on HUVECs. In vitro and in vivo T3 treatment of C2C12 cells and WT mice, and ChIP using antibodies against TRα, indicated that Vegfa is a direct genomic T3 target. In uninjured D2KO soleus muscle, Vegfa expression was decreased by 28% compared with WT mice, while endothelial cell numbers were decreased by 48%. Seven days after skeletal muscle injury, D2KO mice had 36% fewer endothelial cells, coinciding with an 83% decrease in Vegfa expression in fluorescence-activated cell sorting purified muscle stem cells. Conclusion:Dio2 loss in the muscle stem cell impairs muscle stem cell-endothelial cell crosstalk via changes in the T3-responsive gene Vegfa, leading to downstream impairment of endothelial cell function both in vitro and in vivo." @default.
• W3049095145 created "2020-08-21" @default.
• W3049095145 creator A5000993558 @default.
• W3049095145 creator A5009426718 @default.
• W3049095145 creator A5040787757 @default.
• W3049095145 creator A5046787578 @default.
• W3049095145 creator A5052716690 @default.
• W3049095145 creator A5054271470 @default.
• W3049095145 creator A5056138634 @default.
• W3049095145 creator A5058240948 @default.
• W3049095145 creator A5063763726 @default.
• W3049095145 creator A5067415891 @default.
• W3049095145 creator A5068979800 @default.
• W3049095145 creator A5090782810 @default.
• W3049095145 date "2021-01-01" @default.
• W3049095145 modified "2023-09-26" @default.
• W3049095145 title "A Type 2 Deiodinase-Dependent Increase in <i>Vegfa</i> Mediates Myoblast-Endothelial Cell Crosstalk During Skeletal Muscle Regeneration" @default.
• W3049095145 cites W1679229092 @default.
• W3049095145 cites W1822333268 @default.
• W3049095145 cites W1942265307 @default.
• W3049095145 cites W1973276229 @default.
• W3049095145 cites W1978818320 @default.
• W3049095145 cites W1981948323 @default.
• W3049095145 cites W1982650718 @default.
• W3049095145 cites W1990115887 @default.
• W3049095145 cites W1991535596 @default.
• W3049095145 cites W1991958278 @default.
• W3049095145 cites W1992032967 @default.
• W3049095145 cites W1999058632 @default.
• W3049095145 cites W2000292756 @default.
• W3049095145 cites W2002158046 @default.
• W3049095145 cites W2005817040 @default.
• W3049095145 cites W2010210770 @default.
• W3049095145 cites W2013880145 @default.
• W3049095145 cites W2015975709 @default.
• W3049095145 cites W2021068750 @default.
• W3049095145 cites W2036204980 @default.
• W3049095145 cites W2038474826 @default.
• W3049095145 cites W2040356368 @default.
• W3049095145 cites W2044851440 @default.
• W3049095145 cites W2054297815 @default.
• W3049095145 cites W2058153407 @default.
• W3049095145 cites W2063059513 @default.
• W3049095145 cites W2069377324 @default.
• W3049095145 cites W2083456009 @default.
• W3049095145 cites W2083941402 @default.
• W3049095145 cites W2084889190 @default.
• W3049095145 cites W2085962065 @default.
• W3049095145 cites W2100744265 @default.
• W3049095145 cites W2101428085 @default.
• W3049095145 cites W2137072568 @default.
• W3049095145 cites W2140166139 @default.
• W3049095145 cites W2142718693 @default.
• W3049095145 cites W2156497704 @default.
• W3049095145 cites W2157364879 @default.
• W3049095145 cites W2168537881 @default.
• W3049095145 cites W2268016365 @default.
• W3049095145 cites W2304555545 @default.
• W3049095145 cites W2335855963 @default.
• W3049095145 cites W2345645274 @default.
• W3049095145 cites W2346538067 @default.
• W3049095145 cites W2347039715 @default.
• W3049095145 cites W2425680121 @default.
• W3049095145 cites W2490643024 @default.
• W3049095145 cites W2593889687 @default.
• W3049095145 cites W2605884099 @default.
• W3049095145 cites W2747611974 @default.
• W3049095145 cites W2753821073 @default.
• W3049095145 cites W2772088064 @default.
• W3049095145 cites W2790493109 @default.
• W3049095145 cites W2883070801 @default.
• W3049095145 cites W2894687190 @default.
• W3049095145 cites W2895137021 @default.
• W3049095145 cites W2921434986 @default.
• W3049095145 cites W2928663114 @default.
• W3049095145 cites W2943182825 @default.
• W3049095145 cites W2946144257 @default.
• W3049095145 cites W2946272758 @default.
• W3049095145 cites W2954213593 @default.
• W3049095145 cites W2973788709 @default.
• W3049095145 cites W2981507763 @default.
• W3049095145 cites W2989535528 @default.
• W3049095145 cites W2990330711 @default.
• W3049095145 cites W2993667222 @default.
• W3049095145 cites W2998584009 @default.
• W3049095145 cites W88652696 @default.
• W3049095145 doi "https://doi.org/10.1089/thy.2020.0291" @default.
• W3049095145 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7840309" @default.
• W3049095145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32787533" @default.
• W3049095145 hasPublicationYear "2021" @default.
• W3049095145 type Work @default.
• W3049095145 sameAs 3049095145 @default.
• W3049095145 citedByCount "9" @default.
• W3049095145 countsByYear W30490951452021 @default.
• W3049095145 countsByYear W30490951452022 @default.
• W3049095145 countsByYear W30490951452023 @default.
• W3049095145 crossrefType "journal-article" @default.
• W3049095145 hasAuthorship W3049095145A5000993558 @default.

• next