Matches in SemOpenAlex for { <https://semopenalex.org/work/W3049149110> ?p ?o ?g. }
- W3049149110 abstract "ABSTRACT Munc13 is a large banana-shaped soluble protein that is involved in the regulation of synaptic vesicle docking and fusion. Recent studies suggested that multiple copies of Munc13 form nanoassemblies in active zones of neurons. However, it is not known if such clustering is an inherent self-assembly property of Munc13 or whether Munc13 clusters indirectly by multivalent binding to synaptic vesicles or specific plasma membrane domains at docking sites in the active zone. The functional significance of putative Munc13 clustering is also unknown. Here we report that nano-clustering is an inherent property of Munc13, and is indeed required for vesicle binding to bilayers containing Munc13. Pure Munc13 reconstituted onto supported lipid bilayers assembled into clusters containing from 2 to ∼20 copies as revealed by a combination of quantitative TIRF microscopy and step-wise photobleaching. Surprisingly, only clusters a minimum of 6 copies of Munc13 were capable of efficiently capturing and retaining small unilamellar vesicles. The C-terminal C 2 C domain of Munc13 is not required for Munc13 clustering, but is required for efficient vesicle capture." @default.
- W3049149110 created "2020-08-21" @default.
- W3049149110 creator A5010709531 @default.
- W3049149110 creator A5024304891 @default.
- W3049149110 creator A5033967319 @default.
- W3049149110 creator A5038337581 @default.
- W3049149110 creator A5045553702 @default.
- W3049149110 creator A5045784589 @default.
- W3049149110 creator A5081844561 @default.
- W3049149110 date "2020-08-17" @default.
- W3049149110 modified "2023-09-30" @default.
- W3049149110 title "Vesicle capture by discrete self-assembled clusters of membrane-bound Munc13" @default.
- W3049149110 cites W1497380575 @default.
- W3049149110 cites W1618714882 @default.
- W3049149110 cites W1822005186 @default.
- W3049149110 cites W1977079697 @default.
- W3049149110 cites W1978302530 @default.
- W3049149110 cites W1990558068 @default.
- W3049149110 cites W1994381304 @default.
- W3049149110 cites W2003782999 @default.
- W3049149110 cites W2018461674 @default.
- W3049149110 cites W2020222865 @default.
- W3049149110 cites W2021285837 @default.
- W3049149110 cites W2032069841 @default.
- W3049149110 cites W2042089363 @default.
- W3049149110 cites W2084471484 @default.
- W3049149110 cites W2086919645 @default.
- W3049149110 cites W2088861631 @default.
- W3049149110 cites W2094479838 @default.
- W3049149110 cites W2140717014 @default.
- W3049149110 cites W2159565935 @default.
- W3049149110 cites W2165489086 @default.
- W3049149110 cites W2233262349 @default.
- W3049149110 cites W2298030758 @default.
- W3049149110 cites W2403577250 @default.
- W3049149110 cites W2412917758 @default.
- W3049149110 cites W2509145907 @default.
- W3049149110 cites W2581093640 @default.
- W3049149110 cites W2581328787 @default.
- W3049149110 cites W2587405972 @default.
- W3049149110 cites W2613882793 @default.
- W3049149110 cites W2738720031 @default.
- W3049149110 cites W2740623655 @default.
- W3049149110 cites W2749952289 @default.
- W3049149110 cites W2755745480 @default.
- W3049149110 cites W2764246409 @default.
- W3049149110 cites W2772190072 @default.
- W3049149110 cites W2774185546 @default.
- W3049149110 cites W2793331272 @default.
- W3049149110 cites W2904293622 @default.
- W3049149110 cites W2910595951 @default.
- W3049149110 cites W2917060214 @default.
- W3049149110 cites W2984047455 @default.
- W3049149110 cites W2996914143 @default.
- W3049149110 doi "https://doi.org/10.1101/2020.08.17.254821" @default.
- W3049149110 hasPublicationYear "2020" @default.
- W3049149110 type Work @default.
- W3049149110 sameAs 3049149110 @default.
- W3049149110 citedByCount "2" @default.
- W3049149110 countsByYear W30491491102020 @default.
- W3049149110 countsByYear W30491491102021 @default.
- W3049149110 crossrefType "posted-content" @default.
- W3049149110 hasAuthorship W3049149110A5010709531 @default.
- W3049149110 hasAuthorship W3049149110A5024304891 @default.
- W3049149110 hasAuthorship W3049149110A5033967319 @default.
- W3049149110 hasAuthorship W3049149110A5038337581 @default.
- W3049149110 hasAuthorship W3049149110A5045553702 @default.
- W3049149110 hasAuthorship W3049149110A5045784589 @default.
- W3049149110 hasAuthorship W3049149110A5081844561 @default.
- W3049149110 hasBestOaLocation W30491491101 @default.
- W3049149110 hasConcept C12554922 @default.
- W3049149110 hasConcept C130316041 @default.
- W3049149110 hasConcept C148785051 @default.
- W3049149110 hasConcept C159110408 @default.
- W3049149110 hasConcept C171250308 @default.
- W3049149110 hasConcept C185592680 @default.
- W3049149110 hasConcept C192562407 @default.
- W3049149110 hasConcept C196330066 @default.
- W3049149110 hasConcept C41625074 @default.
- W3049149110 hasConcept C41685203 @default.
- W3049149110 hasConcept C55493867 @default.
- W3049149110 hasConcept C71924100 @default.
- W3049149110 hasConcept C86803240 @default.
- W3049149110 hasConceptScore W3049149110C12554922 @default.
- W3049149110 hasConceptScore W3049149110C130316041 @default.
- W3049149110 hasConceptScore W3049149110C148785051 @default.
- W3049149110 hasConceptScore W3049149110C159110408 @default.
- W3049149110 hasConceptScore W3049149110C171250308 @default.
- W3049149110 hasConceptScore W3049149110C185592680 @default.
- W3049149110 hasConceptScore W3049149110C192562407 @default.
- W3049149110 hasConceptScore W3049149110C196330066 @default.
- W3049149110 hasConceptScore W3049149110C41625074 @default.
- W3049149110 hasConceptScore W3049149110C41685203 @default.
- W3049149110 hasConceptScore W3049149110C55493867 @default.
- W3049149110 hasConceptScore W3049149110C71924100 @default.
- W3049149110 hasConceptScore W3049149110C86803240 @default.
- W3049149110 hasLocation W30491491101 @default.
- W3049149110 hasOpenAccess W3049149110 @default.
- W3049149110 hasPrimaryLocation W30491491101 @default.
- W3049149110 hasRelatedWork W1997111118 @default.