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- W3049179622 abstract "The development of cost-effective bioreactors and scalable methods for the high quantities of bacteriophage production meets the global demand for phage therapy. This study demonstrates the potential application of a novel PES-STiO2 nanocomposite membrane (NCM) to maintain the bacteriophages, increase the adsorption rate, and productivity in the phage enrichment reactor (PER). A constant volume of freshly grown Escherichia coli cells in tryptic soy broth (TSB) medium (first phase) was tainted with Escherichia phage in PER (second phase) in a cyclic process. Subsequently, the bacteria-phage filtrate and phage lysate samples were removed from the PER through the external cross-flow membrane reactor and the outlet of the PER at each cycle, respectively. The PER performed with the membrane had shown higher phage titers and productivity in the phage lysate at each cycle than those achieved from the PER w/o NCM (control). The phage productivity was increased (∼107 to 1013 PFU mL−1 cycle−1) with the increase in cycles and was higher than those from the control, and other reported reactors. As detected with the electron scanning microscope (SEM) and flux test, the NCM operated with bacteria-phage could able to prevent biofouling without their loss. Thus, the PES-STiO2 nanocomposite membrane integrated PER is simple and can be used at an industrial scale to maintain the volumetric phage production and higher phage productivity." @default.
- W3049179622 created "2020-08-21" @default.
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- W3049179622 date "2020-11-01" @default.
- W3049179622 modified "2023-09-24" @default.
- W3049179622 title "Nanocomposite membrane integrated phage enrichment process for the enhancement of high rate phage infection and productivity" @default.
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- W3049179622 doi "https://doi.org/10.1016/j.bej.2020.107740" @default.
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