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- W3049204511 endingPage "104098" @default.
- W3049204511 startingPage "104098" @default.
- W3049204511 abstract "Aedes aegypti adult females are key vectors of several arboviruses and flight activity plays a central role in mosquito biology and disease transmission. Available methods to quantify mosquito flight usually require special devices and mostly assess spontaneous locomotor activity at individual level. Here, we developed a new method to determine longitudinal untethered adult A. aegypti induced flight activity: the INduced FLight Activity TEst (INFLATE). This method was an adaptation of the “rapid iterative negative geotaxis” assay to assess locomotor activity in Drosophila and explore the spontaneous behavior of mosquitoes to fly following a physical stimulus. Insects were placed on a plastic cage previously divided in four vertical quadrants and flight performance was carried out by tapping cages towards the laboratory bench. After one minute, the number of insects per quadrant was registered by visual inspection and categorized in five different scores. By using INFLATE, we observed that flight performance was not influenced by repeated testing, sex or 5% ethanol intake. However, induced flight activity was strongly affected by aging, blood meal and inhibition of mitochondrial complex I. This simple and rapid method allows the longitudinal assessment of induced flight activity of multiple untethered mosquitoes and may contribute to a better understanding of A. aegypti dispersal biology." @default.
- W3049204511 created "2020-08-21" @default.
- W3049204511 creator A5035971495 @default.
- W3049204511 creator A5062196069 @default.
- W3049204511 date "2020-10-01" @default.
- W3049204511 modified "2023-10-18" @default.
- W3049204511 title "A simple and reliable method for longitudinal assessment of untethered mosquito induced flight activity" @default.
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- W3049204511 doi "https://doi.org/10.1016/j.jinsphys.2020.104098" @default.
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