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• W3049241915 endingPage "10596" @default.
• W3049241915 startingPage "10587" @default.
• W3049241915 abstract "In this work, the copolymer poly[(9,9-dioctylfluorene)-co-(3-hexylthiophene)] was employed as a matrix for immobilizing phytase, aiming at the detection of phytic acid. The copolymer was spread on the air–water interface forming Langmuir monolayers and phytase adsorbed from the aqueous subphase. The interactions between the copolymer and the enzyme components were investigated with surface pressure and surface potential–area isotherms, Brewster angle microscopy, and polarization modulation infrared reflection–absorption spectroscopy (PM-IRRAS). The enzyme could be incorporated in the monolayers from the aqueous subphase, expanding the copolymer films and maintaining its secondary structure. The polymeric films presented a morphological heterogeneous pattern at the air–water interface because of the ability of their chains to fold and entangle, causing inherent defects in the organization as well as unbalanced lateral distribution at the air–water interface because of the formation of aggregates. The interfacial films were transferred to solid supports as Langmuir–Blodgett films and characterized by PM-IRRAS and scanning electronic microscopy, which showed not only the co-transfer of the enzyme but also the maintenance of their heterogeneous morphological pattern. The enzymatic activity of the blended film was analyzed by UV–vis spectroscopy and allowed the estimation of the value of the Michaelis constant (13.08 mM), demonstrating the feasibility of the system to selectively detect phytic acid for biosensing purposes." @default.
• W3049241915 created "2020-08-21" @default.
• W3049241915 creator A5060273187 @default.
• W3049241915 creator A5067502104 @default.
• W3049241915 creator A5073195214 @default.
• W3049241915 date "2020-08-13" @default.
• W3049241915 modified "2023-10-16" @default.
• W3049241915 title "Langmuir and Langmuir–Blodgett Films of Poly[(9,9-dioctylfluorene)-<i>co</i>-(3-hexylthiophene)] for Immobilization of Phytase: Possible Application as a Phytic Acid Sensor" @default.
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• W3049241915 doi "https://doi.org/10.1021/acs.langmuir.0c01941" @default.
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• W3049241915 hasPublicationYear "2020" @default.
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