Matches in SemOpenAlex for { <https://semopenalex.org/work/W3049406219> ?p ?o ?g. }
- W3049406219 abstract "Abstract Corneal endothelial (CE) dysfunction is the main indication for corneal transplantation, an invasive procedure with several limitations. Developing novel strategies to re-activate CE regenerative capacity is, therefore, of fundamental importance. This goal has proved to be challenging as corneal endothelial cells (CEnC) are blocked in the G0/G1 phase of the cell cycle in vivo and, albeit retaining proliferative capacity in vitro, this is further hindered by endothelial-to-mesenchymal transition. Herein we investigated the mechanisms regulating CEnC proliferation in vitro . Comparing the proteome of non-proliferating (in vivo—G0/G1) and proliferating (in vitro—G2/M) rabbit CEnC (rCEnC), 77 proteins, out of 3,328 identified, were differentially expressed in the two groups ( p < 0.005). Literature and Gene Ontology analysis revealed β-catenin and transforming growth factor (TGF-β) pathways to be correlated with the identified proteins. Treatment of rCEnC with a β-catenin activator and inhibitor showed that β-catenin activation was necessary during rCEnC proliferation, but not sufficient for its induction. Furthermore, both pro-proliferative activity of basic fibroblast growth factor and anti-proliferative effects of TGF-β were regulated through β-catenin. Overall, these results provide novel insights into the molecular basis underlying the proliferation process that CEnC re-activate in vitro , consolidating the role of β-catenin and TGF-β ." @default.
- W3049406219 created "2020-08-21" @default.
- W3049406219 creator A5003589003 @default.
- W3049406219 creator A5037627944 @default.
- W3049406219 creator A5039693085 @default.
- W3049406219 creator A5051025613 @default.
- W3049406219 creator A5064289736 @default.
- W3049406219 creator A5081136991 @default.
- W3049406219 creator A5081841860 @default.
- W3049406219 date "2020-08-14" @default.
- W3049406219 modified "2023-10-16" @default.
- W3049406219 title "A fine-tuned β-catenin regulation during proliferation of corneal endothelial cells revealed using proteomics analysis" @default.
- W3049406219 cites W1533864895 @default.
- W3049406219 cites W1737556493 @default.
- W3049406219 cites W1914659192 @default.
- W3049406219 cites W1963482460 @default.
- W3049406219 cites W1970192935 @default.
- W3049406219 cites W1974714162 @default.
- W3049406219 cites W1980303957 @default.
- W3049406219 cites W1982870374 @default.
- W3049406219 cites W1985760249 @default.
- W3049406219 cites W1987131282 @default.
- W3049406219 cites W1990847664 @default.
- W3049406219 cites W1993547614 @default.
- W3049406219 cites W1996571155 @default.
- W3049406219 cites W2002253933 @default.
- W3049406219 cites W2013394636 @default.
- W3049406219 cites W2014536440 @default.
- W3049406219 cites W2014829486 @default.
- W3049406219 cites W2017177065 @default.
- W3049406219 cites W2024215200 @default.
- W3049406219 cites W2025035335 @default.
- W3049406219 cites W2029969565 @default.
- W3049406219 cites W2030095678 @default.
- W3049406219 cites W2030722162 @default.
- W3049406219 cites W2030810926 @default.
- W3049406219 cites W2035252744 @default.
- W3049406219 cites W2037324745 @default.
- W3049406219 cites W2047992261 @default.
- W3049406219 cites W2049265847 @default.
- W3049406219 cites W2063620211 @default.
- W3049406219 cites W2078063257 @default.
- W3049406219 cites W2078325889 @default.
- W3049406219 cites W2079009954 @default.
- W3049406219 cites W2083002009 @default.
- W3049406219 cites W2083446942 @default.
- W3049406219 cites W2083848791 @default.
- W3049406219 cites W2091176739 @default.
- W3049406219 cites W2100465544 @default.
- W3049406219 cites W2102079388 @default.
- W3049406219 cites W2111156691 @default.
- W3049406219 cites W2111566722 @default.
- W3049406219 cites W2114278041 @default.
- W3049406219 cites W2118056125 @default.
- W3049406219 cites W2126423871 @default.
- W3049406219 cites W2127776747 @default.
- W3049406219 cites W2128393954 @default.
- W3049406219 cites W2129558818 @default.
- W3049406219 cites W2144796426 @default.
- W3049406219 cites W2247247147 @default.
- W3049406219 cites W2267317625 @default.
- W3049406219 cites W2460037848 @default.
- W3049406219 cites W2496870112 @default.
- W3049406219 cites W2516974260 @default.
- W3049406219 cites W2520446050 @default.
- W3049406219 cites W2529749728 @default.
- W3049406219 cites W2564526526 @default.
- W3049406219 cites W2569355044 @default.
- W3049406219 cites W2607387172 @default.
- W3049406219 cites W2616193394 @default.
- W3049406219 cites W2617157486 @default.
- W3049406219 cites W2744223148 @default.
- W3049406219 cites W2757699071 @default.
- W3049406219 cites W2766020779 @default.
- W3049406219 cites W2784643852 @default.
- W3049406219 cites W2791975532 @default.
- W3049406219 cites W2793941328 @default.
- W3049406219 cites W2795151620 @default.
- W3049406219 cites W2796370959 @default.
- W3049406219 cites W2802183709 @default.
- W3049406219 cites W2898527450 @default.
- W3049406219 cites W2902699853 @default.
- W3049406219 cites W2915969864 @default.
- W3049406219 cites W2919858631 @default.
- W3049406219 cites W2923631513 @default.
- W3049406219 cites W2946791870 @default.
- W3049406219 cites W373681543 @default.
- W3049406219 cites W4296086914 @default.
- W3049406219 doi "https://doi.org/10.1038/s41598-020-70800-w" @default.
- W3049406219 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7427785" @default.
- W3049406219 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32796906" @default.
- W3049406219 hasPublicationYear "2020" @default.
- W3049406219 type Work @default.
- W3049406219 sameAs 3049406219 @default.
- W3049406219 citedByCount "9" @default.
- W3049406219 countsByYear W30494062192021 @default.
- W3049406219 countsByYear W30494062192022 @default.
- W3049406219 countsByYear W30494062192023 @default.
- W3049406219 crossrefType "journal-article" @default.
- W3049406219 hasAuthorship W3049406219A5003589003 @default.