FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3049408032> ?p ?o ?g. }

• W3049408032 abstract "Abstract Hepatocellular carcinoma (HCC) is the most common liver cancer and is highly malignant. Male prevalence and frequent activation of the Ras signaling pathway are distinct characteristics of HCC. However, the underlying mechanisms remain to be elucidated. By exploring Hras12V transgenic mice showing male-biased hepatocarcinogenesis, we performed a high-throughput comparative proteomic analysis based on tandem-mass-tag (TMT) labeling combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) on the tissue samples obtained from HCC (T) and their paired adjacent precancerous (P) of Hras12V transgenic male and female mice (Ras-Tg) and normal liver (W) of wild-type male and female mice (Non-Tg). The further validation and investigation were performed using quantitative real-time PCR and western blot. Totally, 5193 proteins were quantified, originating from 5733 identified proteins. Finally, 1344 differentially expressed proteins (DEPs) (quantified in all examined samples; |ratios| ≥ 1.5, p < 0.05) were selected for further analysis. Comparison within W, P, and T of males and females indicated that the number of DEPs in males was much higher than that in females. Bioinformatics analyses showed the common and unique cluster-enriched items between sexes, indicating the common and gender-disparate pathways towards HCC. Expression change pattern analysis revealed HCC positive/negative-correlated and ras oncogene positive/negative-correlated DEPs and pathways. In addition, it showed that the ras oncogene gradually and significantly reduced the responses to sex hormones from hepatocytes to hepatoma cells and therefore shrunk the gender disparity between males and females, which may contribute to the cause of the loss of HCC clinical responses to the therapeutic approaches targeting sex hormone pathways. Additionally, gender disparity in the expression levels of key enzymes involved in retinol metabolism and terpenoid backbone/steroid biosynthesis pathways may contribute to male prevalence in hepatocarcinogenesis. Further, the biomarkers, SAA2, Orm2, and Serpina1e, may be sex differences. In conclusion, common and unique DEPs and pathways toward HCC initiated by ras oncogene from sexually dimorphic hepatocytes provide valuable and novel insights into clinical investigation and practice." @default.
• W3049408032 created "2020-08-21" @default.
• W3049408032 creator A5004595455 @default.
• W3049408032 creator A5009318195 @default.
• W3049408032 creator A5014803780 @default.
• W3049408032 creator A5021421905 @default.
• W3049408032 creator A5022073366 @default.
• W3049408032 creator A5023363049 @default.
• W3049408032 creator A5026071964 @default.
• W3049408032 creator A5032016800 @default.
• W3049408032 creator A5043011775 @default.
• W3049408032 creator A5043774051 @default.
• W3049408032 creator A5071363803 @default.
• W3049408032 creator A5073412977 @default.
• W3049408032 creator A5082502067 @default.
• W3049408032 creator A5082857859 @default.
• W3049408032 date "2020-08-13" @default.
• W3049408032 modified "2023-10-12" @default.
• W3049408032 title "Proteomic analysis revealed common, unique and systemic signatures in gender-dependent hepatocarcinogenesis" @default.
• W3049408032 cites W1963982440 @default.
• W3049408032 cites W1971210952 @default.
• W3049408032 cites W1981125361 @default.
• W3049408032 cites W1983771266 @default.
• W3049408032 cites W1992219711 @default.
• W3049408032 cites W1993938603 @default.
• W3049408032 cites W1994884776 @default.
• W3049408032 cites W1996592956 @default.
• W3049408032 cites W2016062855 @default.
• W3049408032 cites W2032743024 @default.
• W3049408032 cites W2033113959 @default.
• W3049408032 cites W2034239471 @default.
• W3049408032 cites W2034887729 @default.
• W3049408032 cites W2037082754 @default.
• W3049408032 cites W2039461477 @default.
• W3049408032 cites W2039845199 @default.
• W3049408032 cites W2042948416 @default.
• W3049408032 cites W2043103546 @default.
• W3049408032 cites W2047353574 @default.
• W3049408032 cites W2054789893 @default.
• W3049408032 cites W2055459745 @default.
• W3049408032 cites W2061683650 @default.
• W3049408032 cites W2067224679 @default.
• W3049408032 cites W2074230554 @default.
• W3049408032 cites W2084928246 @default.
• W3049408032 cites W2091376176 @default.
• W3049408032 cites W2102402727 @default.
• W3049408032 cites W2117502057 @default.
• W3049408032 cites W2118247434 @default.
• W3049408032 cites W2132241298 @default.
• W3049408032 cites W2133225041 @default.
• W3049408032 cites W2133936296 @default.
• W3049408032 cites W2137583488 @default.
• W3049408032 cites W2172083888 @default.
• W3049408032 cites W2304522844 @default.
• W3049408032 cites W2394889347 @default.
• W3049408032 cites W2475299483 @default.
• W3049408032 cites W2541004931 @default.
• W3049408032 cites W2758415074 @default.
• W3049408032 cites W2768855410 @default.
• W3049408032 cites W2769275129 @default.
• W3049408032 cites W2770900079 @default.
• W3049408032 cites W2771940085 @default.
• W3049408032 cites W2778677617 @default.
• W3049408032 cites W2791155615 @default.
• W3049408032 cites W2792809753 @default.
• W3049408032 cites W2804495347 @default.
• W3049408032 cites W2810690816 @default.
• W3049408032 cites W2922937695 @default.
• W3049408032 cites W2946107537 @default.
• W3049408032 cites W2957509699 @default.
• W3049408032 cites W2971316059 @default.
• W3049408032 cites W2982597248 @default.
• W3049408032 cites W2987478061 @default.
• W3049408032 cites W393983619 @default.
• W3049408032 cites W42450160 @default.
• W3049408032 cites W619066236 @default.
• W3049408032 cites W76271618 @default.
• W3049408032 cites W2202632727 @default.
• W3049408032 cites W2789888346 @default.
• W3049408032 doi "https://doi.org/10.1186/s13293-020-00316-5" @default.
• W3049408032 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7427087" @default.
• W3049408032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32792008" @default.
• W3049408032 hasPublicationYear "2020" @default.
• W3049408032 type Work @default.
• W3049408032 sameAs 3049408032 @default.
• W3049408032 citedByCount "9" @default.
• W3049408032 countsByYear W30494080322021 @default.
• W3049408032 countsByYear W30494080322022 @default.
• W3049408032 countsByYear W30494080322023 @default.
• W3049408032 crossrefType "journal-article" @default.
• W3049408032 hasAuthorship W3049408032A5004595455 @default.
• W3049408032 hasAuthorship W3049408032A5009318195 @default.
• W3049408032 hasAuthorship W3049408032A5014803780 @default.
• W3049408032 hasAuthorship W3049408032A5021421905 @default.
• W3049408032 hasAuthorship W3049408032A5022073366 @default.
• W3049408032 hasAuthorship W3049408032A5023363049 @default.
• W3049408032 hasAuthorship W3049408032A5026071964 @default.
• W3049408032 hasAuthorship W3049408032A5032016800 @default.
• W3049408032 hasAuthorship W3049408032A5043011775 @default.
• W3049408032 hasAuthorship W3049408032A5043774051 @default.

• next