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- W3049531957 abstract "Microscale thermometry of aqueous solutions is essential to understand the dynamics of local heat generation and dissipation in chemical and biological systems. A wide variety of fluorescent probes have been developed to map temperature changes with submicrometer resolution, but they often suffer from the uncertainty associated with microenvironment-dependent fluorescent properties. In this work, we develop a label-free ratiometric stimulated Raman scattering (SRS) microscopy technique to quantify microscale temperature by monitoring the O–H Raman stretching modes of water. By tracking the ratio changes of the hydrogen-bonding O–H band and the isosbestic band, we can directly quantify the temperature of water-based environments in real time without exogenous contrast agents. We demonstrate real-time measurement of localized intracellular and extracellular temperature changes due to laser absorption. This high-speed nonlinear optical imaging technique has the potential for in situ microscale imaging of thermogenesis in both chemical and biological systems." @default.
- W3049531957 created "2020-08-21" @default.
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- W3049531957 date "2020-08-13" @default.
- W3049531957 modified "2023-10-16" @default.
- W3049531957 title "Real-Time Microscale Temperature Imaging by Stimulated Raman Scattering" @default.
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- W3049531957 doi "https://doi.org/10.1021/acs.jpclett.0c02029" @default.
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