FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3049669868> ?p ?o ?g. }

• W3049669868 endingPage "15106" @default.
• W3049669868 startingPage "15098" @default.
• W3049669868 abstract "Enzyme assays are important for many applications including clinical diagnostics, functional proteomics, and drug discovery. Current methods for enzymatic activity measurement often suffer from low analytical sensitivity. We developed an ultrasensitive method for the detection of enzymatic activity using Single Molecule Arrays (eSimoa). The eSimoa assay is accomplished by conjugating substrates to paramagnetic beads and measuring the conversion of substrates to products using single molecule analysis. We demonstrated the eSimoa method for the detection of protein kinases, telomerase, histone H3 methyltransferase SET7/9, and polypeptide N-acetylgalactosaminyltransferase with unprecedented sensitivity. In addition, we tested enzyme inhibition and performed theoretical calculations for the binding of inhibitor to its target enzyme and show the need for an ultrasensitive enzymatic assay to evaluate the potency of tight binding inhibitors. The eSimoa assay was successfully used to determine inhibition constants of both bosutinib and dasatinib. Due to the ultrasensitivity of this method, we also were able to measure the kinase activities at the single cell level. We show that the eSimoa assay is a simple, fast, and highly sensitive approach, which can be easily extended to detect a variety of other enzymes, providing a promising platform for enzyme-related fundamental research and inhibitor screening." @default.
• W3049669868 created "2020-08-21" @default.
• W3049669868 creator A5013401641 @default.
• W3049669868 creator A5046220682 @default.
• W3049669868 creator A5056101043 @default.
• W3049669868 date "2020-08-14" @default.
• W3049669868 modified "2023-10-03" @default.
• W3049669868 title "Ultrasensitive Detection of Enzymatic Activity Using Single Molecule Arrays" @default.
• W3049669868 cites W1977455794 @default.
• W3049669868 cites W1997685956 @default.
• W3049669868 cites W2003973314 @default.
• W3049669868 cites W2016853520 @default.
• W3049669868 cites W2019012381 @default.
• W3049669868 cites W2022979136 @default.
• W3049669868 cites W2024265429 @default.
• W3049669868 cites W2026542427 @default.
• W3049669868 cites W2028213695 @default.
• W3049669868 cites W2032737379 @default.
• W3049669868 cites W2041816353 @default.
• W3049669868 cites W2050356003 @default.
• W3049669868 cites W2060383661 @default.
• W3049669868 cites W2065402283 @default.
• W3049669868 cites W2069201630 @default.
• W3049669868 cites W2089193902 @default.
• W3049669868 cites W2092198587 @default.
• W3049669868 cites W2094757982 @default.
• W3049669868 cites W2094859454 @default.
• W3049669868 cites W2095492902 @default.
• W3049669868 cites W2098168395 @default.
• W3049669868 cites W2111510713 @default.
• W3049669868 cites W2121020758 @default.
• W3049669868 cites W2152265294 @default.
• W3049669868 cites W2167573033 @default.
• W3049669868 cites W2168979150 @default.
• W3049669868 cites W2480281834 @default.
• W3049669868 cites W2557332848 @default.
• W3049669868 cites W2681723919 @default.
• W3049669868 cites W2740337666 @default.
• W3049669868 cites W2763194184 @default.
• W3049669868 cites W2793974528 @default.
• W3049669868 cites W2893227468 @default.
• W3049669868 cites W2900850487 @default.
• W3049669868 cites W2903029096 @default.
• W3049669868 cites W2914968800 @default.
• W3049669868 cites W2917868259 @default.
• W3049669868 cites W2976028181 @default.
• W3049669868 cites W573909598 @default.
• W3049669868 doi "https://doi.org/10.1021/jacs.0c06599" @default.
• W3049669868 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7472518" @default.
• W3049669868 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32797755" @default.
• W3049669868 hasPublicationYear "2020" @default.
• W3049669868 type Work @default.
• W3049669868 sameAs 3049669868 @default.
• W3049669868 citedByCount "20" @default.
• W3049669868 countsByYear W30496698682021 @default.
• W3049669868 countsByYear W30496698682022 @default.
• W3049669868 countsByYear W30496698682023 @default.
• W3049669868 crossrefType "journal-article" @default.
• W3049669868 hasAuthorship W3049669868A5013401641 @default.
• W3049669868 hasAuthorship W3049669868A5046220682 @default.
• W3049669868 hasAuthorship W3049669868A5056101043 @default.
• W3049669868 hasBestOaLocation W30496698681 @default.
• W3049669868 hasConcept C161624437 @default.
• W3049669868 hasConcept C181199279 @default.
• W3049669868 hasConcept C184235292 @default.
• W3049669868 hasConcept C185592680 @default.
• W3049669868 hasConcept C21951064 @default.
• W3049669868 hasConcept C2779536868 @default.
• W3049669868 hasConcept C42362537 @default.
• W3049669868 hasConcept C55493867 @default.
• W3049669868 hasConcept C62478195 @default.
• W3049669868 hasConceptScore W3049669868C161624437 @default.
• W3049669868 hasConceptScore W3049669868C181199279 @default.
• W3049669868 hasConceptScore W3049669868C184235292 @default.
• W3049669868 hasConceptScore W3049669868C185592680 @default.
• W3049669868 hasConceptScore W3049669868C21951064 @default.
• W3049669868 hasConceptScore W3049669868C2779536868 @default.
• W3049669868 hasConceptScore W3049669868C42362537 @default.
• W3049669868 hasConceptScore W3049669868C55493867 @default.
• W3049669868 hasConceptScore W3049669868C62478195 @default.
• W3049669868 hasFunder F4320306137 @default.
• W3049669868 hasIssue "35" @default.
• W3049669868 hasLocation W30496698681 @default.
• W3049669868 hasLocation W30496698682 @default.
• W3049669868 hasLocation W30496698683 @default.
• W3049669868 hasOpenAccess W3049669868 @default.
• W3049669868 hasPrimaryLocation W30496698681 @default.
• W3049669868 hasRelatedWork W1850986032 @default.
• W3049669868 hasRelatedWork W1968721630 @default.
• W3049669868 hasRelatedWork W1999859271 @default.
• W3049669868 hasRelatedWork W2000452813 @default.
• W3049669868 hasRelatedWork W2029732200 @default.
• W3049669868 hasRelatedWork W2056322765 @default.
• W3049669868 hasRelatedWork W2080203695 @default.
• W3049669868 hasRelatedWork W2160182407 @default.
• W3049669868 hasRelatedWork W2330215216 @default.
• W3049669868 hasRelatedWork W4242974861 @default.
• W3049669868 hasVolume "142" @default.

• next