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• W3054337399 abstract "A novel luminol electrochemiluminescence (ECL) sensing platform was developed based on the closed indium tin oxide bipolar electrode (ITO BPE) for detecting the biomarkers of squamous cell carcinomas named cytokeratin 19 fragments (CYFRA 21-1). A strategy of in situ-generated coreactant H2O2 and O2 was proposed to enhance luminol ECL intensity. CaO2, possessing a high capacity of self-supplying H2O2 and O2, was encapsulated by ZIF-67, glucose oxidase, and horseradish peroxidase (GOD/HRP-loaded ZIF-67@CaO2). In the presence of glucose, gluconic acid and H2O2 were generated via the catalytic effect of GOD; then, gluconic acid induced the degradation of ZIF-67, leading to the hydrolysis of the unprotected CaO2 to produce both O2 and H2O2. Therefore, the generated coreactants O2 and H2O2, via the oxidation of glucose and the hydrolysis of CaO2, can effectively enhance the luminol ECL behavior. According to the luminol ECL reaction occurring on the anode surface of the ITO BPE, a sandwich immunosensor was fabricated on the anodic ITO BPE and GOD/HRP-loaded ZIF-67@CaO2 as labels incubated with secondary antibodies. Owing to the cathodic reaction rate influencing the anodic reaction rate, Au nanoparticles were electrodeposited on the cathode surface of the ITO BPE, which not only meliorated oxygen reduction but also further enhanced the ECL intensity of luminol. Under optimal experiments, an ECL immunosensor for detecting CYFRA 21-1 with a wider linear range from 0.0075 to 50 ng mL-1 and a detection limit of 1.89 pg mL-1 (S/N = 3) was obtained." @default.
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• W3054337399 date "2020-08-18" @default.
• W3054337399 modified "2023-10-16" @default.
• W3054337399 title "Self-Supply of H₂O₂ and O₂ by Hydrolyzing CaO₂ to Enhance the Electrochemiluminescence of Luminol Based on a Closed Bipolar Electrode" @default.
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• W3054337399 doi "https://doi.org/10.1021/acs.analchem.0c03170" @default.
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