Matches in SemOpenAlex for { <https://semopenalex.org/work/W305499504> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W305499504 endingPage "5920" @default.
- W305499504 startingPage "5916" @default.
- W305499504 abstract "The pH dependence of myo-inositol monophosphatase may indicate a role for histidine residues in the catalytic mechanism (Ganzhorn, A. J., and Chanal, M.-C. (1990) Biochemistry 29, 6065-6071). This possibility was investigated by chemical modification. At pH 6.0 and 25 degrees C, the enzyme was inactivated by diethylpyrocarbonate in a pseudo-first order reaction with a bimolecular rate constant of 0.37 M-1 s-1. Two histidines were modified rapidly with no effect on enzyme activity, while 3 residues were modified at a slower rate corresponding to the rate of inactivation. No noticeable changes in the secondary structure of the enzyme were observed by comparison of circular dichroic spectra before and after modification. Treatment of myo-inositol monophosphatase with diethylpyrocarbonate in the presence of inositol 1-phosphate, Mg2+, and Li+ protected 2 residues from modification and decreased the inactivation rate by about 5-fold. Spectrophotometric analysis, the restoration of enzyme activity by hydroxylamine, and the lack of any inhibitory effect with alkylating agents suggest that inactivation is due solely to modification of histidine. We conclude that a histidine residue is essential for activity and may act as a base catalyst during hydrolysis of the substrate." @default.
- W305499504 created "2016-06-24" @default.
- W305499504 creator A5030740220 @default.
- W305499504 creator A5040122002 @default.
- W305499504 date "1992-03-01" @default.
- W305499504 modified "2023-09-30" @default.
- W305499504 title "The effect of histidine modification on the activity of myo-inositol monophosphatase from bovine brain." @default.
- W305499504 cites W147482384 @default.
- W305499504 cites W1559218659 @default.
- W305499504 cites W1569070386 @default.
- W305499504 cites W1572146622 @default.
- W305499504 cites W1615020669 @default.
- W305499504 cites W1726292056 @default.
- W305499504 cites W1927894522 @default.
- W305499504 cites W1967517885 @default.
- W305499504 cites W1978676513 @default.
- W305499504 cites W1980874857 @default.
- W305499504 cites W1989790643 @default.
- W305499504 cites W1997093700 @default.
- W305499504 cites W2012362734 @default.
- W305499504 cites W2038968490 @default.
- W305499504 cites W2041865332 @default.
- W305499504 cites W2050435420 @default.
- W305499504 cites W2050923935 @default.
- W305499504 cites W2053371581 @default.
- W305499504 cites W2062869162 @default.
- W305499504 cites W2082467761 @default.
- W305499504 cites W2085968319 @default.
- W305499504 cites W2123771690 @default.
- W305499504 cites W2253976052 @default.
- W305499504 cites W2395718832 @default.
- W305499504 cites W4233525121 @default.
- W305499504 cites W70782260 @default.
- W305499504 cites W955725593 @default.
- W305499504 doi "https://doi.org/10.1016/s0021-9258(18)42641-5" @default.
- W305499504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1313422" @default.
- W305499504 hasPublicationYear "1992" @default.
- W305499504 type Work @default.
- W305499504 sameAs 305499504 @default.
- W305499504 citedByCount "15" @default.
- W305499504 countsByYear W3054995042015 @default.
- W305499504 countsByYear W3054995042017 @default.
- W305499504 countsByYear W3054995042023 @default.
- W305499504 crossrefType "journal-article" @default.
- W305499504 hasAuthorship W305499504A5030740220 @default.
- W305499504 hasAuthorship W305499504A5040122002 @default.
- W305499504 hasBestOaLocation W3054995041 @default.
- W305499504 hasConcept C170493617 @default.
- W305499504 hasConcept C181199279 @default.
- W305499504 hasConcept C185592680 @default.
- W305499504 hasConcept C2777427919 @default.
- W305499504 hasConcept C2778460671 @default.
- W305499504 hasConcept C55493867 @default.
- W305499504 hasConceptScore W305499504C170493617 @default.
- W305499504 hasConceptScore W305499504C181199279 @default.
- W305499504 hasConceptScore W305499504C185592680 @default.
- W305499504 hasConceptScore W305499504C2777427919 @default.
- W305499504 hasConceptScore W305499504C2778460671 @default.
- W305499504 hasConceptScore W305499504C55493867 @default.
- W305499504 hasIssue "9" @default.
- W305499504 hasLocation W3054995041 @default.
- W305499504 hasOpenAccess W305499504 @default.
- W305499504 hasPrimaryLocation W3054995041 @default.
- W305499504 hasRelatedWork W1748687783 @default.
- W305499504 hasRelatedWork W1902608271 @default.
- W305499504 hasRelatedWork W1933973922 @default.
- W305499504 hasRelatedWork W1972820280 @default.
- W305499504 hasRelatedWork W2016632542 @default.
- W305499504 hasRelatedWork W2051885497 @default.
- W305499504 hasRelatedWork W2110460577 @default.
- W305499504 hasRelatedWork W2139062079 @default.
- W305499504 hasRelatedWork W2345426289 @default.
- W305499504 hasRelatedWork W2952647966 @default.
- W305499504 hasVolume "267" @default.
- W305499504 isParatext "false" @default.
- W305499504 isRetracted "false" @default.
- W305499504 magId "305499504" @default.
- W305499504 workType "article" @default.