Matches in SemOpenAlex for { <https://semopenalex.org/work/W3055913276> ?p ?o ?g. }
- W3055913276 abstract "Abstract To minimize the cost of sample preparation and genotyping, most genebank genomics studies in self-pollinating species are conducted on a single individual to represent an accession, which may be heterogeneous with larger than expected intra-accession genetic variation. Here, we compared various population genetics parameters among six DNA (leaf) sampling methods on 90 accessions representing a wild species ( O. barthii ), cultivated and landraces ( O. glaberrima , O. sativa ), and improved varieties derived through interspecific hybridizations. A total of 1,527 DNA samples were genotyped with 46,818 polymorphic single nucleotide polymorphisms (SNPs) using DArTseq. Various statistical analyses were performed on eleven datasets corresponding to 5 plants per accession individually and in a bulk (two sets), 10 plants individually and in a bulk (two sets), all 15 plants individually (one set), and a randomly sampled individual repeated six times (six sets). Overall, we arrived at broadly similar conclusions across 11 datasets in terms of SNP polymorphism, heterozygosity/heterogeneity, diversity indices, concordance among genetic dissimilarity matrices, population structure, and genetic differentiation; there were, however, a few discrepancies between some pairs of datasets. Detailed results of each sampling method, the concordance in their outputs, and the technical and cost implications of each method were discussed." @default.
- W3055913276 created "2020-08-24" @default.
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- W3055913276 date "2020-08-19" @default.
- W3055913276 modified "2023-10-16" @default.
- W3055913276 title "Comparisons of sampling methods for assessing intra- and inter-accession genetic diversity in three rice species using genotyping by sequencing" @default.
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- W3055913276 doi "https://doi.org/10.1038/s41598-020-70842-0" @default.
- W3055913276 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7438528" @default.
- W3055913276 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32814806" @default.
- W3055913276 hasPublicationYear "2020" @default.
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