FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3060463208> ?p ?o ?g. }

• W3060463208 endingPage "2129.e4" @default.
• W3060463208 startingPage "2116" @default.
• W3060463208 abstract "Chronic atrophic gastritis can lead to gastric metaplasia and increase risk of gastric adenocarcinoma. Metaplasia is a precancerous lesion associated with an increased risk for carcinogenesis, but the mechanism(s) by which inflammation induces metaplasia are poorly understood. We investigated transcriptional programs in mucous neck cells and chief cells as they progress to metaplasia mice with chronic gastritis.We analyzed previously generated single-cell RNA-sequencing (scRNA-seq) data of gastric corpus epithelium to define transcriptomes of individual epithelial cells from healthy BALB/c mice (controls) and TxA23 mice, which have chronically inflamed stomachs with metaplasia. Chronic gastritis was induced in B6 mice by Helicobacter pylori infection. Gastric tissues from mice and human patients were analyzed by immunofluorescence to verify findings at the protein level. Pseudotime trajectory analysis of scRNA-seq data was used to predict differentiation of normal gastric epithelium to metaplastic epithelium in chronically inflamed stomachs.Analyses of gastric epithelial transcriptomes revealed that gastrokine 3 (Gkn3) mRNA is a specific marker of mouse gastric corpus metaplasia (spasmolytic polypeptide expressing metaplasia, SPEM). Gkn3 mRNA was undetectable in healthy gastric corpus; its expression in chronically inflamed stomachs (from TxA23 mice and mice with Helicobacter pylori infection) identified more metaplastic cells throughout the corpus than previously recognized. Staining of healthy and diseased human gastric tissue samples paralleled these results. Although mucous neck cells and chief cells from healthy stomachs each had distinct transcriptomes, in chronically inflamed stomachs, these cells had distinct transcription patterns that converged upon a pre-metaplastic pattern, which lacked the metaplasia-associated transcripts. Finally, pseudotime trajectory analysis confirmed the convergence of mucous neck cells and chief cells into a pre-metaplastic phenotype that ultimately progressed to metaplasia.In analyses of tissues from chronically inflamed stomachs of mice and humans, we expanded the definition of gastric metaplasia to include Gkn3 mRNA and GKN3-positive cells in the corpus, allowing a more accurate assessment of SPEM. Under conditions of chronic inflammation, chief cells and mucous neck cells are plastic and converge into a pre-metaplastic cell type that progresses to metaplasia." @default.
• W3060463208 created "2020-08-24" @default.
• W3060463208 creator A5002748082 @default.
• W3060463208 creator A5011477031 @default.
• W3060463208 creator A5039992573 @default.
• W3060463208 creator A5040760200 @default.
• W3060463208 creator A5047054370 @default.
• W3060463208 creator A5059467927 @default.
• W3060463208 creator A5081650721 @default.
• W3060463208 creator A5086932115 @default.
• W3060463208 creator A5089707506 @default.
• W3060463208 date "2020-12-01" @default.
• W3060463208 modified "2023-10-16" @default.
• W3060463208 title "Single-Cell Transcriptional Analyses Identify Lineage-Specific Epithelial Responses to Inflammation and Metaplastic Development in the Gastric Corpus" @default.
• W3060463208 cites W1587616579 @default.
• W3060463208 cites W1747811634 @default.
• W3060463208 cites W1757407923 @default.
• W3060463208 cites W1974178621 @default.
• W3060463208 cites W1976671493 @default.
• W3060463208 cites W1981922144 @default.
• W3060463208 cites W1984689597 @default.
• W3060463208 cites W1984883254 @default.
• W3060463208 cites W1985424042 @default.
• W3060463208 cites W1996739849 @default.
• W3060463208 cites W2001559657 @default.
• W3060463208 cites W2010653277 @default.
• W3060463208 cites W2059574805 @default.
• W3060463208 cites W2061928622 @default.
• W3060463208 cites W2066675831 @default.
• W3060463208 cites W2069860553 @default.
• W3060463208 cites W2074358609 @default.
• W3060463208 cites W2091347891 @default.
• W3060463208 cites W2094964346 @default.
• W3060463208 cites W2098837984 @default.
• W3060463208 cites W2108414494 @default.
• W3060463208 cites W2132140024 @default.
• W3060463208 cites W2147705631 @default.
• W3060463208 cites W2149541458 @default.
• W3060463208 cites W2157190168 @default.
• W3060463208 cites W2166368024 @default.
• W3060463208 cites W2170096320 @default.
• W3060463208 cites W2286142055 @default.
• W3060463208 cites W2329545126 @default.
• W3060463208 cites W2592634798 @default.
• W3060463208 cites W2601484101 @default.
• W3060463208 cites W2612386238 @default.
• W3060463208 cites W2747877289 @default.
• W3060463208 cites W2773470735 @default.
• W3060463208 cites W2782400666 @default.
• W3060463208 cites W2788976067 @default.
• W3060463208 cites W2794480084 @default.
• W3060463208 cites W2794521141 @default.
• W3060463208 cites W2895424502 @default.
• W3060463208 cites W2902652978 @default.
• W3060463208 cites W2903374927 @default.
• W3060463208 cites W2919559788 @default.
• W3060463208 cites W2949177718 @default.
• W3060463208 cites W2972278340 @default.
• W3060463208 cites W2999146435 @default.
• W3060463208 cites W4256316091 @default.
• W3060463208 doi "https://doi.org/10.1053/j.gastro.2020.08.027" @default.
• W3060463208 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7725914" @default.
• W3060463208 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32835664" @default.
• W3060463208 hasPublicationYear "2020" @default.
• W3060463208 type Work @default.
• W3060463208 sameAs 3060463208 @default.
• W3060463208 citedByCount "38" @default.
• W3060463208 countsByYear W30604632082021 @default.
• W3060463208 countsByYear W30604632082022 @default.
• W3060463208 countsByYear W30604632082023 @default.
• W3060463208 crossrefType "journal-article" @default.
• W3060463208 hasAuthorship W3060463208A5002748082 @default.
• W3060463208 hasAuthorship W3060463208A5011477031 @default.
• W3060463208 hasAuthorship W3060463208A5039992573 @default.
• W3060463208 hasAuthorship W3060463208A5040760200 @default.
• W3060463208 hasAuthorship W3060463208A5047054370 @default.
• W3060463208 hasAuthorship W3060463208A5059467927 @default.
• W3060463208 hasAuthorship W3060463208A5081650721 @default.
• W3060463208 hasAuthorship W3060463208A5086932115 @default.
• W3060463208 hasAuthorship W3060463208A5089707506 @default.
• W3060463208 hasBestOaLocation W30604632082 @default.
• W3060463208 hasConcept C104317684 @default.
• W3060463208 hasConcept C121608353 @default.
• W3060463208 hasConcept C142724271 @default.
• W3060463208 hasConcept C150194340 @default.
• W3060463208 hasConcept C162317418 @default.
• W3060463208 hasConcept C203014093 @default.
• W3060463208 hasConcept C2776409635 @default.
• W3060463208 hasConcept C2776497702 @default.
• W3060463208 hasConcept C2776914184 @default.
• W3060463208 hasConcept C2778677798 @default.
• W3060463208 hasConcept C2779357193 @default.
• W3060463208 hasConcept C2779422922 @default.
• W3060463208 hasConcept C2780342937 @default.
• W3060463208 hasConcept C2780561475 @default.
• W3060463208 hasConcept C2781443798 @default.
• W3060463208 hasConcept C529295009 @default.
• W3060463208 hasConcept C54355233 @default.

• next