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- W3060588429 abstract "Emulsions offer the means to miniaturize and parallelize high-throughput screening but require a robust method to localize activity-based fluorescent probes in each droplet. Multiplexing probes in droplets is impractical, though highly desirable for identifying library members that possess very specific activity. Here, we present multiplexed probe immobilization on library beads for emulsion screening. During library bead preparation, we quantitated ∼106 primers per bead by fluorescence in situ hybridization, however emulsion PCR yielded only ∼103 gene copies per bead. We leveraged the unextended bead-bound primers to hybridize complementary probe-oligonucleotide heteroconjugates to the library beads. The probe-hybridized bead libraries were then used to program emulsion in vitro transcription/translation reactions and analyzed by FACS to perform multiplexed activity-based screening of trypsin and chymotrypsin mutant libraries for novel proteolytic specificity. The approach’s modularity should permit a high degree of probe multiplexing and appears extensible to other enzyme classes and library types." @default.
- W3060588429 created "2020-08-24" @default.
- W3060588429 creator A5018062150 @default.
- W3060588429 creator A5081641289 @default.
- W3060588429 date "2020-08-17" @default.
- W3060588429 modified "2023-09-27" @default.
- W3060588429 title "Multiplexed Enzyme Activity-Based Probe Display via Hybridization" @default.
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- W3060588429 doi "https://doi.org/10.1021/acscombsci.0c00116" @default.
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