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- W306073491 abstract "This report describes a rapid solid-phase melting curve analysis method based on dynamic allele-specific hybridization (DASH) for single nucleotide polymorphism (SNP) genotyping. Beads with DNA duplexes are immobilized on the surface of a microheater chip. SNP on PCR products were scored, illustrating the sensitivity and robustness of the system. Single-bead detection and multiplexing were performed with a heating rate more than 20 times faster than conventional DASH. Hence, analyses that took more than 15 minutes could be performed in less than 1 minute, enabling ultra-rapid SNP analysis." @default.
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- W306073491 date "2005-01-01" @default.
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- W306073491 title "Ultra-rapid melting curve analysis on beads for high-throughput genotyping of single nucleotide polymorphism" @default.
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