Matches in SemOpenAlex for { <https://semopenalex.org/work/W306876105> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W306876105 endingPage "467" @default.
- W306876105 startingPage "464" @default.
- W306876105 abstract "Accroding to mRNA gene sequence registered in GenBank,a pairs of sepcific primers for the genes of chain α of MHCⅡof duck were designed and synthesized.Using total RNA from duck spleen,the target gene fragment was obtained by RT-PCR,then cloned into the T cloning vector pMD18-T.After being identified by restriction digestion and DNA sequencing,the insert was subcloned into the expression vector pET-28a.Transformed into E.coli BL21,the recombination plasmid were induced to obtain the interest protein.Use electroeluting purified the protein to immunize the mouse for preparing polyclonal antibody.The results showed we cloned the MHCⅡα gene,the obtained 633 bp fragment has 99% identities to the previously identified duck MHCⅡalpa at nucleotide level;successfully constructed the porkaryotic expression vector and the protein expressed efficiently.The protein purified and purity reached 90%.A high titer and specific antibody has been prepared by purified protein,it was proved by the methods of ELISA and Western-blot.All this makes it possible to do further studies of duck MHCⅡgene." @default.
- W306876105 created "2016-06-24" @default.
- W306876105 creator A5012385160 @default.
- W306876105 creator A5057678466 @default.
- W306876105 creator A5057879178 @default.
- W306876105 creator A5068611564 @default.
- W306876105 creator A5073929829 @default.
- W306876105 creator A5086277152 @default.
- W306876105 creator A5090614334 @default.
- W306876105 date "2009-01-01" @default.
- W306876105 modified "2023-09-27" @default.
- W306876105 title "Expression, purification and antibody preparation of duck MHC II α gene." @default.
- W306876105 hasPublicationYear "2009" @default.
- W306876105 type Work @default.
- W306876105 sameAs 306876105 @default.
- W306876105 citedByCount "0" @default.
- W306876105 crossrefType "journal-article" @default.
- W306876105 hasAuthorship W306876105A5012385160 @default.
- W306876105 hasAuthorship W306876105A5057678466 @default.
- W306876105 hasAuthorship W306876105A5057879178 @default.
- W306876105 hasAuthorship W306876105A5068611564 @default.
- W306876105 hasAuthorship W306876105A5073929829 @default.
- W306876105 hasAuthorship W306876105A5086277152 @default.
- W306876105 hasAuthorship W306876105A5090614334 @default.
- W306876105 hasConcept C104317684 @default.
- W306876105 hasConcept C121050878 @default.
- W306876105 hasConcept C153911025 @default.
- W306876105 hasConcept C159654299 @default.
- W306876105 hasConcept C187882448 @default.
- W306876105 hasConcept C19924922 @default.
- W306876105 hasConcept C199360897 @default.
- W306876105 hasConcept C207936829 @default.
- W306876105 hasConcept C33161422 @default.
- W306876105 hasConcept C40767141 @default.
- W306876105 hasConcept C41008148 @default.
- W306876105 hasConcept C54355233 @default.
- W306876105 hasConcept C86803240 @default.
- W306876105 hasConcept C956191 @default.
- W306876105 hasConceptScore W306876105C104317684 @default.
- W306876105 hasConceptScore W306876105C121050878 @default.
- W306876105 hasConceptScore W306876105C153911025 @default.
- W306876105 hasConceptScore W306876105C159654299 @default.
- W306876105 hasConceptScore W306876105C187882448 @default.
- W306876105 hasConceptScore W306876105C19924922 @default.
- W306876105 hasConceptScore W306876105C199360897 @default.
- W306876105 hasConceptScore W306876105C207936829 @default.
- W306876105 hasConceptScore W306876105C33161422 @default.
- W306876105 hasConceptScore W306876105C40767141 @default.
- W306876105 hasConceptScore W306876105C41008148 @default.
- W306876105 hasConceptScore W306876105C54355233 @default.
- W306876105 hasConceptScore W306876105C86803240 @default.
- W306876105 hasConceptScore W306876105C956191 @default.
- W306876105 hasIssue "4" @default.
- W306876105 hasLocation W3068761051 @default.
- W306876105 hasOpenAccess W306876105 @default.
- W306876105 hasPrimaryLocation W3068761051 @default.
- W306876105 hasRelatedWork W2023802249 @default.
- W306876105 hasRelatedWork W2197960734 @default.
- W306876105 hasRelatedWork W2348991363 @default.
- W306876105 hasRelatedWork W2351853462 @default.
- W306876105 hasRelatedWork W2352186925 @default.
- W306876105 hasRelatedWork W2354466098 @default.
- W306876105 hasRelatedWork W2380927272 @default.
- W306876105 hasRelatedWork W2381794601 @default.
- W306876105 hasRelatedWork W2387358509 @default.
- W306876105 hasRelatedWork W2390285715 @default.
- W306876105 hasRelatedWork W2399188697 @default.
- W306876105 hasRelatedWork W2402744584 @default.
- W306876105 hasRelatedWork W2417088459 @default.
- W306876105 hasRelatedWork W2553760270 @default.
- W306876105 hasRelatedWork W2994483582 @default.
- W306876105 hasRelatedWork W3030027439 @default.
- W306876105 hasRelatedWork W3125711110 @default.
- W306876105 hasRelatedWork W3148694062 @default.
- W306876105 hasRelatedWork W3196488882 @default.
- W306876105 hasRelatedWork W3095799487 @default.
- W306876105 hasVolume "29" @default.
- W306876105 isParatext "false" @default.
- W306876105 isRetracted "false" @default.
- W306876105 magId "306876105" @default.
- W306876105 workType "article" @default.