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- W3071137583 abstract "Spidroin has the potential of wide applications in the biomedicine field as a natural biomaterial. Various synthetic fibers with outstanding mechanical properties have been produced from different spidroins. However, studies on the structural analysis or biomimetic exploration of aggregate spidroin (AgSp) remain scarce. Here, three recombinant AgSp1 spidroins (1RP, 1RC, 3RP) were constructed and expressed in Escherichia coli, followed by purification via coupling heating and ammonium sulfate precipitation. Circular dichroism (CD) spectrum-based secondary structural analysis shows that 1RP and 3RP have similar structures (mainly random coil) in water and PB buffer, while 1RC is mainly composed of α-helix structure and HFIP can change all of the recombinant AgSp1 into helix structure. Through the wet-spinning method, six types of synthetic fibers were produced from these three recombinant AgSp1 spidroins. Subsequently, the properties and structures of synthetic fibers were characterized by mechanical testing and ATR-FTIR. Synthetic fibers spun from 3RP have considerable tensile strength and extensibility (∼37.56 MPa and ∼4.5%, respectively). To the best of our knowledge, this is the first synthetic fiber obtained from AgSp spidroin. Our results demonstrated that AgSp1 can be regarded as an available source of spidroin for silklike fiber production and may provide valuable perspectives on the AgSp1 biomimetic process for certain applications." @default.
- W3071137583 created "2020-08-24" @default.
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- W3071137583 date "2020-08-18" @default.
- W3071137583 modified "2023-09-27" @default.
- W3071137583 title "Wet-Spinning Synthetic Fibers from Aggregate Glue: Aggregate Spidroin 1 (AgSp1)" @default.
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- W3071137583 doi "https://doi.org/10.1021/acsabm.0c00619" @default.
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